{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T20:15:25Z","timestamp":1767212125418,"version":"3.37.3"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"12","funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["2017ZC0180"],"award-info":[{"award-number":["2017ZC0180"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CPB"],"published-print":{"date-parts":[[2021,9,2]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>The pathogenesis of Severe Acute Pancreatitis (SAP) is mediated substantially\nby dysfunctions in the intestinal barrier. Euphorbia kansui (EK) is a medicinal plant used\nwidely in traditional Chinese medicine to treat inflammation; however, its efficacy and mechanism\nof action in SAP treatment are not yet well understood.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>To investigate the role of EK in intestinal barrier tissue repair and in the pathogenesis\nand development of SAP.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>The rat SAP model was established by a retrograde injection of sodium taurocholate into\nthe pancreatic bile duct. The SAP model group and the SAP + EK treatment groups were divided\ninto 6 subgroups according to timing: 2, 6, 12, 24, 48, or 72h after inducing SAP. The progression\nof the SAP rats and of the rats receiving the EK treatment was evaluated using the ascites volume,\nserum amylase and plasma endotoxin levels, and histological grading of intestinal mucosal damage.\nIn addition, serum inflammatory factor contents were measured using Enzyme-Linked Immunosorbent\nAssay (ELISA) tests and apoptotic cells in damaged ileum tissue were detected using\nTUNEL staining. Apoptosis markers and other signaling proteins in intestinal mucosal cells were\ndetected by immunohistochemical assays and then validated by combining these data with quantitative\npolymerase chain reactions and western blotting.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Compared with the results of the SAP model rats, the results of the rats that received EK\ntreatment demonstrated that EK could effectively reduce the ascites volume and serum amylase and\nplasma endotoxin levels. EK treatment also greatly reduced the abnormal intestinal morphological\nalterations in the rat SAP model and significantly downregulated the serum contents of Interleukin\n(IL)-1\u03b2, IL-6, and tumor necrosis factor-\u03b1. EK treatment inhibited the elevation of capapse-3, inhibited\nthe decrease of the Bcl-2 protein, and decreased the number of apoptotic cells in rat ileum tissue.\nFinally, EK treatment abrogated the increase of HMGB1 and the suppression of MFG-E8 protein\nexpression in the SAP + EK rat ileum tissue.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>EK suppresses SAP pathogenesis by restoring the intestinal barrier function and modulating\nthe HMGB1\/MFG-E8 signaling axis.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1389201021666201218130440","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T05:56:05Z","timestamp":1608530165000},"page":"1672-1682","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Restoration of Intestinal Mucosa in Euphorbia kansui-treated Severe Acute Pancreatitis Rats based on HMGB1\/MFG-E8 Expression"],"prefix":"10.2174","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2179-5079","authenticated-orcid":true,"given":"Chengjiang","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Abdominal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3490-5935","authenticated-orcid":true,"given":"Kairui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Abdominal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8774-9801","authenticated-orcid":true,"given":"Xuguang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Abdominal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weirun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Abdominal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0120-8175","authenticated-orcid":true,"given":"Sheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Abdominal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0303-4102","authenticated-orcid":true,"given":"Youxing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Abdominal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Current Pharmaceutical Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/189253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/189253\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/189253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T18:08:09Z","timestamp":1630951689000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/189253\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,2]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,9,2]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1389201021666201218130440","relation":{},"ISSN":["1389-2010"],"issn-type":[{"type":"print","value":"1389-2010"}],"subject":[],"published":{"date-parts":[[2021,9,2]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2020-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-30","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}