{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T07:11:53Z","timestamp":1761808313503,"version":"3.37.3"},"reference-count":87,"publisher":"Bentham Science Publishers Ltd.","issue":"9","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPB"],"published-print":{"date-parts":[[2020,6,9]]},"abstract":"<jats:sec>\n<jats:title\/>\n<jats:p>Current cardiac safety assessment platforms (in vitro hERG-centric, APD, and\/or in vivo animal\nQT assays) are not fully predictive of drug-induced Torsades de Pointes (TdP) and do not address\nother mechanism-based arrhythmia, including ventricular tachycardia or ventricular fibrillation, or cardiac\nsafety liabilities such as contractile and structural cardiotoxicity which are another growing safety\nconcerns. We organized the Consortium for Safety Assessment using Human iPS cells (CSAHi;\nhttp:\/\/csahi.org\/en\/) in 2013, based on the Japan Pharmaceutical Manufacturers Association (JPMA), to\nverify the application of human iPS\/ES cell-derived cardiomyocytes for drug safety evaluation. The\nCSAHi HEART team focused on comprehensive screening strategies to predict a diverse range of cardiotoxicities\nusing recently introduced platforms such as the Multi-Electrode Array (MEA), cellular\nimpedance, Motion Field Imaging (MFI), and optical imaging of Ca transient to identify strengths and\nweaknesses of each platform. Our study showed that hiPS-CMs used in these platforms could detect\npharmacological responses that were more relevant to humans compared to existing hERG, APD, or\nLangendorff (MAPD\/contraction) assays. Further, MEA and other methods such as impedance, MFI,\nand Ca transient assays provided paradigm changes of platforms for predicting drug-induced QT risk\nand\/or arrhythmia or contractile dysfunctions. In contrast, since discordances such as overestimation\n(false positive) of arrhythmogenicity, oversight, or opposite conclusions in positive inotropic and negative\nchronotropic activities to some compounds were also confirmed, possibly due to their functional\nimmaturity of hiPS-CMs, hiPS-CMs should be used in these platforms for cardiac safety assessment\nbased upon their advantages and disadvantages.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389201020666191024172425","type":"journal-article","created":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T14:09:45Z","timestamp":1572271785000},"page":"829-841","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comprehensive Cardiac Safety Assessment using hiPS-cardiomyocytes (Consortium for Safety Assessment using Human iPS Cells: CSAHi)"],"prefix":"10.2174","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5006-895X","authenticated-orcid":true,"given":"Kiyoshi","family":"Takasuna","sequence":"first","affiliation":[{"name":"Consortium for Safety Assessment using Human iPS Cells (CSAHi), Heart Team, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki","family":"Kazusa","sequence":"additional","affiliation":[{"name":"Consortium for Safety Assessment using Human iPS cells (CSAHi), Heart team, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Hayakawa","sequence":"additional","affiliation":[{"name":"Consortium for Safety Assessment using Human iPS cells (CSAHi), Heart team, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1038\/nrd4083","volume":"12(8)","author":"Chi,K.R","year":"2013","unstructured":"Chi.; K.R. 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