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In our previous work,\nwe inserted an additional Cys in K-Ras4B HVR peptide synthesis for NCL in the semi-synthesis of\nK-Ras4b protein, but it is not suitable for application in protein dimerization research. The recently\ndeveloped selenocysteine (Sec, U) mediated native chemical ligation reaction followed by selective\ndeselenization, which can help to broaden the scope of protein synthesis, requires the generation of\nthe peptide fragment with an N-terminal Sec.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>To synthesize K-Ras4B HVR peptide containing both N-terminal Sec and C-terminal farnesylated and\nmethylated Cys to achieve traceless protein semi-synthesis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods and Results:<\/jats:title>\n<jats:p>We have developed a facile synthesis approach for producing Boc-Sec)2-OH\nusing economic Se powder, which can facilitate scaling up preparation of peptides containing Sec\nat the N-terminus. Furthermore, we synthesized K-Ras4B HVR peptide containing selenocystine\nby utilization of Boc-Sec)2-OH. Finally, we took K-Ras4B HVR peptide as an example to test the\ncompatibility of farnesylation reaction at Cys with the N-terminal Sec)2, and the farnesyl group\nwas successfully added to the thiol group of Cys.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/0929866527666201223094249","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T06:18:39Z","timestamp":1608790719000},"page":"603-611","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Facile Synthesis of Boc-Protected Selenocystine and its Compatibility with Late-Stage Farnesylation at Cysteine Site"],"prefix":"10.2174","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7127-4920","authenticated-orcid":true,"given":"Peng-Cheng","family":"Zhu","sequence":"first","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3518-0139","authenticated-orcid":true,"given":"Yong-Xiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1146\/annurev.bi.65.070196.000503","volume":"65","author":"Stadtman T.C.","year":"1996","unstructured":"Stadtman T.C.; Selenocysteine. 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