{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:49:20Z","timestamp":1740142160999,"version":"3.37.3"},"reference-count":36,"publisher":"Bentham Science Publishers Ltd.","issue":"3","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274010"],"award-info":[{"award-number":["82274010"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18430760400"],"award-info":[{"award-number":["18430760400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Changning Maternity and Infant Health Hospital","award":["2019CNECNUPI02"],"award-info":[{"award-number":["2019CNECNUPI02"]}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CDM"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Carboxylesterase 2 (CES2) is mainly distributed in the human liver and gut, and plays an\nactive role in the metabolic activation of many prodrugs and lipid metabolism. Although CES2 is of great significance,\nthere are still few animal models related to CES2.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objectives:<\/jats:title>\n<jats:p>This research aims to construct Ces2c gene knockout (KO) rats and further study the function of CES2.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>CRISPR\/Cas9 gene editing technology was used to target and cleave the rat Ces2c gene. Compensatory\neffects of major CES subtypes both in the liver and small intestine of KO rats were detected at mRNA levels. Meanwhile,\ndiltiazem and aspirin were used as substrates to test the metabolic capacity of Ces2c in KO rats.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>This Ces2c KO rat model showed normal growth and breeding without off-target effects. The metabolic\nfunction of Ces2c KO rats was verified by the metabolic study of CES2 substrates in vitro. The results showed that\nthe metabolic capacity of diltiazem in KO rats was weakened, while the metabolic ability of aspirin did not change\nsignificantly. In addition, the serum physiological indexes showed that the Ces2c deletion did not affect the liver\nfunction of rats.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>The Ces2c KO rat model was successfully constructed by CRISPR\/Cas9 system. This rat model can not\nonly be used as an important tool to study the drug metabolism mediated by CES2, but also as an important animal\nmodel to study the physiological function of CES2.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389200224666230123140919","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T12:27:56Z","timestamp":1674476876000},"page":"190-199","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design and Construction of Carboxylesterase 2c Gene Knockout Rats by\nCRISPR\/Cas9"],"prefix":"10.2174","volume":"24","author":[{"given":"Jie","family":"Liu","sequence":"first","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuyang","family":"Shang","sequence":"additional","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingyi","family":"Yao","sequence":"additional","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengbo","family":"Huang","sequence":"additional","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanqing","family":"Guo","sequence":"additional","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8079-8638","authenticated-orcid":true,"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Changning Maternity and Infant Health Hospital and School of Life Sciences, Shanghai Key Laboratory of Regulatory Biology, East\nChina Normal University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"91","DOI":"10.2174\/1389200219666180821094502","volume":"20","author":"Di L.","year":"2019","unstructured":"Di L.; The impact of carboxylesterases in drug metabolism and pharmacokinetics. Curr Drug Metab 2019,20(2),91-102","journal-title":"Curr Drug Metab"},{"key":"ref=2","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1002\/jbt.20178","volume":"21","author":"Ross M.K.","year":"2007","unstructured":"Ross M.K.; Crow J.A.; Human carboxylesterases and their role in xenobiotic and endobiotic metabolism. J Biochem Mol Toxicol 2007,21(4),187-196","journal-title":"J Biochem Mol Toxicol"},{"key":"ref=3","doi-asserted-by":"publisher","first-page":"173","DOI":"10.2133\/dmpk.21.173","volume":"21","author":"Imai T.","year":"2006","unstructured":"Imai T.; Human carboxylesterase isozymes: Catalytic properties and rational drug design. Drug Metab Pharmacokinet 2006,21(3),173-185","journal-title":"Drug Metab Pharmacokinet"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1007\/s11095-013-1158-5","volume":"31","author":"Qiu Z.","year":"2014","unstructured":"Qiu Z.; Li N.; Song L.; Lu Y.; Jing J.; Parekha H.S.; Gao W.; Tian F.; Wang X.; Ren S.; Chen X.; Contributions of intestine and plasma to the presystemic bioconversion of vicagrel, an acetate of clopidogrel. Pharm Res 2014,31(1),238-251","journal-title":"Pharm Res"},{"key":"ref=5","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1124\/mol.108.045526","volume":"74","author":"Birkus G.","year":"2008","unstructured":"Birkus G.; Kutty N.; He G.X.; Mulato A.; Lee W.; McDermott M.; Cihlar T.; Activation of 9-[(R)-2-[[(S)-[[(S)-1-(Isopropoxycar-bonyl)ethyl]amino] phenoxyphosphinyl]-methoxy]propyl]adenine (GS-7340) and other tenofovir phosphonoamidate prodrugs by human proteases. Mol Pharmacol 2008,74(1),92-100","journal-title":"Mol Pharmacol"},{"key":"ref=6","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1002\/phar.1194","volume":"33","author":"Casey Laizure S.","year":"2013","unstructured":"Casey Laizure S.; Herring V.; Hu Z.; Witbrodt K.; Parker R.B.; The role of human carboxylesterases in drug metabolism: Have we overlooked their importance? Pharmacotherapy 2013,33(2),210-222","journal-title":"Pharmacotherapy"},{"key":"ref=7","first-page":"2605","volume":"8","author":"Xu G.","year":"2002","unstructured":"Xu G.; Zhang W.; Ma M.K.; McLeod H.L.; Human carboxylesterase 2 is commonly expressed in tumor tissue and is correlated with activation of irinotecan. Clin Cancer Res 2002,8(8),2605-2611","journal-title":"Clin Cancer Res"},{"key":"ref=8","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.cbpc.2017.01.008","volume":"194","author":"Takumi S.","year":"2017","unstructured":"Takumi S.; Shimono T.; Ikema S.; Hotta Y.; Chigwechokha P.K.; Shiozaki K.; Sugiyama Y.; Hashimoto M.; Furukawa T.; Komatsu M.; Overexpression of carboxylesterase contributes to the attenuation of cyanotoxin microcystin-LR toxicity. Comp Biochem Physiol C Toxicol Pharmacol 2017,194,22-27","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"ref=9","doi-asserted-by":"publisher","first-page":"1482","DOI":"10.1124\/dmd.105.005587","volume":"33","author":"Kubo T.","year":"2005","unstructured":"Kubo T.; Kim S.R.; Sai K.; Saito Y.; Nakajima T.; Matsumoto K.; Saito H.; Shirao K.; Yamamoto N.; Minami H.; Ohtsu A.; Yoshida T.; Saijo N.; Ohno Y.; Ozawa S.; Sawada J.; Functional characterization of three naturally occurring single nucleotide polymorphisms in the CES2 gene encoding carboxylesterase 2 (HCE-2). Drug Metab Dispos 2005,33(10),1482-1487","journal-title":"Drug Metab Dispos"},{"key":"ref=10","doi-asserted-by":"publisher","first-page":"327","DOI":"10.2133\/dmpk.18.327","volume":"18","author":"Kim S.R.","year":"2003","unstructured":"Kim S.R.; Nakamura T.; Saito Y.; Sai K.; Nakajima T.; Saito H.; Shirao K.; Minami H.; Ohtsu A.; Yoshida T.; Saijo N.; Ozawa S.; Sawada J.; Twelve novel single nucleotide polymorphisms in the CES2 gene encoding human carboxylesterase 2 (hCE-2). Drug Metab Pharmacokinet 2003,18(5),327-332","journal-title":"Drug Metab Pharmacokinet"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.phrs.2017.08.005","volume":"128","author":"Lam S.W.","year":"2018","unstructured":"Lam S.W.; van der Noort V.; van der Straaten T.; Honkoop A.H.; Peters G.J.; Guchelaar H.J.; Boven E.; Single-nucleotide polymorphisms in the genes of CES2, CDA and enzymatic activity of CDA for prediction of the efficacy of capecitabine-containing chemotherapy in patients with metastatic breast cancer. Pharmacol Res 2018,128,122-129","journal-title":"Pharmacol Res"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1093\/jn\/130.2.340S","volume":"130","author":"Harrison E. H.","year":"2000","unstructured":"Harrison E. H.; Lipases and carboxylesterases: Possible roles in the hepatic utilization of vitamin A J Nutr 2000,130((2S Suppl)),340-344","journal-title":"A J Nutr"},{"key":"ref=13","doi-asserted-by":"publisher","first-page":"4387","DOI":"10.1046\/j.1432-1033.2002.03121.x","volume":"269","author":"Sanghani S.P.","year":"2002","unstructured":"Sanghani S.P.; Davis W.I.; Dumaual N.G.; Mahrenholz A.; Bosron W.F.; Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate. Eur J Biochem 2002,269(18),4387-4398","journal-title":"Eur J Biochem"},{"key":"ref=14","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s00335-010-9284-4","volume":"21","author":"Holmes R.S.","year":"2010","unstructured":"Holmes R.S.; Wright M.W.; Laulederkind S.J.F.; Cox L.A.; Hosokawa M.; Imai T.; Ishibashi S.; Lehner R.; Miyazaki M.; Perkins E.J.; Potter P.M.; Redinbo M.R.; Robert J.; Satoh T.; Yamashita T.; Yan B.; Yokoi T.; Zechner R.; Maltais L.J.; Recommended nomenclature for five mammalian carboxylesterase gene families: Human, mouse, and rat genes and proteins. Mamm Genome 2010,21(9-10),427-441","journal-title":"Mamm Genome"},{"key":"ref=15","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.dmpk.2014.12.001","volume":"30","author":"Oda S.","year":"2015","unstructured":"Oda S.; Fukami T.; Yokoi T.; Nakajima M.; A comprehensive review of UDP-glucuronosyltransferase and esterases for drug development. Drug Metab Pharmacokinet 2015,30(1),30-51","journal-title":"Drug Metab Pharmacokinet"},{"key":"ref=16","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.bcp.2016.03.001","volume":"105","author":"Wang X.","year":"2016","unstructured":"Wang X.; Tang Y.; Lu J.; Shao Y.; Qin X.; Li Y.; Wang L.; Li D.; Liu M.; Characterization of novel cytochrome P450 2E1 knockout rat model generated by CRISPR\/Cas9. Biochem Pharmacol 2016,105,80-90","journal-title":"Biochem Pharmacol"},{"key":"ref=17","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1124\/dmd.120.000114","volume":"48","author":"Lu J.","year":"2020","unstructured":"Lu J.; Chen A.; Ma X.; Shang X.; Zhang Y.; Guo Y.; Liu M.; Wang X.; Generation and characterization of cytochrome P450 2J3\/10 CRISPR\/Cas9 knockout rat model. Drug Metab Dispos 2020,48(11),1129-1136","journal-title":"Drug Metab Dispos"},{"key":"ref=18","doi-asserted-by":"publisher","first-page":"2973","DOI":"10.1016\/j.apsb.2021.01.007","volume":"11","author":"Lu J.","year":"2021","unstructured":"Lu J.; Liu J.; Guo Y.; Zhang Y.; Xu Y.; Wang X.; CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics. Acta Pharm Sin B 2021,11(10),2973-2982","journal-title":"Acta Pharm Sin B"},{"key":"ref=19","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1124\/molpharm.121.000357","volume":"100","author":"Liu J.","year":"2021","unstructured":"Liu J.; Shang X.; Huang S.; Xu Y.; Lu J.; Zhang Y.; Liu Z.; Wang X.; Construction and characterization of CRISPR\/Cas9 knockout rat model of carboxylesterase 2a gene. Mol Pharmacol 2021,100(5),480-490","journal-title":"Mol Pharmacol"},{"key":"ref=20","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1002\/hep4.1292","volume":"3","author":"Maresch L.K.","year":"2019","unstructured":"Maresch L.K.; Benedikt P.; Feiler U.; Eder S.; Zierler K.A.; Taschler U.; Kolleritsch S.; Eichmann T.O.; Schoiswohl G.; Leopold C.; Wieser B.I.; Lackner C.; R\u00fclicke T.; van Klinken J.; Kratky D.; Moustafa T.; Hoefler G.; Haemmerle G.; Intestine-specific overexpression of carboxylesterase 2c protects mice from diet-induced liver steatosis and obesity. Hepatol Commun 2019,3(2),227-245","journal-title":"Hepatol Commun"},{"key":"ref=21","first-page":"4983","volume":"9","author":"Sanghani S.P.","year":"2003","unstructured":"Sanghani S.P.; Quinney S.K.; Fredenburg T.B.; Sun Z.; Davis W.I.; Murry D.J.; Cummings O.W.; Seitz D.E.; Bosron W.F.; Carboxylesterases expressed in human colon tumor tissue and their role in CPT-11 hydrolysis. Clin Cancer Res 2003,9(13),4983-4991","journal-title":"Clin Cancer Res"},{"key":"ref=22","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1007\/s13238-017-0437-z","volume":"9","author":"Lian J.","year":"2018","unstructured":"Lian J.; Nelson R.; Lehner R.; Carboxylesterases in lipid metabolism: From mouse to human. Protein Cell 2018,9(2),178-195","journal-title":"Protein Cell"},{"key":"ref=23","doi-asserted-by":"publisher","first-page":"1064","DOI":"10.3109\/00498254.2013.802059","volume":"43","author":"Ozaki H.","year":"2013","unstructured":"Ozaki H.; Sugihara K.; Watanabe Y.; Fujino C.; Uramaru N.; Sone T.; Ohta S.; Kitamura S.; Comparative study of the hydrolytic metabolism of methyl-, ethyl-, propyl-, butyl-, heptyl- and dodecylparaben by microsomes of various rat and human tissues. Xenobiotica 2013,43(12),1064-1072","journal-title":"Xenobiotica"},{"key":"ref=24","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1161\/CIRCRESAHA.112.267468","volume":"111","author":"Lian J.","year":"2012","unstructured":"Lian J.; Quiroga A.D.; Li L.; Lehner R.; Ces3\/TGH deficiency improves dyslipidemia and reduces atherosclerosis in Ldlr(-\/-) mice. Circ Res 2012,111(8),982-990","journal-title":"Circ Res"},{"key":"ref=25","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.cbd.2009.03.002","volume":"4","author":"Holmes R.S.","year":"2009","unstructured":"Holmes R.S.; Cox L.A.; VandeBerg J.L.; A new class of mammalian carboxylesterase CES6. Comp. Biochem. Physiol. Part D Genom. Proteom 2009,4(3),209-217","journal-title":"Proteom"},{"key":"ref=26","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1016\/j.apsb.2019.10.001","volume":"9","author":"Li Y.","year":"2019","unstructured":"Li Y.; Meng Q.; Yang M.; Liu D.; Hou X.; Tang L.; Wang X.; Lyu Y.; Chen X.; Liu K.; Yu A.M.; Zuo Z.; Bi H.; Current trends in drug metabolism and pharmacokinetics. Acta Pharm Sin B 2019,9(6),1113-1144","journal-title":"Acta Pharm Sin B"},{"key":"ref=27","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1038\/90761","volume":"19","author":"Hodgson J.","year":"2001","unstructured":"Hodgson J.; ADMET-turning chemicals into drugs. Nat Biotechnol 2001,19(8),722-726","journal-title":"Nat Biotechnol"},{"key":"ref=28","doi-asserted-by":"publisher","first-page":"793","DOI":"10.2174\/138920010794328904","volume":"11","author":"Imai T.","year":"2010","unstructured":"Imai T.; Ohura K.; The role of intestinal carboxylesterase in the oral absorption of prodrugs. Curr Drug Metab 2010,11(9),793-805","journal-title":"Curr Drug Metab"},{"key":"ref=29","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1124\/dmd.106.013862","volume":"35","author":"Masaki K.","year":"2007","unstructured":"Masaki K.; Hashimoto M.; Imai T.; Intestinal first-pass metabolism via carboxylesterase in rat jejunum and ileum. Drug Metab Dispos 2007,35(7),1089-1095","journal-title":"Drug Metab Dispos"},{"key":"ref=30","doi-asserted-by":"publisher","first-page":"42922","DOI":"10.1038\/srep42922","volume":"7","author":"Lu J.","year":"2017","unstructured":"Lu J.; Shao Y.; Qin X.; Liu D.; Chen A.; Li D.; Liu M.; Wang X.; CRISPR knockout rat cytochrome P450 3A1\/2 model for advancing drug metabolism and pharmacokinetics research. Sci Rep 2017,7(1),42922","journal-title":"Sci Rep"},{"key":"ref=31","doi-asserted-by":"publisher","first-page":"2188","DOI":"10.1002\/hep.25961","volume":"56","author":"Quiroga A.D.","year":"2012","unstructured":"Quiroga A.D.; Li L.; Tr\u00f6tzm\u00fcller M.; Nelson R.; Proctor S.D.; K\u00f6feler H.; Lehner R.; Deficiency of carboxylesterase 1\/esterase-x results in obesity, hepatic steatosis, and hyperlipidemia. Hepatology 2012,56(6),2188-2198","journal-title":"Hepatology"},{"key":"ref=32","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1124\/dmd.121.000403","volume":"49","author":"Sun D.","year":"2021","unstructured":"Sun D.; Lu J.; Zhang Y.; Liu J.; Liu Z.; Yao B.; Guo Y.; Wang X.; Characterization of a novel CYP1A2 knockout rat model constructed by CRISPR\/Cas9. Drug Metab Dispos 2021,49(8),638-647","journal-title":"Drug Metab Dispos"},{"key":"ref=33","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1124\/dmd.118.084277","volume":"47","author":"Liang C.","year":"2019","unstructured":"Liang C.; Zhao J.; Lu J.; Zhang Y.; Ma X.; Shang X.; Li Y.; Ma X.; Liu M.; Wang X.; Development and characterization of MDR1 (Mdr1a\/b) CRISPR\/Cas9 knockout rat model. Drug Metab Dispos 2019,47(2),71-79","journal-title":"Drug Metab Dispos"},{"key":"ref=34","doi-asserted-by":"publisher","first-page":"1860","DOI":"10.1002\/hep.28472","volume":"63","author":"Li Y.","year":"2016","unstructured":"Li Y.; Zalzala M.; Jadhav K.; Xu Y.; Kasumov T.; Yin L.; Zhang Y.; Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice. Hepatology 2016,63(6),1860-1874","journal-title":"Hepatology"},{"key":"ref=35","doi-asserted-by":"publisher","first-page":"468","DOI":"10.2133\/dmpk.DMPK-12-RG-143","volume":"28","author":"Yanjiao X.","year":"2013","unstructured":"Yanjiao X.; Chengliang Z.; Xiping L.; Tao W.; Xiuhua R.; Dong L.; Evaluation of the inhibitory effects of antihypertensive drugs on human carboxylesterase in vitro. Drug Metab Pharmacokinet 2013,28(6),468-474","journal-title":"Drug Metab Pharmacokinet"},{"key":"ref=36","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1124\/dmd.113.054551","volume":"42","author":"Ohura K.","year":"2014","unstructured":"Ohura K.; Tasaka K.; Hashimoto M.; Imai T.; Distinct patterns of aging effects on the expression and activity of carboxylesterases in rat liver and intestine. Drug Metab Dispos 2014,42(2),264-273","journal-title":"Drug Metab Dispos"}],"container-title":["Current Drug Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/1389200224666230123140919","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/213024\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/1389200224666230123140919","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T12:19:55Z","timestamp":1694175595000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/213024\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1389200224666230123140919","relation":{},"ISSN":["1389-2002"],"issn-type":[{"type":"print","value":"1389-2002"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2022-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-05","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}