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Moreover, we assessed the role of +36 GFP for eliciting HPV-related anti-tumor effects in mice due to the lack of HPV replication in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Our data showed that +36 GFP efficiently enter the cells and augment the transfection rate of HPV16E7 antigen, as well. Furthermore, +36 GFP significantly reduced HCV, HIV and HSV replication up to 75%, 49% and 43% in HCV-infected Huh7.5 cells, HIV-infected Hela cells and HSV-infected Vero cells, respectively. On the other hand, mice immunization with +36 GFP complexed with HPV16 E7 antigen (+36GFP + E7) or fused to HPV16 E7 antigen (+36GFP-E7) elicited a higher Th1 cellular immune response with the predominant IgG2a, IgG2b, IFN-\u03b3 and Granzyme B levels than those induced by other groups. These regimens protected mice against TC- 1 tumor challenge (~ 67%) compared to E7 protein alone (~ 33%). These data suggested that +36 GFP can act as an anti-viral agent at certain dose due to its high efficiency in cell penetration in vitro and in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>Generally, +36 GFP targets viral replication in vitro as well as helps to suppress the growth of HPV-related tumors in vivo.<\/jats:p><\/jats:sec>","DOI":"10.2174\/0929866526666190823145916","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T06:10:48Z","timestamp":1566799848000},"page":"930-939","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti-viral Effects of Superpositively Charged Mutant of Green Fluorescent Protein"],"prefix":"10.2174","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3102-553X","authenticated-orcid":true,"given":"Rouhollah","family":"Vahabpour","sequence":"first","affiliation":[{"name":"Department of Medical Lab Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences; Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parya","family":"Basimi","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzin","family":"Roohvand","sequence":"additional","affiliation":[{"name":"Department of Virology, Pasteur Institute of Iran, Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan","family":"Asadi","sequence":"additional","affiliation":[{"name":"Deputy of Research, Technology and Education, Research Section, Pasteur Institute of Iran, Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholnaz M.","family":"Irani","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rezvan","family":"Zabihollahi","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7363-7406","authenticated-orcid":true,"given":"Azam","family":"Bolhassani","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran,Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"11052","DOI":"10.1073\/pnas.1704937114","volume":"114","author":"Ganim Z.","year":"2017","unstructured":"Ganim Z.; Rief M.; Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding. 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