{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T21:09:26Z","timestamp":1748380166212,"version":"3.37.3"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"14","funder":[{"DOI":"10.13039\/501100017584","name":"Beijing Youth Talent Project","doi-asserted-by":"publisher","award":["CIT&TCD201904052"],"award-info":[{"award-number":["CIT&TCD201904052"]}],"id":[{"id":"10.13039\/501100017584","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012492","name":"Beijing Outstanding Talent Training Program","doi-asserted-by":"publisher","award":["2017000026833ZK18"],"award-info":[{"award-number":["2017000026833ZK18"]}],"id":[{"id":"10.13039\/100012492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPB"],"published-print":{"date-parts":[[2021,10,6]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>The ability of pathogenic bacteria to survive Antimicrobial Peptides (AMPs)\nin various host niches may contribute to their virulence. Polymyxin B is a cationic AMP, and polymyxin\ndrugs are considered to be the \"last line of defense\" in the clinical treatment of bacterial infections.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>The objectives of this study were to comprehensively study the response of Brucella\nmelitensis strain NI to polymyxin B treatment and to identify the target genes in Brucella induced by\npolymyxin B stimulation.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Following treatment with polymyxin B, differentially expressed genes in Brucella were detected\nusing RNA-seq and validated using qRT-PCR.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p> In total, 874 differentially expressed genes were identified, including 560 up-regulated and\n314 down-regulated genes. Functional annotation and KEGG pathway analysis revealed that many of\nthese genes are involved in metabolism, two-component systems, transcriptional regulation, transport\/\nmembrane proteins, and virulence factors. Expression of genes involved in T4SS and flagellar biosynthesis\nand assembly, which are important virulence factors in Brucella, were up-regulated by polymyxin\nB treatment.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Discussion:<\/jats:title>\n<jats:p>Additionally, genes encoding the ABC transporters YejABEF and the cold-shock protein\nCspA were also up-regulated. These genes confer resistance to AMPs and contribute to the virulence\nof Brucella. The NI\u0394sufC, NI\u0394sufD, NI\u0394ompW, NI\u0394exbB, NI\u0394tetR, and NI\u0394cspA mutants were also\nmore sensitive than B. melitensis NI to polymyxin B.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>The results of this study provide important insights into the comprehensive response of\nBrucella in response to polymyxin B stimulation.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389201021666201218125148","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T05:56:05Z","timestamp":1608530165000},"page":"1891-1899","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome-Wide Analysis of the Response of Brucella melitensis NI to Polymyxin B"],"prefix":"10.2174","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4002-7892","authenticated-orcid":true,"given":"Zhen","family":"Wang","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0977-6177","authenticated-orcid":true,"given":"Jiawei","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9042-0900","authenticated-orcid":true,"given":"Jie","family":"Cheng","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5685-0766","authenticated-orcid":true,"given":"Qingmin","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Current Pharmaceutical Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/189251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/189251\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/189251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T11:13:21Z","timestamp":1633950801000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/189251\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,6]]},"references-count":0,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2021,10,6]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1389201021666201218125148","relation":{},"ISSN":["1389-2010"],"issn-type":[{"type":"print","value":"1389-2010"}],"subject":[],"published":{"date-parts":[[2021,10,6]]},"assertion":[{"value":"Peer 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