{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T21:00:41Z","timestamp":1754600441657,"version":"3.37.3"},"reference-count":25,"publisher":"Bentham Science Publishers Ltd.","issue":"7","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81373649"],"award-info":[{"award-number":["81373649"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CCHTS"],"published-print":{"date-parts":[[2020,10,5]]},"abstract":"<jats:sec>\n<jats:title>Aim and Objective:<\/jats:title>\n<jats:p>Lupus nephritis (LN) is one of the major complications of systemic\nlupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission\nprocesses in LN have not been clarified but is of great need in the clinic. Using isobaric tags for\nrelative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in\nmice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using\nintraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody\nenzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is\ncollected and processed for iTRAQ to screen out functional proteins closely related to the onset\nand development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction)\nwere used for verification.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>We identified 136 proteins that marked quantitative information. Among them, Hp,\nIgkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx\nwere screened out for a close relationship with the pathogenesis and development of LN, which is\nworth further study.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1386207323666200416151836","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T11:31:01Z","timestamp":1587036661000},"page":"649-657","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology"],"prefix":"10.2174","volume":"23","author":[{"given":"Dong-Jiang","family":"Liao","sequence":"first","affiliation":[{"name":"Dermatology Department of the First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi-Ping","family":"Cheng","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang-Li","family":"Liang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Fan","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sui-Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Qian","family":"Hu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Fan","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan-Sheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1016\/j.autrev.2018.03.013","volume":"17","author":"Dumestre-P\u00e9rard C.","year":"2018","unstructured":"Dumestre-P\u00e9rard C.; Clavarino G.; Colliard S.; Cesbron J.Y.; Thielens N.M.; Antibodies targeting circulating protective molecules in lupus nephritis: Interest as serological biomarkers. 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