{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:49:11Z","timestamp":1740142151339,"version":"3.37.3"},"reference-count":46,"publisher":"Bentham Science Publishers Ltd.","issue":"10","funder":[{"DOI":"10.13039\/501100008043","name":"Science Foundation of Zhejiang Sci-Tech University","doi-asserted-by":"publisher","award":["16042062-Y"],"award-info":[{"award-number":["16042062-Y"]}],"id":[{"id":"10.13039\/501100008043","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008990","name":"Key Research and Development Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2019C02043"],"award-info":[{"award-number":["2019C02043"]}],"id":[{"id":"10.13039\/501100008990","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010248","name":"Public Welfare Technology Application Research Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2017C32049"],"award-info":[{"award-number":["2017C32049"]}],"id":[{"id":"10.13039\/501100010248","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["PPL"],"published-print":{"date-parts":[[2019,9,30]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p>Porcine circovirus and Mycoplasma hyopneumoniae can cause respiratory diseases in pigs, which cause serious economic loss in the worldwide pig industry. Currently, these infections are mainly prevented and controlled by vaccination. The new vaccines on the market are mainly composed of subunits and inactivated vaccines but usually have lower antigenicity than traditional live vaccines. Thus, there is an increasing need to develop new adjuvants that can cause rapid and long-lasting immunity to enhance the antigenic efficacy for vaccines. Studies have shown that meningococcal porin PorB can act as a ligand to combine with Toll-like receptors to activate the production of immunological projections and act as a vaccine immunological adjuvant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective:<\/jats:title><jats:p>In this article, we expressed and purified the recombinant PorB protein and verified its immunogenicity against porcine circovirus type 2 and Mycoplasma hyopneumoniae genetically engineered vaccine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>In this article, we used prokaryotic expression to express and purify recombinant PorB protein, four different concentrations of PorB protein, Freund's adjuvant with two genetically engineered vaccines were combined with subcutaneous immunization of mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Our study shows that the appropriate dose of the recombinant protein PorB can enhance the levels of humoral and cellular responses induced by two genetically engineered vaccines in a short period of time in mice. The PorB adjuvant group may cause statistically higher antibody titers for both genetically engineered vaccines compared to Freund's commercial adjuvant (P&lt;0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>The recombinant protein PorB may be a good candidate adjuvant for improving the protective effect of vaccines against porcine circovirus type 2 and Mycoplasma hyopneumoniae, and the protein can be used for future practical applications.<\/jats:p><\/jats:sec>","DOI":"10.2174\/0929866526666190430115052","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T05:36:41Z","timestamp":1560836201000},"page":"776-784","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunoenhancement of Recombinant Neisseria meningitides PorB Protein on Porcine Circovirus Type 2 and Mycoplasma hyopneumoniae Genetically Engineered Vaccines"],"prefix":"10.2174","volume":"26","author":[{"given":"Rui","family":"Yang","sequence":"first","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Tao","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaojian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhong","family":"Shu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulong","family":"He","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.carbpol.2017.02.058","volume":"166","author":"Zhang G.","year":"2017","unstructured":"Zhang G.; Jia P.; Cheng G.; Jiao S.; Ren L.; Ji S.; Hu T.; Liu H.; Du Y.; Enhanced immune response to inactivated porcine circovirus type 2 (PCV2) vaccine by conjugation of chitosan oligosaccharides. Carbohydr Polym 2017,166,64-72","journal-title":"Carbohydr Polym"},{"key":"ref=2","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.virusres.2011.09.041","volume":"164","author":"Beach N.M.","year":"2012","unstructured":"Beach N.M.; Meng X.J.; Efficacy and future prospects of commercially available and experimental vaccines against porcine circovirus type 2 (PCV2). Virus Res 2012,164(1-2),33-42","journal-title":"Virus Res"},{"key":"ref=3","doi-asserted-by":"publisher","first-page":"5832","DOI":"10.1016\/j.vaccine.2014.08.008","volume":"32","author":"Virginio V.G.","year":"2014","unstructured":"Virginio V.G.; Gonchoroski T.; Paes J.A.; Schuck D.C.; Zaha A.; Ferreira H.B.; Immune responses elicited by Mycoplasma hyopneumoniae recombinant antigens and DNA constructs with potential for use in vaccination against porcine enzootic pneumonia. Vaccine 2014,32(44),5832-5838","journal-title":"Vaccine"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1128\/CVI.00415-06","volume":"14","author":"Okamba F.R.","year":"2007","unstructured":"Okamba F.R.; Moreau E.; Cheikh Saad Bouh K.; Gagnon C.A.; Massie B.; Arella M.; Immune responses induced by replication-defective adenovirus expressing the C-terminal portion of the Mycoplasma hyopneumoniae P97 adhesin. Clin Vaccine Immunol 2007,14(6),767-774","journal-title":"Clin Vaccine Immunol"},{"key":"ref=5","doi-asserted-by":"publisher","first-page":"2903","DOI":"10.1016\/j.vaccine.2015.04.065","volume":"33","author":"Galliher-Beckley A.","year":"2015","unstructured":"Galliher-Beckley A.; Pappan L.K.; Madera R.; Burakova Y.; Waters A.; Nickles M.; Li X.; Nietfeld J.; Schlup J.R.; Zhong Q.; McVey S.; Dritz S.S.; Shi J.; Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines. Vaccine 2015,33(25),2903-2908","journal-title":"Vaccine"},{"key":"ref=6","doi-asserted-by":"publisher","first-page":"3222","DOI":"10.4049\/jimmunol.179.5.3222","volume":"179","author":"Burke J.M.","year":"2007","unstructured":"Burke J.M.; Ganley-Leal L.M.; Khatri A.; Wetzler L.M.; Neisseria meningitidis PorB, a TLR2 ligand, induces an antigen-specific eosinophil recall response: potential adjuvant for helminth vaccines? J Immunol 2007,179(5),3222-3230","journal-title":"J Immunol"},{"key":"ref=7","doi-asserted-by":"publisher","first-page":"783","DOI":"10.3389\/fmicb.2018.00783","volume":"9","author":"Tan K.","year":"2018","unstructured":"Tan K.; Li R.; Huang X.; Liu Q.; Outer membrane vesicles: current status and future direction of these novel vaccine adjuvants. Front Microbiol 2018,9,783","journal-title":"Front Microbiol"},{"key":"ref=8","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1016\/S1359-6446(03)02864-2","volume":"8","author":"Marciani D.J.","year":"2003","unstructured":"Marciani D.J.; Vaccine adjuvants: role and mechanisms of action in vaccine immunogenicity. Drug Discov Today 2003,8(20),934-943","journal-title":"Drug Discov Today"},{"key":"ref=9","doi-asserted-by":"publisher","first-page":"2373","DOI":"10.4049\/jimmunol.176.4.2373","volume":"176","author":"Massari P.","year":"2006","unstructured":"Massari P.; Visintin A.; Gunawardana J.; Halmen K.A.; King C.A.; Golenbock D.T.; Wetzler L.M.; Meningococcal porin PorB binds to TLR2 and requires TLR1 for signaling. J Immunol 2006,176(4),2373-2380","journal-title":"J Immunol"},{"key":"ref=10","doi-asserted-by":"publisher","first-page":"7689","DOI":"10.1016\/j.vaccine.2018.10.074","volume":"36","author":"Mosaheb M.","year":"2018","unstructured":"Mosaheb M.; Wetzler L.M.; Meningococcal PorB induces a robust and diverse antigen specific T cell response as a vaccine adjuvant. Vaccine 2018,36(50),7689-7699","journal-title":"Vaccine"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"3417","DOI":"10.1128\/IAI.00683-12","volume":"80","author":"Toussi D.N.","year":"2012","unstructured":"Toussi D.N.; Carraway M.; Wetzler L.M.; Lewis L.A.; Liu X.; Massari P.; The amino acid sequence of Neisseria lactamica PorB surface-exposed loops influences Toll-like receptor 2-dependent cell activation. Infect Immun 2012,80(10),3417-3428","journal-title":"Infect Immun"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.jcis.2008.03.001","volume":"322","author":"Chamani J.","year":"2008","unstructured":"Chamani J.; Heshmati M.; Mechanism for stabilization of the molten globule state of papain by sodium n-alkyl sulfates: spectroscopic and calorimetric approaches. J Colloid Interface Sci 2008,322(1),119-127","journal-title":"J Colloid Interface Sci"},{"key":"ref=13","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1107\/S1744309109032333","volume":"65","author":"Tanabe M.","year":"2009","unstructured":"Tanabe M.; Iverson T.M.; Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB. Acta Crystallogr Sect F Struct Biol Cryst Commun 2009,65(Pt 10),996-1000","journal-title":"Acta Crystallogr Sect F Struct Biol Cryst Commun"},{"key":"ref=14","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1128\/IAI.62.6.2432-2439.1994","volume":"62","author":"Qi H.L.","year":"1994","unstructured":"Qi H.L.; Tai J.Y.; Blake M.S.; Expression of large amounts of neisserial porin proteins in Escherichia coli and refolding of the proteins into native trimers. Infect Immun 1994,62(6),2432-2439","journal-title":"Infect Immun"},{"key":"ref=15","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.molstruc.2010.06.035","volume":"979","author":"Chamani J.","year":"2010","unstructured":"Chamani J.; Energetic domains analysis of bovine \u03b1-lactalbumin upon interaction with copper and dodecyl trimethylammonium bromide. J Mol Struct 2010,979(1-3),227-234","journal-title":"J Mol Struct"},{"key":"ref=16","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.ijpharm.2015.08.048","volume":"495","author":"Nikpoor A.R.","year":"2015","unstructured":"Nikpoor A.R.; Tavakkol-Afshari J.; Gholizadeh Z.; Sadri K.; Babaei M.H.; Chamani J.; Badiee A.; Jalali S.A.; Jaafari M.R.; Nanoliposome-mediated targeting of antibodies to tumors: IVIG antibodies as a model. Int J Pharm 2015,495(1),162-170","journal-title":"Int J Pharm"},{"key":"ref=17","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.jchromb.2015.04.004","volume":"991","author":"Zaveckas M.","year":"2015","unstructured":"Zaveckas M.; Snipaitis S.; Pesliakas H.; Nainys J.; Gedvilaite A.; Purification of recombinant virus-like particles of porcine circovirus type 2 capsid protein using ion-exchange monolith chromatography. J Chromatogr B Analyt Technol Biomed Life Sci 2015,991,21-28","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"ref=18","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.vaccine.2012.10.088","volume":"31","author":"Galli V.","year":"2012","unstructured":"Galli V.; Simionatto S.; Marchioro S.B.; Fisch A.; Gomes C.K.; Concei\u00e7\u00e3o F.R.; Dellagostin O.A.; Immunisation of mice with Mycoplasma hyopneumoniae antigens P37, P42, P46 and P95 delivered as recombinant subunit or DNA vaccines. Vaccine 2012,31(1),135-140","journal-title":"Vaccine"},{"key":"ref=19","doi-asserted-by":"publisher","first-page":"3374","DOI":"10.1016\/j.vaccine.2005.12.068","volume":"24","author":"Wang X.","year":"2006","unstructured":"Wang X.; Jiang W.; Jiang P.; Li Y.; Feng Z.; Xu J.; Construction and immunogenicity of recombinant adenovirus expressing the capsid protein of porcine circovirus 2 (PCV2) in mice. Vaccine 2006,24(16),3374-3380","journal-title":"Vaccine"},{"key":"ref=20","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/1574-6968.12389","volume":"352","author":"Barate A.K.","year":"2014","unstructured":"Barate A.K.; Cho Y.; Truong Q.L.; Hahn T.W.; Immunogenicity of IMS 1113 plus soluble subunit and chimeric proteins containing Mycoplasma hyopneumoniae P97 C-terminal repeat regions. FEMS Microbiol Lett 2014,352(2),213-220","journal-title":"FEMS Microbiol Lett"},{"key":"ref=21","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S0264-410X(02)00443-7","volume":"21","author":"Bastos R.G.","year":"2002","unstructured":"Bastos R.G.; Dellagostin O.A.; Barletta R.G.; Doster A.R.; Nelson E.; Osorio F.A.; Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus. Vaccine 2002,21(1-2),21-29","journal-title":"Vaccine"},{"key":"ref=22","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.vetimm.2010.05.013","volume":"137","author":"Fort M.","year":"2010","unstructured":"Fort M.; Sibila M.; Nofrar\u00edas M.; P\u00e9rez-Mart\u00edn E.; Olvera A.; Mateu E.; Segal\u00e9s J.; Porcine circovirus type 2 (PCV2) Cap and Rep proteins are involved in the development of cell-mediated immunity upon PCV2 infection. Vet Immunol Immunopathol 2010,137(3-4),226-234","journal-title":"Vet Immunol Immunopathol"},{"key":"ref=23","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1016\/j.vaccine.2015.09.007","volume":"34","author":"Li P.C.","year":"2016","unstructured":"Li P.C.; Qiao X.W.; Zheng Q.S.; Hou J.B.; Immunogenicity and immunoprotection of porcine circovirus type 2 (PCV2) Cap protein displayed by Lactococcus lactis. Vaccine 2016,34(5),696-702","journal-title":"Vaccine"},{"key":"ref=24","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.rvsc.2017.12.004","volume":"117","author":"Chen Y.","year":"2018","unstructured":"Chen Y.; Song T.; Xiao Y.L.; Wan X.; Yang L.; Li J.; Zeng G.; Fang P.; Wang Z.Z.; Gao R.; Enhancement of immune response of piglets to PCV-2 vaccine by porcine IL-2 and fusion IL-4\/6 gene entrapped in chitosan nanoparticles. Res Vet Sci 2018,117,224-232","journal-title":"Res Vet Sci"},{"key":"ref=25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147432","volume":"11","author":"Zhu S.","year":"2016","unstructured":"Zhu S.; Zhang C.; Wang J.; Wei L.; Quan R.; Yang J.; Yan X.; Li Z.; She R.; Hu F.; Liu J.; Immunity elicited by an experimental vaccine based on recombinant flagellin-porcine Circovirus type 2 Cap fusion protein in piglets. PLoS One 2016,11(2)","journal-title":"PLoS One"},{"key":"ref=26","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.tvjl.2008.02.020","volume":"181","author":"Sibila M.","year":"2009","unstructured":"Sibila M.; Pieters M.; Molitor T.; Maes D.; Haesebrouck F.; Segal\u00e9s J.; Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection. Vet J 2009,181(3),221-231","journal-title":"Vet J"},{"key":"ref=27","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.vetmic.2007.09.008","volume":"126","author":"Maes D.","year":"2008","unstructured":"Maes D.; Segales J.; Meyns T.; Sibila M.; Pieters M.; Haesebrouck F.; Control of Mycoplasma hyopneumoniae infections in pigs. Vet Microbiol 2008,126(4),297-309","journal-title":"Vet Microbiol"},{"key":"ref=28","doi-asserted-by":"publisher","first-page":"5734","DOI":"10.1016\/j.vaccine.2006.04.036","volume":"24","author":"Concei\u00e7\u00e3o F.R.","year":"2006","unstructured":"Concei\u00e7\u00e3o F.R.; Moreira A.N.; Dellagostin O.A.; A recombinant chimera composed of R1 repeat region of Mycoplasma hyopneumoniae P97 adhesin with Escherichia coli heat-labile enterotoxin B subunit elicits immune response in mice. Vaccine 2006,24(29-30),5734-5743","journal-title":"Vaccine"},{"key":"ref=29","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.vetmic.2013.04.019","volume":"165","author":"Simionatto S.","year":"2013","unstructured":"Simionatto S.; Marchioro S.B.; Maes D.; Dellagostin O.A.; Mycoplasma hyopneumoniae: From disease to vaccine development. Vet Microbiol 2013,165(3-4),234-242","journal-title":"Vet Microbiol"},{"key":"ref=30","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.vetmic.2018.04.005","volume":"219","author":"Garza-Moreno L.","year":"2018","unstructured":"Garza-Moreno L.; Segal\u00e9s J.; Pieters M.; Romagosa A.; Sibila M.; Acclimation strategies in gilts to control Mycoplasma hyopneumoniae infection. Vet Microbiol 2018,219,23-29","journal-title":"Vet Microbiol"},{"key":"ref=31","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1016\/j.ijpharm.2008.04.036","volume":"364","author":"Perrie Y.","year":"2008","unstructured":"Perrie Y.; Mohammed A.R.; Kirby D.J.; McNeil S.E.; Bramwell V.W.; Vaccine adjuvant systems: enhancing the efficacy of sub-unit protein antigens. Int J Pharm 2008,364(2),272-280","journal-title":"Int J Pharm"},{"key":"ref=32","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1038\/15058","volume":"17","author":"Singh M.","year":"1999","unstructured":"Singh M.; O\u2019Hagan D.; Advances in vaccine adjuvants. Nat Biotechnol 1999,17(11),1075-1081","journal-title":"Nat Biotechnol"},{"key":"ref=33","doi-asserted-by":"publisher","first-page":"5199","DOI":"10.1016\/j.vaccine.2007.04.089","volume":"25","author":"Cheng G.","year":"2007","unstructured":"Cheng G.; Zhao X.; Yan W.; Wang W.; Zuo X.; Huang K.; Liu Y.; Chen J.; Wang J.; Cong W.; Liu M.; Gao H.; Chen J.; Lu Y.; Zheng Z.; Alpha interferon is a powerful adjuvant for a recombinant protein vaccine against foot-and-mouth disease virus in swine, and an effective stimulus of in vivo immune response. Vaccine 2007,25(28),5199-5208","journal-title":"Vaccine"},{"key":"ref=34","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1128\/CMR.13.1.144","volume":"13","author":"van Deuren M.","year":"2000","unstructured":"van Deuren M.; Brandtzaeg P.; van der Meer J.W.; Update on meningococcal disease with emphasis on pathogenesis and clinical management. Clin Microbiol Rev 2000,13(1),144-166","journal-title":"Clin Microbiol Rev"},{"key":"ref=35","doi-asserted-by":"publisher","first-page":"2702","DOI":"10.1016\/S0264-410X(99)00011-0","volume":"17","author":"Peeters C.C.","year":"1999","unstructured":"Peeters C.C.; Claassen I.J.; Schuller M.; Kersten G.F.; van der Voort E.M.; Poolman J.T.; Immunogenicity of various presentation forms of PorA outer membrane protein of Neisseria meningitidis in mice. Vaccine 1999,17(20-21),2702-2712","journal-title":"Vaccine"},{"key":"ref=36","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S0966-842X(02)00037-9","volume":"11","author":"Massari P.","year":"2003","unstructured":"Massari P.; Ram S.; Macleod H.; Wetzler L.M.; The role of porins in neisserial pathogenesis and immunity. Trends Microbiol 2003,11(2),87-93","journal-title":"Trends Microbiol"},{"key":"ref=37","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.pep.2005.04.021","volume":"44","author":"Massari P.","year":"2005","unstructured":"Massari P.; King C.A.; MacLeod H.; Wetzler L.M.; Improved purification of native meningococcal porin PorB and studies on its structure\/function. Protein Expr Purif 2005,44(2),136-146","journal-title":"Protein Expr Purif"},{"key":"ref=38","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/s41598-017-00555-4","volume":"7","author":"Reiser M.L.","year":"2017","unstructured":"Reiser M.L.; Mosaheb M.M.; Lisk C.; Platt A.; Wetzler L.M.; The TLR2 binding Neisserial porin PorB enhances antigen presenting cell trafficking and cross-presentation. Sci Rep 2017,7(1),736","journal-title":"Sci Rep"},{"key":"ref=39","doi-asserted-by":"publisher","first-page":"3545","DOI":"10.4049\/jimmunol.174.6.3545","volume":"174","author":"Singleton T.E.","year":"2005","unstructured":"Singleton T.E.; Massari P.; Wetzler L.M.; Neisserial porin-induced dendritic cell activation is MyD88 and TLR2 dependent. J Immunol 2005,174(6),3545-3550","journal-title":"J Immunol"},{"key":"ref=40","doi-asserted-by":"publisher","first-page":"749","DOI":"10.2217\/fmb.10.41","volume":"5","author":"Wetzler L.M.","year":"2010","unstructured":"Wetzler L.M.; Innate immune function of the neisserial porins and the relationship to vaccine adjuvant activity. Future Microbiol 2010,5(5),749-758","journal-title":"Future Microbiol"},{"key":"ref=41","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1084\/jem.183.3.1151","volume":"183","author":"Wetzler L.M.","year":"1996","unstructured":"Wetzler L.M.; Ho Y.; Reiser H.; Neisserial porins induce B lymphocytes to express costimulatory B7-2 molecules and to proliferate. J Exp Med 1996,183(3),1151-1159","journal-title":"J Exp Med"},{"key":"ref=42","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1016\/j.vaccine.2007.11.080","volume":"26","author":"Liu X.","year":"2008","unstructured":"Liu X.; Wetzler L.M.; Massari P.; The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant. Vaccine 2008,26(6),786-796","journal-title":"Vaccine"},{"key":"ref=43","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1126\/science.2452484","volume":"240","author":"Lowell G.H.","year":"1988","unstructured":"Lowell G.H.; Ballou W.R.; Smith L.F.; Wirtz R.A.; Zollinger W.D.; Hockmeyer W.T.; Proteosome-lipopeptide vaccines: enhancement of immunogenicity for malaria CS peptides. Science 1988,240(4853),800-802","journal-title":"Science"},{"key":"ref=44","doi-asserted-by":"publisher","first-page":"59","DOI":"10.3892\/ijo.15.1.59","volume":"15","author":"Alonso D.F.","year":"1999","unstructured":"Alonso D.F.; Gabri M.R.; Guthmann M.D.; Fainboim L.; Gomez D.E.; A novel hydrophobized GM3 ganglioside\/Neisseria meningitidis outer-membrane-protein complex vaccine induces tumor protection in B16 murine melanoma. Int J Oncol 1999,15(1),59-66","journal-title":"Int J Oncol"},{"key":"ref=45","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1111\/j.1749-6632.1994.tb44295.x","volume":"730","author":"Wetzler L.M.","year":"1994","unstructured":"Wetzler L.M.; Immunopotentiating ability of neisserial major outer membrane proteins. Use as an adjuvant for poorly immunogenic substances and potential use in vaccines. Ann N Y Acad Sci 1994,730,367-370","journal-title":"Ann N Y Acad Sci"},{"key":"ref=46","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.4049\/jimmunol.145.9.3071","volume":"145","author":"Donnelly J.J.","year":"1990","unstructured":"Donnelly J.J.; Deck R.R.; Liu M.A.; Immunogenicity of a Haemophilus influenzae polysaccharide-Neisseria meningitidis outer membrane protein complex conjugate vaccine. J Immunol 1990,145(9),3071-3079","journal-title":"J Immunol"}],"container-title":["Protein &amp; Peptide Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/171915","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T14:33:14Z","timestamp":1694961194000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/171915\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,30]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,9,30]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/0929866526666190430115052","relation":{},"ISSN":["0929-8665"],"issn-type":[{"type":"print","value":"0929-8665"}],"subject":[],"published":{"date-parts":[[2019,9,30]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-08","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}