{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:04:03Z","timestamp":1781010243175,"version":"3.54.1"},"reference-count":72,"publisher":"IWA Publishing","issue":"9","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006756","name":"Brigham Young University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006756","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iwaponline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Infographic summarizing a study on Caldicellulosiruptor bescii pretreatment of manure. It shows background on resistant substrates, an experimental setup with EBP followed by anaerobic digestion (AD) and sampling over 200 days, and results indicating increased hydrogenotrophic methanogens and greater bacterial persistence in AD after pretreatment.<\/jats:p>\n                  <jats:p>Extremophilic biological pretreatment (EBP) of dairy manure using Caldicellulosiruptor bescii at 75\u00b0C dramatically alters the microbial community structure and activity in anaerobic digesters (ADs). This study evaluated microbial abundance and activity in manure, EBP, and ADs at industrial scales using 16S sequencing of rDNA and rRNA. Results revealed that bacterial communities remained largely active and resilient throughout the system, but acetoclastic methanogens were sensitive to EBP treatment and failed to reestablish in ADs. In contrast, hydrogenotrophic methanogens survived EBP and persisted in ADs, although these species are poorly suited to metabolize the acetate-rich products generated by C. bescii in pretreatment. We conclude that the acetate utilization of ADs preceded by EBP is likely to improve if supplemented with acetoclastic methanogens, such as those present in raw manure.<\/jats:p>","DOI":"10.2166\/wst.2026.272","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:25:01Z","timestamp":1778599501000},"page":"1214-1227","update-policy":"https:\/\/doi.org\/10.2166\/iwapcrossmarkpolicypage","source":"Crossref","is-referenced-by-count":0,"title":["Selective survival of anaerobic digester microbes after\n                    <i>C. bescii<\/i>\n                    pretreatment of manure"],"prefix":"10.2166","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3972-7261","authenticated-orcid":false,"given":"Maliea A.","family":"Nipko","sequence":"first","affiliation":[{"name":"a Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1320-4876","authenticated-orcid":false,"given":"Caleb M.","family":"Jensen","sequence":"additional","affiliation":[{"name":"b Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8266-5810","authenticated-orcid":false,"given":"Jakob L.","family":"Young","sequence":"additional","affiliation":[{"name":"c Department of Biology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3908-9318","authenticated-orcid":false,"given":"Alexander D.","family":"Belanger","sequence":"additional","affiliation":[{"name":"a Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0600-8893","authenticated-orcid":false,"given":"Zachary T.","family":"Aanderud","sequence":"additional","affiliation":[{"name":"b Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"945","published-online":{"date-parts":[[2026,5,5]]},"reference":[{"key":"2026060908115740400_WST-D-25-00316C1","first-page":"853","article-title":"Bacterial dormancy is more prevalent in freshwater than hypersaline lakes","volume":"7","year":"2016","journal-title":"Frontiers in Microbiology"},{"key":"2026060908115740400_WST-D-25-00316C2","doi-asserted-by":"crossref","first-page":"117494","DOI":"10.1016\/j.fuel.2020.117494","article-title":"A critical review of pretreatment technologies to enhance anaerobic digestion and energy 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