{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:55:28Z","timestamp":1791593728340,"version":"4.3.4"},"reference-count":0,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971817"],"award-info":[{"award-number":["81971817"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jinshan Health Project in Zhenjiang City, China","award":["2021-JSYZ-4"],"award-info":[{"award-number":["2021-JSYZ-4"]}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMP"],"published-print":{"date-parts":[[2025,1,31]]},"abstract":"<jats:sec>\n<jats:title>Aims:<\/jats:title>\n<jats:p>Cardiac fibrosis causes most pathological alterations of cardiomyopathy in diabetes and heart failure patients. The activation and transformation of\ncardiac fibroblasts (CFs) are the main pathological mechanisms of cardiac fibrosis. It has been established that Sirtuin1 (Sirt1) plays a protective\nrole in the pathogenesis of cardiovascular disorders. This study aimed to ascertain the Sirt1 effect on the phenotypic transformation of CFs in\ndiabetes and its possible mechanisms.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Type 1 diabetes was induced in 6-week-old male mice by subcutaneously injecting 50 mg\/kg streptozotocin (STZ, i.p.). Western blotting, collagen\nstaining, and echocardiography were performed to detect protein expression and assess cardiac fibrosis and function in vivo. We used high glucose\n(HG) to culture CFs prior to protein expression measurement in vitro.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Upregulation of Sirt1 expression effectively alleviated the degree of cardiac fibrosis by improving cardiac function in diabetic mice. In vitro\nexperiments revealed that HG decreased the protein expression levels of Sirt1, but increased those of type I collagen and alpha-smooth muscle\nactin (\u03b1-SMA), as well as the transdifferentiation of fibroblasts into myofibroblasts. Further studies confirmed that downregulation of Sirt1\nexpression in the HG environment reduced the protein kinase-B (Akt) phosphorylation, thereby promoting the transdifferentiation of CFs into\nmyofibroblasts coupled with the deterioration of cardiac function.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Diabetes mellitus leads to downregulation of Sirt1 protein expression in CFs and decreased Akt phosphorylation, which promotes the\ntransdifferentiation of CFs into myofibroblasts, the pathological process of cardiac fibrosis, and mediates the incidence and development of\ndiabetic cardiomyopathy.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/0118761429353519250106115016","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T07:46:57Z","timestamp":1736840817000},"update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Sirt1 Regulates Phenotypic Transformation of Diabetic Cardiac Fibroblasts through Akt\/\u0391-SMA Pathway"],"prefix":"10.1016","volume":"17","author":[{"given":"Xiaomei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212000, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212000, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbo","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212000, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212000, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Health and Nursing, ZhenJiang College, Zhenjiang 212000, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aibin","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212000, Jiangsu, China | School of Health and Nursing, ZhenJiang College, Zhenjiang 212000, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","container-title":["Current Molecular Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.eurekaselect.com\/article\/download\/238247","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/238247\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/article\/download\/238247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T22:59:07Z","timestamp":1746572347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/238247\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":0,"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/0118761429353519250106115016","relation":{},"ISSN":["1874-4672"],"issn-type":[{"value":"1874-4672","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1,31]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2024-09-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-05","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication 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