{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:07:53Z","timestamp":1789373273020,"version":"build-2803163510"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CMM"],"published-print":{"date-parts":[[2026,9,8]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction\/Objective:<\/jats:title>\n                    <jats:p>The repurposing of aprepitant (APR), a clinically\napproved NK-1R antagonist, represents a promising anticancer strategy; however, its\nrapid systemic clearance limits its therapeutic efficacy. This study aimed to design and\nsystematically evaluate PLGA- and ERS-based APR nanoparticles, characterizing\ntheir physicochemical properties, assessing their anticancer potency, and investigating\ntheir specific impact on macrophage-mediated tumor invasion using an in vitro lung\ncancer model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>APR-loaded poly(lactic-co-glycolic acid) (PLGA-APR) and Eudragit\u00ae RS\n100 (ERS-APR) nanoparticles were prepared and characterized for particle size,\npolydispersity index, zeta potential, and encapsulation efficiency. In vitro drug release\nprofiles were evaluated over 24 h. Antiproliferative activity was assessed in A549 lung\ncancer cells and THP-1 macrophages. A co-culture model with M2c-polarized THP-1\nmacrophages was used to investigate the effects on cancer cell invasion. Apoptosisrelated\ngene expression was analyzed to explore the underlying mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Nanoparticles exhibited sizes ranging from 186\u2013244 nm, low polydispersity\nindices, and high encapsulation efficiencies, with distinct surface charges. Sustained\ndrug release over 24 h was observed, whereas free APR dissolved rapidly. Nanoencapsulation\nenhanced antiproliferative activity, with PLGA-APR showing a lower\nIC\u2085\u2080 at 48 h compared with free APR, and demonstrated tumor-selective cytotoxicity.\nAdditionally, the nanoparticle formulations reduced M2c macrophage-induced invasion\nand increased the expression of apoptosis-related genes, including TNF-\u03b1, CASP8,\nand CASP3.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion:<\/jats:title>\n                    <jats:p>These findings suggest that nanoparticle-based delivery enhances the\ntherapeutic potential of APR by improving its antitumor activity and limiting\nmacrophage-mediated pro-tumor interactions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>PLGA- and ERS-based APR nanoparticles demonstrated enhanced\nanticancer activity and represent a promising nanoparticle-based drug repurposing\nstrategy for lung cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2174\/0115665240511001260831114038","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:03:43Z","timestamp":1789124623000},"source":"Crossref","is-referenced-by-count":0,"title":["Nano-Encapsulated Aprepitant Enhances Anticancer Potency in Lung Cancer Cells"],"prefix":"10.2174","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2122-5324","authenticated-orcid":true,"given":"Elif","family":"Kaya-Tilki","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, Anadolu University, Eski\u015fehir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9596-0538","authenticated-orcid":true,"given":"A. Alper","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, Anadolu University, Eski\u015fehir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1534-8117","authenticated-orcid":true,"given":"Selin","family":"Eng\u00fcr-\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services, Tavas Vocational School of Health Services, Pamukkale University, Denizli, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","container-title":["Current Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/0115665240511001260831114038","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/258135\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/0115665240511001260831114038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T07:39:57Z","timestamp":1789371597000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/258135\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":0,"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/0115665240511001260831114038","relation":{},"ISSN":["1566-5240"],"issn-type":[{"value":"1566-5240","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9,8]]}}}