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Furthermore, the different concentrations of REG induce\nslight changes in the viscosity of ct-DNA. Zeta potential parameters indicated that hydrophobic interaction\nplays a major role in the DNA-REG complex. Results obtained from molecular docking\ndemonstrate that the REG prefers to bind on the minor groove of DNAs than that of the major\ngroove. Binding properties of HSA reveal that intrinsic fluorescence of HSA could be quenched by\nREG in a static mode. The competitive experiments in the presence of warfarin and ibuprofen (as\nsite markers) suggested that the binding site of REG to HSA was most probably located in the subdomain\nIIA. Measurements of the zeta potential indicated that REG bound to HSA mainly by both\nelectrostatic and hydrophobic interactions. It was found on docking procedures that REG could fit\nwell into HSA subdomain IIA, which confirmed the experimental results.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>In conclusion, REG can be delivered by HSA in a circulatory system and affect DNA\nas potential target.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/0929866527666200921164536","type":"journal-article","created":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T07:33:15Z","timestamp":1601623995000},"page":"290-303","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Use of Molecular Docking and Spectroscopic Methods for Investigation of The Interaction Between Regorafenib with Human Serum Albumin (HSA) and Calf Thymus DNA (Ct-DNA) In The Presence Of Different Site Markers"],"prefix":"10.2174","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1711-5619","authenticated-orcid":true,"given":"Hamid","family":"Tanzadehpanah","sequence":"first","affiliation":[{"name":"Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1810-9966","authenticated-orcid":true,"given":"Hanie","family":"Mahaki","sequence":"additional","affiliation":[{"name":"Vascular and Endovascular Surgery Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1398-8527","authenticated-orcid":true,"given":"Mohammadreza","family":"Moradi","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeid","family":"Afshar","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neda Hosseinpour","family":"Moghadam","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadegh","family":"Salehzadeh","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1326-1288","authenticated-orcid":true,"given":"Rezvan","family":"Najafi","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Razieh","family":"Amini","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8910-556X","authenticated-orcid":true,"given":"Massoud","family":"Saidijam","sequence":"additional","affiliation":[{"name":"Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1177\/1060028013509792","volume":"47","author":"Crona D.J.","year":"2013","unstructured":"Crona D.J.; Keisler M.D.; Walko C.M.; Regorafenib: a novel multitargeted tyrosine kinase inhibitor for colorectal cancer and gastrointestinal stromal tumors. 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