{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:34:57Z","timestamp":1777541697793,"version":"3.51.4"},"reference-count":23,"publisher":"Bentham Science Publishers Ltd.","issue":"2","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CDM"],"published-print":{"date-parts":[[2021,3,24]]},"abstract":"<jats:sec>\n<jats:title>Introduction:<\/jats:title>\n<jats:p>Infectious diseases have been responsible for an increasing number of deaths worldwide.\nStaphylococcus aureus has been recognized as one of the most notable causative agents of severe infections, while\nefflux pump (EP) expression is one of the main mechanisms associated with S. aureus resistance to antibiotics.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p> This study aimed to investigate the potential of \u03b1-pinene as an efflux pump inhibitor in species of S.\naureus carrying the TetK and MrsA proteins.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>The minimum inhibitory concentrations (MIC) of \u03b1-pinene and other efflux pump inhibitors were assessed\nusing serial dilutions of each compound at an initial concentration above 1024 \u03bcg\/mL. Solutions containing culture\nmedium and bacterial inoculums were prepared in test tubes and subsequently transferred to 96-well microdilution\nplates. The modulation of ethidium bromide (EtBr) and antibiotics (tetracycline and erythromycin) was investigated\nthrough analysis of the modification in their MICs in the presence of a subinhibitory concentration of \u03b1-pinene\n(MIC\/8). Wells containing only culture medium and bacterial inoculums were used as negative control. Carbonyl\ncyanide m-chlorophenylhydrazone (CCCP) was used as a positive control.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>The MIC of ethidium bromide against S. aureus strains RN-4220 and IS-58 was reduced by association with\n\u03b1-pinene. This monoterpene potentiated the effect of tetracycline against the IS-58 strain but failed in modulating the\nantibacterial effect of erythromycin against RN-4220, suggesting a selective inhibitory effect on the TetK EP by \u03b1-\npinene.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p> In conclusion, \u03b1-pinene has promising effects against S.aureus strains, which should be useful in the\ncombat of antibacterial resistance associated with EP expression. Nevertheless, further research is required to fully\ncharacterize its molecular mechanism of action as an EP inhibitor.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389200221999200730212721","type":"journal-article","created":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T10:17:16Z","timestamp":1596622636000},"page":"123-126","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Inhibition of Efflux Pumps by Monoterpene (\u03b1-pinene) and Impact on Staphylococcus aureus Resistance to Tetracycline and Erythromycin"],"prefix":"10.2174","volume":"22","author":[{"given":"Priscilla R.","family":"Freitas","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina J.","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina R.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora F.","family":"Muniz","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saulo R.","family":"Tintino","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Ribeiro-Filho","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 P.","family":"Siqueira J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, UFPB, Joao Pessoa, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.B.","family":"Filho","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Compounds, Federal University of Paraiba, Joao Pessoa, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela R.","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, UFPB, Joao Pessoa, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique D.M.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, URCA, Crato, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"270","DOI":"10.21877\/2448-3877.201800740","volume":"50","author":"Mota F.S.","year":"2018","unstructured":"Mota F.S.; Oliveira H.A.; Souto R.C.F.; Perfil e preval\u00eancia de resist\u00eancia aos antimicrobianos de bact\u00e9rias Gram-negativas isoladas de pacientes de uma unidade de terapia intensiva. 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