{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:44:09Z","timestamp":1787024649470,"version":"build-2736575974"},"reference-count":47,"publisher":"Bentham Science Publishers Ltd.","issue":"5","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CGT"],"published-print":{"date-parts":[[2019,12,27]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>MicroRNA modulation therapy has shown great promise to treat hepatocellular\ncarcinoma (HCC), however Efficient tissue-specific and safe delivery remains a major challenge.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>We sought to develop an inorganic-organic hybrid vehicle for the systemic delivery of the\ntumor suppressor miR-34a, and to investigate the efficiency of the delivered miR-34a in the treatment\nof HCC in vitro and in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In the present study, pEGP-miR cloning and expression vector, expressing miR-34a, was\nelectrostatically bound to polyethyleneimine (PEI), and then loaded onto ZSM-5 zeolite nanoparticles\n(ZNP). Qualitative and quantitative assessment of the transfection efficiency of miR-34a construct in\nHepG2 cells was applied by GFP screening and qRT-PCR, respectively. The expression of miR-34a\ntarget genes was investigated by qRT-PCR in vitro and in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>ZNP\/PEI\/miR-34a nano-formulation could efficiently deliver into HepG2 cells with low cytotoxicity,\nindicating good biocompatibility of generated nanozeolite. Furthermore, five injected doses\nof ZNP\/PEI\/miR-34a nano-formulation in HCC induced male Balb-c mice, significantly inhibited tumor\ngrowth, and demonstrated improved cell structure, in addition to a significant decrease in alphafetoprotein\nlevel and liver enzymes activities, as compared to the positive control group. Moreover,\ninjected ZNP\/PEI\/miR-34a nano-formulation led to a noticeable decrease in the CD44 and c-Myc levels.\nResults also showed that ZNP\/PEI\/miR-34a nano-formulation inhibited several target oncogenes\nincluding AEG-1, and SOX-9, in vitro and in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Our results suggested that miR-34a is a powerful candidate in HCC treatment and that\nAEG-1 and SOX-9 are novel oncotargets of miR-34a in HCC. Results also demonstrated that our\nnano-formulation may serve as a candidate approach for miR-34a restoration for HCC therapy, and\ngenerally for safe gene delivery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2174\/1566523219666191108103739","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T07:37:08Z","timestamp":1573198628000},"page":"342-354","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Effect of Tumor Suppressor MiR-34a Loaded on ZSM-5 Nanozeolite in Hepatocellular Carcinoma: In Vitro and In Vivo Approach"],"prefix":"10.2174","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3501-4464","authenticated-orcid":true,"given":"Zeinab","family":"Salah","sequence":"first","affiliation":[{"name":"Biomedical Informatics and Chemo-Informatics Group Leader, Centre of Excellence for Medical Research, Informatics and System Dept, National Research Centre (NRC), Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2105-3817","authenticated-orcid":true,"given":"Eman M.","family":"Abd El Azeem","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1032-8312","authenticated-orcid":true,"given":"Hanan F.","family":"Youssef","sequence":"additional","affiliation":[{"name":"Refractories, Ceramics and Building Materials Department, National Research Centre (NRC), Dokki, Cairo 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4423-5616","authenticated-orcid":true,"given":"Amira M.","family":"Gamal-Eldeen","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Al Mutamarat Rd, Al Mathnah, At Taif 26521, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6121-450X","authenticated-orcid":true,"given":"Abdel R.","family":"Farrag","sequence":"additional","affiliation":[{"name":"Pathology Department Medical Division Research, National Research Centre, Cairo, 12622, Dokki, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4953-6557","authenticated-orcid":true,"given":"Emad","family":"El-Meliegy","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, National Research Centre, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8942-8246","authenticated-orcid":true,"given":"Bangly","family":"Soliman","sequence":"additional","affiliation":[{"name":"Biomedical Informatics and Chemo-Informatics Group Leader, Centre of Excellence for Medical Research, Informatics and System Dept, National Research Centre (NRC), Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3896-6911","authenticated-orcid":true,"given":"Mahmoud","family":"Elhefnawi","sequence":"additional","affiliation":[{"name":"Biomedical Informatics and Chemo-Informatics Group Leader, Centre of Excellence for Medical Research, Informatics and System Dept, National Research Centre (NRC), Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1080\/01913123.2017.1316330","volume":"41","author":"Mansy S.S.","year":"2017","unstructured":"Mansy S.S.; El-Ahwany E.; Mahmoud S.; Potential ultrastructure predicting factors for hepatocellular carcinoma in HCV infected patients. 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