{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T21:01:17Z","timestamp":1772658077951,"version":"3.50.1"},"reference-count":24,"publisher":"Bentham Science Publishers Ltd.","issue":"6","license":[{"start":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T00:00:00Z","timestamp":1562630400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPB"],"published-print":{"date-parts":[[2019,7,9]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>The study aimed to investigate the effects of the active ingredient, nimodipine,\non chondrocyte proliferation and extracellular matrix (ECM) structures in cartilage tissue cells.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Chondrocyte cultures were prepared from tissues resected via surgical operations. Nimodipine\nwas then applied to these cultures and molecular analysis was performed. The data obtained were\nstatistically calculated.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Both, the results of the (3-(4,5 dimethylthiazol2-yl)-2,5-diphenyltetrazolium (MTT) assay and\nthe fluorescence microscope analysis [a membrane permeability test carried out with acridine orange\/\npropidium iodide staining (AO\/PI)] confirmed that the active ingredient, nimodipine, negatively\naffects the cell cultures.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Nimodipine was reported to suppress cellular proliferation; chondroadherin (CHAD) and\nhypoxia-inducible factor-1 alpha (HIF-1\u03b1) expression thus decreased by 2.4 and 1.7 times, respectively,\nat 24 hrs when compared to the control group (p &lt; 0.05). Furthermore, type II collagen\n(COL2A1) expression was not detected (p&lt;0.05). The risk that a drug prescribed by a clinician in an\ninnocuous manner to treat a patient by relieving the symptoms of a disease may affect the proliferation,\ndifferentiation, and viability of other cells and\/or tissues at the molecular level, beyond its known side\neffects or adverse events, should not be forgotten.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389201020666190506124548","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T03:36:13Z","timestamp":1557200173000},"page":"517-524","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Does Nimodipine, a Selective Calcium Channel Blocker, Impair Chondrocyte Proliferation or Damage Extracellular Matrix Structures?"],"prefix":"10.2174","volume":"20","author":[{"given":"Necati","family":"Kaplan","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Corlu Reyap Hospital, Istanbul Rumeli University, 59680, Tekirdag, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibrahim","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Istanbul Medipol University School of Medicine, 34810, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Numan","family":"Karaarslan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Namik Kemal University School of Medicine, 59100, Tekirdag, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasin E.","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Orthopedic and Traumatology, Abant Izzet Basal University School of Medicine, 14000, Bolu, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duygu Y.","family":"Sirin","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, 59100, Tekirdag, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanefi","family":"Ozbek","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Istanbul Medipol University School of Medicine, 34810, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","first-page":"361","volume":"51","author":"Pillai S.V.","year":"2003","unstructured":"Pillai SV, Kolluri VR, Mohanty A, Chandramouli BA.  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[PMID: 16921238].","journal-title":"Dev Neurosci"}],"container-title":["Current Pharmaceutical Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/171999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T15:03:36Z","timestamp":1566486216000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/171999\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,9]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,7,9]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1389201020666190506124548","relation":{},"ISSN":["1389-2010"],"issn-type":[{"value":"1389-2010","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7,9]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-01","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}