{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T03:30:09Z","timestamp":1764646209283},"reference-count":35,"publisher":"Bentham Science Publishers Ltd.","issue":"12","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["PPL"],"published-print":{"date-parts":[[2019,10,11]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Melanin plays a crucial role in camouflage, social communication and\nprotection against harmful ultraviolet radiations. Melanin is synthesized by melanocytes through\nmelanogenesis and several intrinsic and extrinsic factors are involved during the process. Any\nchange occuring in the normal melanogenesis process can cause severe pigmentation problems of\nhypopigmentation or hyperpigmentation.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>The present study is based on the evaluation of the effect of thymoquinone on\nmelanogenesis and their possible mechanism of action using the B16F10 melanoma cell line for the\nproduction via blocking signaling pathways.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Phase contrast microscopy, cell viability, tyrosinase activity, melanin content and\nwestern blot analysis were used in the present study.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>In the present investigation, cultured melanocytes exhibit that the stimulation of melanin\nsynthesis when treated with thymoquinone. Tyrosinase activity and melanin production in B16F10\nmelanoma cell line was increased in doze-dependent manner. In western blot, we investigated the\ninvolvement of the cAMP\/PKA pathway in thymoquinone induced melanogenesis. It was observed\nprotein kinase inhibitors PKA, PKC, PKB and MEK1 decreased the stimulatory effects of\nthymoquinone from 11.45- fold value to 8.312, 6.631, 4.51, and 7.211-fold value, respectively.\nHowever, the results also prove that thymoquinone may partially induce tyrosinase expression via\nPKA, PKB, PKC and MEK1 signaling pathways.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>The present finding proposed that thymoquinone is a protective challenger for\nmelanogenesis and it might be useful for the treatment of hypopigmentary disorders.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/0929866526666190506114604","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T07:36:13Z","timestamp":1557214573000},"page":"910-918","update-policy":"http:\/\/dx.doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Insight into Mechanistic Action of Thymoquinone Induced Melanogenesis in Cultured Melanocytes"],"prefix":"10.2174","volume":"26","author":[{"given":"Kamal U.","family":"Zaidi","sequence":"first","affiliation":[{"name":"Biotechnology Pharmacology Laboratory, Centre for Scientific Research and Development, People\u2019s University and Saifia Science College, Bhaopal-462001,India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firoz N.","family":"Khan","sequence":"additional","affiliation":[{"name":"Biotechnology Pharmacology Laboratory, Centre for Scientific Research and Development, People\u2019s University and Saifia Science College, Bhaopal-462001,India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharique A.","family":"Ali","sequence":"additional","affiliation":[{"name":"Biotechnology Pharmacology Laboratory, Centre for Scientific Research and Development, People\u2019s University and Saifia Science College, Bhaopal-462001,India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kausar P.","family":"Khan","sequence":"additional","affiliation":[{"name":"Biotechnology Pharmacology Laboratory, Centre for Scientific Research and Development, People\u2019s University and Saifia Science College, Bhaopal-462001,India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1038\/nature05660","volume":"445","author":"Lin J.Y.","year":"2007","unstructured":"Lin J.Y.; Fisher D.E.; Melanocyte biology and skin pigmentation. 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