{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T21:33:48Z","timestamp":1772660028883,"version":"3.50.1"},"reference-count":61,"publisher":"Bentham Science Publishers Ltd.","issue":"9","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPB"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Several medicinal plants are identified as therapeutic agents for the\nworld\u2019s most deadly disease cancer. A member of the \"Cucurbitaceae\" family of medicinal\nplants, Citrullus colocynthis (C. colocynthis) has various pharmacological actions.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Aims and Objectives:<\/jats:title>\n<jats:p>In the present study we have focused on the phytochemical analysis, antimicrobial,\nanticancer and in silico investigation of fruit extracts of C. colocynthis. The chloroform,\npure ethanolic and aq. ethanolic extracts of C. colocynthis whole fruit, peel and pulp separately\nhave been investigated.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>The phytochemical analysis revealed the presence of alkaloids, flavonoids, steroids,\nphenols, saponins and glycosides in various parts of the fruit. Some compounds have been identified\nusing GC-MS analysis by comparing with NIST library data. The antimicrobial activity of\nall extracts was checked by agar well diffusion method against five different bacterial strains\nsuch as A. baumannii, K. pneumonia, S. aureus, P. aeruginosa and E. coli. The zone of inhibition\n(ZOI) ranged between 11 mm to 27 mm against different strains.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>The polar solvent extracts (ethanolic and aq. ethanolic extract) of peel showed good\nsensitivity against all bacterial strains as compared to non-polar solvent (chloroform extract),\nwhich showed activity only against Staphylococcus aureus and Pseudomonas aeruginosa. The\ncytotoxic activity of C. colocynthis all extracts against human brain cancer cell lines (U-87) was\nassessed using MTT assay.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>The % cell viability of ethanolic (ET-PL), and aq. ethanolic extract of whole fruit\nand pulp showed promising results. The cancerous cell line U-87 seems to be more sensitive\ntowards polar solvents (ethanolic and aq. ethanolic) pulp extracts than peel. Further, based on invitro\nresults, compounds identified in ET-PP were screened for their potential as antibacterial\nand anticancer agents through molecular docking and MMGBSA studies. These studies strongly\nsupported the in-vitro study results and identified new drug candidates.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/0113892010332012241027022502","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T09:17:13Z","timestamp":1730711833000},"page":"1392-1416","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biochemical Screening, In-vitro and In-silico Characterization of Citrullus colocynthis Fruit Extracts: A Combined Experimental and Computation Study"],"prefix":"10.2174","volume":"26","author":[{"given":"Syeda Aaliya","family":"Shehzadi","sequence":"first","affiliation":[{"name":"Sulaiman Bin Abdullah Aba Al-Khail-Centre for Interdisciplinary Research in Basic Sciences (SA-CIRBS), International\nIslamic University, 44000 Islamabad, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Ayaz","family":"Ashraf","sequence":"additional","affiliation":[{"name":"Sulaiman Bin Abdullah Aba Al-Khail-Centre for Interdisciplinary Research in Basic Sciences (SA-CIRBS), International\nIslamic University, 44000 Islamabad, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3270-4227","authenticated-orcid":true,"given":"Nusrat","family":"Shafiq","sequence":"additional","affiliation":[{"name":"Synthetic and Natural Product Drug Discovery Laboratory,\nDepartment of Chemistry, Government College Women University, Faisalababd, 38000, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Rida","sequence":"additional","affiliation":[{"name":"Synthetic and Natural Product Drug Discovery Laboratory,\nDepartment of Chemistry, Government College Women University, Faisalababd, 38000, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneela","family":"Javed","sequence":"additional","affiliation":[{"name":"Atta-ur-Rahman\nSchool of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Islamabad,\nPakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhan","family":"Younas","sequence":"additional","affiliation":[{"name":"Sulaiman Bin Abdullah Aba Al-Khail-Centre for Interdisciplinary Research in Basic Sciences (SA-CIRBS), International\nIslamic University, 44000 Islamabad, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Waqar-Un-Nisa","sequence":"additional","affiliation":[{"name":"Sulaiman Bin Abdullah Aba Al-Khail-Centre for Interdisciplinary Research in Basic Sciences (SA-CIRBS), International\nIslamic University, 44000 Islamabad, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waleed","family":"Younus","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Hamdard University, Islamabad, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.2174\/138920008785821657","volume":"9","author":"Desai A.","year":"2008","unstructured":"Desai A.; Qazi G.; Ganju R.; El-Tamer M.; Singh J.; Saxena A.; Bedi Y.; Taneja S.; Bhat H.; Medicinal plants and cancer chemoprevention. 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