{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:50:52Z","timestamp":1766087452572,"version":"3.37.3"},"reference-count":32,"publisher":"Bentham Science Publishers Ltd.","issue":"27","funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20160343","BK20150299"],"award-info":[{"award-number":["BK20160343","BK20150299"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601992","81601865"],"award-info":[{"award-number":["81601992","81601865"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPD"],"published-print":{"date-parts":[[2019,10,9]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p> Osteoarthritis (OA) is a common joint disease characterized by cartilage degeneration.\nLong non-coding RNAs (lncRNAs) have been associated with inflammatory diseases, including OA. Here, we\ninvestigated the potential molecular role of lncRNAs in OA pathogenesis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>ATDC5 cells were treated with lipopolysaccharides (LPS), and qPCR was used to identify and determine\nexpression of potential lncRNAs involved in LPS-induced chondrocyte injury. Cell viability, apoptosis, and expression\nof cartilage-related genes and inflammatory cytokines were assessed after CTD-2574D22.4 knockdown.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>After LPS stimulation, CTD-2574D22.4 was found to be the second highest up-regulated gene, and the\nenhanced expression was validated in OA chondrocytes. Moreover, CTD-2574D22.4 inhibition significantly rescued\ncell viability, suppressed by LPS stress, and markedly attenuated LPS-induced apoptosis. The expression of\ncartilage-degrading enzymes MMP-13 and ADAMTS-5 were increased, while type II collagen was reduced after\nLPS treatment. This trend was largely reversed by CTD-2574D22.4 knockdown. Additionally, mRNA and protein\nlevels of key inflammatory cytokines (TNF-a, IL-6, and IL-1\u03b2) were significantly elevated in the LPS group and\npartially relieved upon CTD-2574D22.4 knockdown.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>CTD2574D22.4 knockdown ameliorates LPS-induced cartilage injury by protecting chondrocytes\nfrom apoptosis via anti-inflammation and anti- cartilage-degrading pathways. Thus, CTD2574D22.4 might be a\npotential diagnostic and therapeutic target for OA.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1381612825666190801141801","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T10:45:37Z","timestamp":1564656337000},"page":"2969-2974","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Inhibition of Long Non-coding RNA CTD-2574D22.4 Alleviates LPS-induced Apoptosis and Inflammatory Injury of Chondrocytes"],"prefix":"10.2174","volume":"25","author":[{"given":"Lisong","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No.188 Shizi Road, Suzhou 215006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No.188 Shizi Road, Suzhou 215006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No.188 Shizi Road, Suzhou 215006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinghua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No.188 Shizi Road, Suzhou 215006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No.188 Shizi Road, Suzhou 215006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center of Reproduction and Genetics, Suzhou Municipal Hospital, Affiliated Suzhou Hospital of Nanjing Medical University, 26 Daoqian Road, Suzhou, Jiangsu 215002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University, No.188 Shizi Road, Suzhou 215006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","first-page":"1705","volume":"23","author":"Okubo M.","year":"2013","unstructured":"Okubo M.; Okada Y.; Destruction of the articular cartilage in osteoarthritis. Clin Calcium 2013,23(12),1705-1713","journal-title":"Clin Calcium"},{"key":"ref=2","doi-asserted-by":"publisher","first-page":"2115","DOI":"10.1016\/S0140-6736(11)60243-2","volume":"377","author":"Bijlsma J.W.","year":"2011","unstructured":"Bijlsma J.W.; Berenbaum F.; Lafeber F.P.; Osteoarthritis: an update with relevance for clinical practice. Lancet 2011,377(9783),2115-2126","journal-title":"Lancet"},{"key":"ref=3","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.1002\/art.34453","volume":"64","author":"Loeser R.F.","year":"2012","unstructured":"Loeser R.F.; Goldring S.R.; Scanzello C.R.; Goldring M.B.; Osteoarthritis: a disease of the joint as an organ. Arthritis Rheum 2012,64(6),1697-1707","journal-title":"Arthritis Rheum"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1136\/annrheumdis-2013-204763","volume":"73","author":"Cross M.","year":"2014","unstructured":"Cross M.; Smith E.; Hoy D.; The global burden of hip and knee osteoarthritis: estimates from the global burden of disease 2010 study. Ann Rheum Dis 2014,73(7),1323-1330","journal-title":"Ann Rheum Dis"},{"key":"ref=5","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1136\/annrheumdis-2013-204905","volume":"74","author":"Schnitzer T.J.","year":"2015","unstructured":"Schnitzer T.J.; Ekman E.F.; Spierings E.L.; Efficacy and safety of tanezumab monotherapy or combined with non-steroidal anti-inflammatory drugs in the treatment of knee or hip osteoarthritis pain. Ann Rheum Dis 2015,74(6),1202-1211","journal-title":"Ann Rheum Dis"},{"key":"ref=6","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1016\/j.joca.2012.07.010","volume":"20","author":"van der Kraan P.M.","year":"2012","unstructured":"van der Kraan P.M.; Osteoarthritis year 2012 in review: biology. Osteoarthritis Cartilage 2012,20(12),1447-1450","journal-title":"Osteoarthritis Cartilage"},{"key":"ref=7","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.cell.2009.02.006","volume":"136","author":"Ponting C.P.","year":"2009","unstructured":"Ponting C.P.; Oliver P.L.; Reik W.; Evolution and functions of long noncoding RNAs. Cell 2009,136(4),629-641","journal-title":"Cell"},{"key":"ref=8","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1038\/nrg2521","volume":"10","author":"Mercer T.R.","year":"2009","unstructured":"Mercer T.R.; Dinger M.E.; Mattick J.S.; Long non-coding RNAs: insights into functions. Nat Rev Genet 2009,10(3),155-159","journal-title":"Nat Rev Genet"},{"key":"ref=9","doi-asserted-by":"publisher","first-page":"3595","DOI":"10.1096\/fj.14-260323","volume":"29","author":"Marques-Rocha J.L.","year":"2015","unstructured":"Marques-Rocha J.L.; Samblas M.; Milagro F.I.; Bressan J.; Mart\u00ednez J.A.; Marti A.; Noncoding RNAs, cytokines, and inflammation-related diseases. FASEB J 2015,29(9),3595-3611","journal-title":"FASEB J"},{"key":"ref=10","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.jbspin.2016.09.006","volume":"84","author":"Jiang S.D.","year":"2017","unstructured":"Jiang S.D.; Lu J.; Deng Z.H.; Li Y.S.; Lei G.H.; Long noncoding RNAs in osteoarthritis. Joint Bone Spine 2017,84(5),553-556","journal-title":"Joint Bone Spine"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1111\/os.12147","volume":"6","author":"Xing D.","year":"2014","unstructured":"Xing D.; Liang J.Q.; Li Y.; Identification of long noncoding RNA associated with osteoarthritis in humans. Orthop Surg 2014,6(4),288-293","journal-title":"Orthop Surg"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.lfs.2018.12.020","volume":"217","author":"Li X.","year":"2019","unstructured":"Li X.; Yu M.; Chen L.; LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203. Life Sci 2019,217,283-292","journal-title":"Life Sci"},{"key":"ref=13","doi-asserted-by":"publisher","first-page":"1241","DOI":"10.1159\/000487455","volume":"45","author":"Li F.","year":"2018","unstructured":"Li F.; Sun J.; Huang S.; Su G.; Pi G.; LncRNA GAS5 overexpression reverses LPS-induced inflammatory injury and apoptosis through up-regulating KLF2 expression in ATDC5 chondrocytes. Cell Physiol Biochem 2018,45(3),1241-1251","journal-title":"Cell Physiol Biochem"},{"key":"ref=14","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1038\/s41419-018-0746-z","volume":"9","author":"Hu J.","year":"2018","unstructured":"Hu J.; Wang Z.; Shan Y.; Pan Y.; Ma J.; Jia L.; Long non-coding RNA HOTAIR promotes osteoarthritis progression via miR-17-5p\/FUT2\/\u03b2-catenin axis. Cell Death Dis 2018,9(7),711","journal-title":"Cell Death Dis"},{"key":"ref=15","doi-asserted-by":"publisher","first-page":"381","DOI":"10.3349\/ymj.2019.60.4.381","volume":"60","author":"Hu Y.","year":"2019","unstructured":"Hu Y.; Li S.; Zou Y.; Knockdown of LncRNA H19 relieves LPS-induced damage by modulating miR-130a in osteoarthritis. Yonsei Med J 2019,60(4),381-388","journal-title":"Yonsei Med J"},{"key":"ref=16","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.gene.2016.04.016","volume":"586","author":"Zhang C.","year":"2016","unstructured":"Zhang C.; Wang P.; Jiang P.; Upregulation of lncRNA HOTAIR contributes to IL-1\u03b2-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis. Gene 2016,586(2),248-253","journal-title":"Gene"},{"key":"ref=17","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1016\/j.biopha.2018.07.048","volume":"106","author":"Cao L.","year":"2018","unstructured":"Cao L.; Wang Y.; Wang Q.; Huang J.; LncRNA FOXD2-AS1 regulates chondrocyte proliferation in osteoarthritis by acting as a sponge of miR-206 to modulate CCND1 expression. Biomed Pharmacother 2018,106,1220-1226","journal-title":"Biomed Pharmacother"},{"key":"ref=18","author":"Lu Z.","year":"2018","unstructured":"Lu Z.; Luo M.; Huang Y.; lncRNA-CIR regulates cell apoptosis of chondrocytes in osteoarthritis. J Cell Biochem In press2018","journal-title":"J Cell Biochem"},{"key":"ref=19","first-page":"8574","volume":"22","author":"Tang L.P.","year":"2018","unstructured":"Tang L.P.; Ding J.B.; Liu Z.H.; Zhou G.J.; LncRNA TUG1 promotes osteoarthritis-induced degradation of chondrocyte extracellular matrix via miR-195\/MMP-13 axis. Eur Rev Med Pharmacol Sci 2018,22(24),8574-8581","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"ref=20","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1080\/08869634.2017.1315511","volume":"36","author":"Perotto J.H.","year":"2018","unstructured":"Perotto J.H.; Camejo F.A.; Doetzer A.D.; Expression of MMP-13 in human temporomandibular joint disc derangement and osteoarthritis. Cranio 2018,36(3),161-166","journal-title":"Cranio"},{"key":"ref=21","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/759028","volume":"2014","author":"Chen Y.T.","year":"2014","unstructured":"Chen Y.T.; Hou C.H.; Hou S.M.; Liu J.F.; The effects of amphiregulin induced MMP-13 production in human osteoarthritis synovial fibroblast. Mediators Inflamm 2014,2014","journal-title":"Mediators Inflamm"},{"key":"ref=22","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1016\/j.joca.2014.04.006","volume":"22","author":"Lim N.H.","year":"2014","unstructured":"Lim N.H.; Meinjohanns E.; Meldal M.; Bou-Gharios G.; Nagase H.; \n                        In vivo imaging of MMP-13 activity in the murine destabilised medial meniscus surgical model of osteoarthritis. Osteoarthritis Cartilage 2014,22(6),862-868","journal-title":"Osteoarthritis Cartilage"},{"key":"ref=23","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.jmgm.2013.06.005","volume":"44","author":"Kalva S.","year":"2013","unstructured":"Kalva S.; Saranyah K.; Suganya P.R.; Nisha M.; Saleena L.M.; Potent inhibitors precise to S1\u2032 loop of MMP-13, a crucial target for osteoarthritis. J Mol Graph Model 2013,44,297-310","journal-title":"J Mol Graph Model"},{"key":"ref=24","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1007\/s00109-016-1418-z","volume":"94","author":"Ji Q.","year":"2016","unstructured":"Ji Q.; Xu X.; Zhang Q.; The IL-1\u03b2\/AP-1\/miR-30a\/ADAMTS-5 axis regulates cartilage matrix degradation in human osteoarthritis. J Mol Med (Berl) 2016,94(7),771-785","journal-title":"J Mol Med (Berl)"},{"key":"ref=25","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.joca.2015.09.005","volume":"24","author":"Miller R.E.","year":"2016","unstructured":"Miller R.E.; Tran P.B.; Ishihara S.; Larkin J.; Malfait A.M.; Therapeutic effects of an anti-ADAMTS-5 antibody on joint damage and mechanical allodynia in a murine model of osteoarthritis. Osteoarthritis Cartilage 2016,24(2),299-306","journal-title":"Osteoarthritis Cartilage"},{"key":"ref=26","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.archoralbio.2014.02.016","volume":"59","author":"Li W.","year":"2014","unstructured":"Li W.; Wu M.; Jiang S.; Ding W.; Luo Q.; Shi J.; Expression of ADAMTs-5 and TIMP-3 in the condylar cartilage of rats induced by experimentally created osteoarthritis. Arch Oral Biol 2014,59(5),524-529","journal-title":"Arch Oral Biol"},{"key":"ref=27","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1080\/03008207.2016.1208655","volume":"58","author":"Shen J.","year":"2017","unstructured":"Shen J.; Abu-Amer Y.; O\u2019Keefe R.J.; McAlinden A.; Inflammation and epigenetic regulation in osteoarthritis. Connect Tissue Res 2017,58(1),49-63","journal-title":"Connect Tissue Res"},{"key":"ref=28","doi-asserted-by":"publisher","first-page":"iii75","DOI":"10.1136\/ard.2008.098764","volume":"67","author":"Goldring M.B.","year":"2008","unstructured":"Goldring M.B.; Otero M.; Tsuchimochi K.; Ijiri K.; Li Y.; Defining the roles of inflammatory and anabolic cytokines in cartilage metabolism. Ann Rheum Dis 2008,67(Suppl. 3),iii75-iii82","journal-title":"Ann Rheum Dis"},{"key":"ref=29","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1002\/jor.23471","volume":"35","author":"Scanzello C.R.","year":"2017","unstructured":"Scanzello C.R.; Chemokines and inflammation in osteoarthritis: Insights from patients and animal models. J Orthop Res 2017,35(4),735-739","journal-title":"J Orthop Res"},{"key":"ref=30","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/728610","volume":"2015","author":"Jilani A.A.","year":"2015","unstructured":"Jilani A.A.; Mackworth-Young C.G.; The role of citrullinated protein antibodies in predicting erosive disease in rheumatoid arthritis: a systematic literature review and meta-analysis. Int J Rheumatol 2015,2015","journal-title":"Int J Rheumatol"},{"key":"ref=31","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/nrrheum.2010.196","volume":"7","author":"Kapoor M.","year":"2011","unstructured":"Kapoor M.; Martel-Pelletier J.; Lajeunesse D.; Pelletier J.P.; Fahmi H.; Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 2011,7(1),33-42","journal-title":"Nat Rev Rheumatol"},{"key":"ref=32","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1016\/j.biopha.2018.04.085","volume":"103","author":"Liang Z.","year":"2018","unstructured":"Liang Z.; Ren C.; Emodin attenuates apoptosis and inflammation induced by LPS through up-regulating lncRNA TUG1 in murine chondrogenic ATDC5 cells. Biomed Pharmacother 2018,103,897-902","journal-title":"Biomed Pharmacother"}],"container-title":["Current Pharmaceutical Design"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/174039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T05:58:07Z","timestamp":1575611887000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/174039\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,9]]},"references-count":32,"journal-issue":{"issue":"27","published-print":{"date-parts":[[2019,10,9]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1381612825666190801141801","relation":{},"ISSN":["1381-6128"],"issn-type":[{"type":"print","value":"1381-6128"}],"subject":[],"published":{"date-parts":[[2019,10,9]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}