{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:15:24Z","timestamp":1791267324583,"version":"4.1.0"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"14","funder":[{"DOI":"10.13039\/100005454","name":"Chinese Medicine Project of Jiangxi Health and Family Planning Commission in 2014","doi-asserted-by":"publisher","award":["2014A159"],"award-info":[{"award-number":["2014A159"]}],"id":[{"id":"10.13039\/100005454","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPB"],"published-print":{"date-parts":[[2021,10,6]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p> Asthenozoospermia, also known as lack of sperm motility, accounts for about\n27.8% of male infertility as a separate factor, and is often associated with abnormal quantity and morphology\nof spermatozoa. Therefore, oligozoospermia has become one of the most important factors affecting\nmale infertility.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Ursolic Acid (UA), also known as wusu acid, is the main active component isolated from\nPrunella vulgaris L. and has a variety of pharmacological effects. However, the protective effect of\nUA on asthenozoospermia disease has not been reported. In the current study, the purpose of this study\nwas to investigate the regulatory effect of UA in rats with LPS-induced asthenozoospermia disease. SD\nrats were treated with 5 mg\/kg LPS, respectively.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>After different concentrations of UA were infused into the stomach of SD rats, microscopy,\nflow cytometry, Enzyme-Linked Immunosorbent Assay (ELISA), qRT-PCR and western blot were\nused to detect sperm motility, apoptosis, the levels of TNF-\u03b1, IL-1\u03b2 and IL-6, and Bcl-2\/Bax apoptosis\npathway related proteins in rat serum and epididymis tissues.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Discussion:<\/jats:title>\n<jats:p>Compared with the normal group, the sperm motility and Bcl-2 level in LPS group decreased\nsignificantly, while the expression of inflammatory factors and Bax proteins increased significantly\n(P&lt;0.05). Compared with LPS group, UA intervention group has the opposite result and dose\ndependence.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>This study shows that UA can protect LPS-induced asthenozoospermia of rats by increasing\nsperm density and motility, regulating Bcl-2\/Bax apoptosis pathway and reducing inflammatory\napoptosis response. This experiment provides ideas for improving the clinical treatment of infertile patients\nwith oligoasthenospermia.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389201021666201027155413","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T06:19:32Z","timestamp":1603865972000},"page":"1953-1959","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Protective Effect of Ursolic Acid in Prunella vulgaris L. on LPS-Induced Asthenozoospermia via Bcl-2\/Bax Apoptosis Signaling Pathway"],"prefix":"10.2174","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5897-9045","authenticated-orcid":true,"given":"Xiaoyong","family":"Sun","sequence":"first","affiliation":[{"name":"Surgery of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4838-4093","authenticated-orcid":true,"given":"Xiaobo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5473-5318","authenticated-orcid":true,"given":"Shengjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chinese Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5752-9114","authenticated-orcid":true,"given":"Jinfeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anorectal, Ganzhou Hospital of Traditional Chinese Medicine, Ganzhou, Jiangxi 341000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7628-7605","authenticated-orcid":true,"given":"Bin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Chinese Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1525-1107","authenticated-orcid":true,"given":"Guozheng","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology, Yunnan Provincial Hospital of Traditional Chinese Medicine, 120 Guanghua Street, Kunming, Yunnan 650000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","container-title":["Current Pharmaceutical Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/187262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/187262\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/187262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T06:13:15Z","timestamp":1633932795000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/187262\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,6]]},"references-count":0,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2021,10,6]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1389201021666201027155413","relation":{},"ISSN":["1389-2010"],"issn-type":[{"value":"1389-2010","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10,6]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2020-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-11","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}