{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T09:07:23Z","timestamp":1793696843996,"version":"4.5.1"},"reference-count":12,"publisher":"Bentham Science Publishers Ltd.","issue":"10","funder":[{"DOI":"10.13039\/501100012272","name":"Economic and Technological Development in Longgang District of Shenzhen City","doi-asserted-by":"publisher","award":["LGKCYLWS2018000194"],"award-info":[{"award-number":["LGKCYLWS2018000194"]}],"id":[{"id":"10.13039\/501100012272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CDM"],"published-print":{"date-parts":[[2019,12,12]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>To investigate MiRNA-126 amounts in serum exosomes from allergic asthma patients as\nwell as lung tissues of asthmatic mice, evaluating the expression of its target gene DNMT1 in mouse specimens.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> MiRNA-126 amounts in serum exosomes from asthmatic patients were detected by real-time PCR. The\nmouse model of allergic asthma was established by OVA-sensitization, and allergic symptoms were recorded; serum\nIL-4 and sIgE level evaluation (ELISA), broncho alveolar lavage fluid (BALF) cell count and H&amp;E staining were\nperformed to assess airway inflammation. MiRNA-126 and DNMT1 levels in the lung of asthmatic and control mice\nwere detected by real-time PCR; DNMT1 protein levels were detected by immunoblot.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>MiRNA-126 amounts in peripheral blood exosomes from patients with allergic asthma were significantly\nhigher than that of healthy volunteers (P&lt;0.05). The frequencies of scratching of both sides of the nose and sneezing\nwere elevated within 10 min of excitation in asthmatic rats compared with controls. Meanwhile, OVA-sIgE and IL-4\nlevels were significantly higher in asthmatic animals than controls (P&lt;0.05). In the asthma group, narrowed bronchial\nlumen and thickened wall were observed, and bronchial and peripheral vessels showed overt inflammatory cell infiltration.\nEosinophil, neutrophil and mast cell amounts in the BALF of asthmatic mice were significantly higher than\ncontrol values. Furthermore, lung miRNA-126 expression in asthmatic mice was significantly higher than that of\ncontrols. Finally, DNMT1 mRNA and protein levels were significantly lower in asthmatic animals compared with\ncontrols (P &lt; 0.01).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>MiRNA-126 is highly expressed in serum exosomes from allergic asthma patients and lung tissues of\nasthmatic mice, suggesting that it may be involved in the pathogenesis of bronchial asthma.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389200220666191011114452","type":"journal-article","created":{"date-parts":[[2019,10,14]],"date-time":"2019-10-14T05:22:06Z","timestamp":1571030526000},"page":"799-803","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Expression Level of MiRNA-126 in Serum Exosomes of Allergic Asthma Patients and Lung Tissues of Asthmatic Mice"],"prefix":"10.2174","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8713-0606","authenticated-orcid":true,"given":"Meizhen","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6765-8921","authenticated-orcid":true,"given":"Yu-Pei","family":"Li","sequence":"additional","affiliation":[{"name":"China Three Gorges University, Yichang 443002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7759-8886","authenticated-orcid":true,"given":"Xiao-Rui","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-0654","authenticated-orcid":true,"given":"Miao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3312-1370","authenticated-orcid":true,"given":"Shi-Bo","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0726-9219","authenticated-orcid":true,"given":"Yu-Huang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9315-6052","authenticated-orcid":true,"given":"Zu-Hui","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6144-7955","authenticated-orcid":true,"given":"Li-Mei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4496-9377","authenticated-orcid":true,"given":"Xin-Quan","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Longgang E.N.T Hospital, Shenzhen 518172, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/j.aller.2012.04.009","volume":"41","author":"Cimrin A.H.","year":"2013","unstructured":"Cimrin A.H.; Akpinar B.; Tertemiz K.C.; Omeroglu G.; Alpaydin A.O.; Ellidokuz H.; Ozkaya A.B.; Kocturk S.; Phenotype properties and status of corticosteroid resistance among patients with uncontrolled asthma. Allergol Immunopathol (Madr) 2013,41(5),304-309","journal-title":"Allergol Immunopathol (Madr)"},{"key":"ref=2","unstructured":"Global strategy for asthma management and prevention. Global\nInitiative for Asthma (GI-NA)20."},{"key":"ref=3","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1016\/j.jaci.2010.07.019","volume":"126","author":"Bousquet J.","year":"2010","unstructured":"Bousquet J.; Mantzouranis E.; Cruz A.A.; A\u00eft-Khaled N.; Baena-Cagnani C.E.; Bleecker E.R.; Brightling C.E.; Burney P.; Bush A.; Busse W.W.; Casale T.B.; Chan-Yeung M.; Chen R.; Chowdhury B.; Chung K.F.; Dahl R.; Drazen J.M.; Fabbri L.M.; Holgate S.T.; Kauffmann F.; Haahtela T.; Khaltaev N.; Kiley J.P.; Masjedi M.R.; Mohammad Y.; O\u2019Byrne P.; Partridge M.R.; Rabe K.F.; Togias A.; Van Weel C.; Wenzel S.; Zhong N.; Zuberbier T.; Uniform definition of asthma severity, control, and exacerbations: Document presented for the World Health Organization consultation on severe asthma. J Allergy Clin Immunol 2010,126(5),926-938","journal-title":"J Allergy Clin Immunol"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"10466","DOI":"10.1021\/acs.analchem.6b02421","volume":"88","author":"Dold\u00e1n X.","year":"2016","unstructured":"Dold\u00e1n X.; Fag\u00fandez P.; Cayota A.; La\u00edz J.; Tosar J.P.; Electrochemical sandwich immunosensor for determination of exosomes based on surface marker-mediated signal amplification. Anal Chem 2016,88(21),10466-10473","journal-title":"Anal Chem"},{"key":"ref=5","first-page":"629","volume":"5","author":"Bai F.","year":"2015","unstructured":"Bai F.; Pan J.; Research status of exosomes and lung diseases. Int J Respir Sci 2015,5,629-632","journal-title":"Int J Respir Sci"},{"key":"ref=6","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1016\/j.jaci.2016.02.013","volume":"137","author":"Munitz A.","year":"2016","unstructured":"Munitz A.; Karo-Atar D.; Foster P.S.; Asthma diagnosis: MicroRNAs to the rescue. J Allergy Clin Immunol 2016,137(5),1447-1448","journal-title":"J Allergy Clin Immunol"},{"key":"ref=7","doi-asserted-by":"publisher","first-page":"157","DOI":"10.5114\/ada.2016.60606","volume":"33","author":"Ro\u017calski M.","year":"2016","unstructured":"Ro\u017calski M.; Rudnicka L.; Samochocki Z.; MiRNA in atopic dermatitis. Postepy Dermatol Alergol 2016,33(3),157-162","journal-title":"Postepy Dermatol Alergol"},{"key":"ref=8","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1111\/j.1398-9995.2011.02735.x","volume":"67","author":"Kim Y.H.","year":"2012","unstructured":"Kim Y.H.; Yang T.Y.; Park C.S.; Ahn S.H.; Son B.K.; Kim J.H.; Lim D.H.; Jang T.Y.; Anti-IL-33 antibody has a therapeutic effect in a murine model of allergic rhinitis. Allergy 2012,67(2),183-190","journal-title":"Allergy"},{"key":"ref=9","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1038\/nature07204","volume":"454","author":"Galli S.J.","year":"2008","unstructured":"Galli S.J.; Tsai M.; Piliponsky A.M.; The development of allergic inflammation. Nature 2008,454(7203),445-454","journal-title":"Nature"},{"key":"ref=10","doi-asserted-by":"publisher","first-page":"29336","DOI":"10.1074\/jbc.M110.101147","volume":"285","author":"Junaith S.","year":"2010","unstructured":"Junaith S.; Mohamed; Michael A. Lopez; Aladin M. Boriek. Mechanical stretch upregulates microRNA-26a and induces human airway smooth muscle hypertrophy by suppressing glycogen synthase kinase-3beta. JJ Biol Chem 2010,285,29336-29347","journal-title":"JJ Biol Chem"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"894","DOI":"10.1016\/j.jaci.2012.11.039","volume":"131","author":"Lev\u00e4nen B.","year":"2013","unstructured":"Lev\u00e4nen B.; Bhakta N.R.; Paredes P.T.; Barbeau R.; Hiltbrunner S.; ollack, J.L.; Sk\u00f6ld, C.M.; Svartengren, M.; Grunewald, J.; Gabrielsson S.; Eklund A.; Larsson B.M.; Woodruff P.G.; Erle D.J.; Wheelock \u00c5.M. Altered microRNA profliles in bronehoalveolar lavage fluid exosomes in asthmatic patients. J Allergy Clin Immunol 2013,131,894-903","journal-title":"J Allergy Clin Immunol"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"4413","DOI":"10.1007\/s11033-014-3312-5","volume":"41","author":"Verma M.","year":"2014","unstructured":"Verma M.; Chattopadhyay B.D.; Kumar S.; Kumar K.; Verma D.; DNA methyltransferase 1(DNMT1) induced the expression of suppressors of cytokine signaling3 (Socs3) in a mouse model of asthma. Mol Biol Rep 2014,41(7),4413-4424","journal-title":"Mol Biol Rep"}],"container-title":["Current Drug Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/175578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T11:20:20Z","timestamp":1578309620000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/175578\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,12]]},"references-count":12,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,12,12]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1389200220666191011114452","relation":{},"ISSN":["1389-2002"],"issn-type":[{"value":"1389-2002","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12,12]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-24","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}