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However, drug abuse and BZDs' illegal addition may affect normal life and even lead to grave social harm. As BZDs may be metabolized and eliminated quickly, it is of great theoretical and practical significance to clarify their metabolic profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective:<\/jats:title><jats:p>In this paper, LC-Q-TOF\/MS-based fragmentation behavior has been investigated for nine benzodiazepine drugs available and widely used in clinical treatment (diazepam, nitrazepam, clonazepam, oxazepam, lorazepam, alprazolam, estazolam, triazolam, and midazolam), and their metabolic profile has been studied by using in vitro human liver microsomal incubation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>A regular human liver microsomal system was used to investigate the potential biotransformation of the nine benzodiazepines in vitro, and an LC-Q\/TOF-MS was used to perform fragmentation behavior studies and metabolite identification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>As a result, characteristic fragmentation pathway and diagnostic fragment ions of the nine BZDs were analyzed, and 19 metabolites of the 9 benzodiazepines were found and identified, with glucuronidation and hydroxylation considered as their most important metabolic pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>These experimental data add to our knowledge of the nine benzodiazepine drugs and their metabolism study, which could provide useful information and evidence of their in vivo metabolic profile prediction and help promote their monitoring in both clinical use and social\/illegal abuse.<\/jats:p><\/jats:sec>","DOI":"10.2174\/1389200224666230419090733","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T06:34:05Z","timestamp":1683095645000},"page":"223-237","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["LC-Q-TOF\/MS-based Fragmentation Behavior Study and In vitro Metabolites Identification of Nine Benzodiazepines"],"prefix":"10.2174","volume":"24","author":[{"given":"Shuhan","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoting","family":"Song","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengkai","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyi","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqi","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Forensic Science, Jiangsu Police Institute, Nanjing 210031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Forensic Science, Jiangsu Police Institute, Nanjing 210031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3926-5124","authenticated-orcid":true,"given":"Liang","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"},{"name":"State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","author":"Sadock B.J.","year":"2005","unstructured":"Sadock B.J.; Sadock V.A.; Kaplan and Sadock\u2019s Comprehensive Textbook of Psychiatry 2005","journal-title":"Kaplan and Sadock\u2019s Comprehensive Textbook of Psychiatry"},{"key":"ref=2","first-page":"70","volume":"23","author":"Si T.M.","year":"2017","unstructured":"Si T.M.; Liu T.Q.; Zhang C.H.; Jia F.J.; Guo W.J.; Wang G.H.; Wang C.Y.; Niu Y.J.; Liu T.B.; Xiang X.J.; Zhao M.; The pharmacology of benzodiazepines. 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