{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:37:53Z","timestamp":1793144273719,"version":"4.5.0"},"reference-count":28,"publisher":"Japanese Society of Internal Medicine","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intern. Med."],"published-print":{"date-parts":[[2022,2,1]]},"DOI":"10.2169\/internalmedicine.7824-21","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T22:08:49Z","timestamp":1643666929000},"page":"313-322","source":"Crossref","is-referenced-by-count":18,"title":["A Comparison of Line Blots, Enzyme-linked Immunosorbent, and RNA-immunoprecipitation Assays of Antisynthetase Antibodies in Serum Samples from 44 Patients"],"prefix":"10.2169","volume":"61","author":[{"given":"Koichiro","family":"Shinoda","sequence":"first","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiko","family":"Okumura","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Matsui","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reina","family":"Tsuda","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Hounoki","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeaki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Neurology, Keio University School of Medicine, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyuki","family":"Tobe","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, University of Toyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"962","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Love LA, Leff RL, Fraser DD, et al. A new approach to the classification of idiopathic inflammatory myopathy: myositis-specific autoantibodies define useful homogeneous patient groups. Medicine (Baltimore) 70: 360-374, 1991.","DOI":"10.1097\/00005792-199111000-00002"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Koenig M, Fritzler MJ, Targoff IN, Troyanov Y, Senecal JL. Heterogeneity of autoantibodies in 100 patients with autoimmune myositis: insights into clinical features and outcomes. Arthritis Res Ther 9: R78, 2007.","DOI":"10.1186\/ar2276"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Mahler M, Fritzler MJ. Epitope specificity and significance in systemic autoimmune diseases. Ann N Y Acad Sci 1183: 267-287, 2010.","DOI":"10.1111\/j.1749-6632.2009.05127.x"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Mahler M, Miller FW, Fritzler MJ. Idiopathic inflammatory myopathies and the anti-synthetase syndrome: a comprehensive review. Autoimmun Rev 13: 367-371, 2014.","DOI":"10.1016\/j.autrev.2014.01.022"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Yamasaki Y, Yamada H, Nozaki T, et al. Unusually high frequency of autoantibodies to PL-7 associated with milder muscle disease in Japanese patients with polymyositis\/dermatomyositis. Arthritis Rheum 54: 2004-2009, 2006.","DOI":"10.1002\/art.21883"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Hamaguchi Y, Fujimoto M, Matsushita T, et al. Common and distinct clinical features in adult patients with anti-aminoacyl-tRNA synthetase antibodies: heterogeneity within the syndrome. PLOS One 8: e60442, 2013.","DOI":"10.1371\/journal.pone.0060442"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Betteridge Z, Tansley S, Shaddick G, et al. Frequency, mutual exclusivity and clinical associations of myositis autoantibodies in a combined European cohort of idiopathic inflammatory myopathy patients. J autoimmun 101: 48-55, 2019.","DOI":"10.1016\/j.jaut.2019.04.001"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Aggarwal R, Cassidy E, Fertig N, et al. Patients with non-Jo-1 anti-tRNA-synthetase autoantibodies have worse survival than Jo-1 positive patients. Ann Rheum Dis 73: 227-232, 2014.","DOI":"10.1136\/annrheumdis-2012-201800"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Hervier B, Devilliers H, Stanciu R, et al. Hierarchical cluster and survival analyses of antisynthetase syndrome: phenotype and outcome are correlated with anti-tRNA synthetase antibody specificity. Autoimmun Rev 12: 210-217, 2012.","DOI":"10.1016\/j.autrev.2012.06.006"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Zhan X, Yan W, Wang Y, et al. Clinical features of anti-synthetase syndrome associated interstitial lung disease: a retrospective cohort in China. BMC Pulm Med 21: 57, 2021.","DOI":"10.1186\/s12890-021-01399-5"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Cruellas MG, Viana Vdos S, Levy-Neto M, Souza FH, Shinjo SK. Myositis-specific and myositis-associated autoantibody profiles and their clinical associations in a large series of patients with polymyositis and dermatomyositis. Clinics (S\u00e3o Paulo, Brazil) 68: 909-914, 2013.","DOI":"10.6061\/clinics\/2013(07)04"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Hamaguchi Y, Kuwana M, Takehara K. Performance evaluation of a commercial line blot assay system for detection of myositis- and systemic sclerosis-related autoantibodies. Clin Rheumatol 39: 3489-3497, 2020.","DOI":"10.1007\/s10067-020-04973-0"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Mecoli CA, Albayda J, Tiniakou E, et al. Myositis autoantibodies: a comparison of results from the Oklahoma Medical Research Foundation myositis panel to the EUROIMMUN research line blot. Arthritis Rheumatol 72: 192-194, 2020.","DOI":"10.1002\/art.41088"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Nakashima R, Imura Y, Hosono Y, et al. The multicenter study of a new assay for simultaneous detection of multiple anti-aminoacyl-tRNA synthetases in myositis and interstitial pneumonia. PLOS One 9: e85062, 2014.","DOI":"10.1371\/journal.pone.0085062"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Kanda Y. Investigation of the freely available easy-to-use software 'EZR' for medical statistics. Bone Marrow Transplan 48: 452-458, 2013.","DOI":"10.1038\/bmt.2012.244"},{"key":"16","doi-asserted-by":"publisher","unstructured":"16. Yoshifuji H, Fujii T, Kobayashi S, et al. Anti-aminoacyl-tRNA synthetase antibodies in clinical course prediction of interstitial lung disease complicated with idiopathic inflammatory myopathies. Autoimmunity 39: 233-241, 2006.","DOI":"10.1080\/08916930600622884"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Hamaguchi Y, Kuwana M, Takehara K. Comparison of anti-OJ antibody detection assays between an immunoprecipitation assay and line blot assay. Mod Rheumatol 27: 551-552, 2017.","DOI":"10.1080\/14397595.2016.1213947"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Vulsteke JB, Satoh M, Malyavantham K, Bossuyt X, De Langhe E, Mahler M. Anti-OJ autoantibodies: rare or underdetected? Autoimmun Rev 18: 658-664, 2019.","DOI":"10.1016\/j.autrev.2019.05.002"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Noguchi E, Uruha A, Suzuki S, et al. Skeletal muscle involvement in antisynthetase syndrome. JAMA Neurol 74: 992-999, 2017.","DOI":"10.1001\/jamaneurol.2017.0934"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Cavazzana I, Fredi M, Ceribelli A, et al. Testing for myositis specific autoantibodies: comparison between line blot and immunoprecipitation assays in 57 myositis sera. J Immunol Methods 433: 1-5, 2016.","DOI":"10.1016\/j.jim.2016.02.017"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Espinosa-Ortega F, Holmqvist M, Alexanderson H, et al. Comparison of autoantibody specificities tested by a line blot assay and immunoprecipitation-based algorithm in patients with idiopathic inflammatory myopathies. Ann Rheum Dis 78: 858-860, 2019.","DOI":"10.1136\/annrheumdis-2018-214690"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Naniwa T, Tamechika S, Okazaki Y, Maeda S, Kuwana M. Coexistence of anti-melanoma differentiation-associated gene 5 and anti-aminoacyl-transfer RNA synthetase antibodies in a patient with dermatomyositis and rapidly progressive and relapsing interstitial lung disease. Mod Rheumatol Case Rep 1: 3-8, 2017.","DOI":"10.1080\/24725625.2016.1253650"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. To F, Vent\u00edn-Rodr\u00edguez C, Elkhalifa S, Lilleker JB, Chinoy H. Line blot immunoassays in idiopathic inflammatory myopathies: retrospective review of diagnostic accuracy and factors predicting true positive results. BMC Rheumatol 4: 28, 2020.","DOI":"10.1186\/s41927-020-00132-9"},{"key":"24","unstructured":"24. Sasai T, Nakashima R, Ishikawa Y, Isayama T, Mimori T. Anti-aminoacyl-tRNA-synthetase antibodies which are positive by ELISA but negative by RNA-immunoprecipitation suggest different antigen recognition and clinical relevance different from typical anti-synthetase syndrome. Arthritis Rheumatol 70 (suppl 10): 2018."},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Kato T, Miyakawa H, Ishibashi M. Frequency and significance of anti-glutathione S-transferase autoantibody (anti-GST A1-1) in autoimmune hepatitis. J Autoimmun 22: 211-216, 2004.","DOI":"10.1016\/j.jaut.2004.01.003"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Akgul SU, O\u011fuz FS, \u00c7al\u0131\u015fkan Y, et al. The effect of anti-human leukocyte antigen, anti-major histocompatibility complex class 1 chain-related antigen a, and anti-glutathione transferase-T1 antibodies on the long-term survival of renal allograft. Transplan Proc 45: 890-894, 2013.","DOI":"10.1016\/j.transproceed.2013.02.097"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Cartwright GA, Rothel JS, Lightowlers MW. Conventional immunoassays underestimate anti-GST antibody titre. J Immunol Methods 179: 31-35, 1995.","DOI":"10.1016\/0022-1759(94)00267-Z"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Ge Y, Li S, Li S, He L, Lu X, Wang G. Interstitial lung disease is a major characteristic of anti-KS associated ant-synthetase syndrome. Ther Adv Chronic Dis 11: 2040622320968417, 2020.","DOI":"10.1177\/2040622320968417"}],"container-title":["Internal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/internalmedicine\/61\/3\/61_7824-21\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T03:16:06Z","timestamp":1644030966000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/internalmedicine\/61\/3\/61_7824-21\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,1]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.2169\/internalmedicine.7824-21","relation":{},"ISSN":["0918-2918","1349-7235"],"issn-type":[{"value":"0918-2918","type":"print"},{"value":"1349-7235","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,1]]},"article-number":"7824-21"}}