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In this study, we\nconducted TGF-\u03b21-based CRISPR\/Cas9 gene therapy for RILI.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objectives:<\/jats:title>\n<jats:p>Mouse lungs were irradiated with a single-dose\nof 20-Gy gamma rays followed by intravenous administration of Ad-CRISPR-TGF-\u03b21 or Ad- CRISPR-Null. <\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Haematoxylin and eosin staining, as well as Masson staining were performed to observe lung morphology. Albumin and\nIgM concentrations in bronchoalveolar lavage fluid were measured by ELISA. Cytokine levels were measured using ELISA\nand\/or real-time PCR with terminal deoxynucleotidyl transferase mediated nick-end labelling.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p> Ad-CRISPR-TGF\u03b21 improved histopathological and biochemical markers of lung injury, reduced secretion and expression of inflammatory\ncytokines, and inhibited progression of fibrosis. Importantly, the SK1\/S1P axis\u2014which is known to play a key role via S1P1\nin TGF-\u03b21-dependent S1PR pattern remodelling\u2014is responsible for promoting fibrosis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p> Our results indicate\nnovel insights for RILI therapy.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566523220666201230100523","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T04:00:50Z","timestamp":1609387250000},"page":"59-65","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["TGF-\u03b21-based CRISPR\/Cas9 Gene Therapy Attenuates Radiation-induced\nLung Injury"],"prefix":"10.2174","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3833-7958","authenticated-orcid":true,"given":"Shuai","family":"Zhen","sequence":"first","affiliation":[{"name":"Center for Translational Medicine, The First Affiliated Hospital of Xi\\'an Jiaotong University, Xi\\'an, Shaanxi, 710061,\nPR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4772-8579","authenticated-orcid":true,"given":"Rong","family":"Qiang","sequence":"additional","affiliation":[{"name":"Medical Heredity Research Center, Northwest Women\\'s and Children\\'s Hospital, Shaanxi, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3129-8922","authenticated-orcid":true,"given":"Jiaojiao","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, the First Affiliated Hospital of Xi\\'an Jiaotong University, Xi\\'an, Shaanxi,\n710061, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqian","family":"Tuo","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, the First Affiliated Hospital of Xi\\'an Jiaotong University, Xi\\'an, Shaanxi,\n710061, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2910-2102","authenticated-orcid":true,"given":"Xiling","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, the First Affiliated Hospital of Xi\\'an Jiaotong University, Xi\\'an, Shaanxi,\n710061, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine, the First Affiliated Hospital of Xi\\'an Jiaotong University, Xi\\'an, Shaanxi,\n710061, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.radonc.2014.03.001","volume":"111","author":"Zhang J.","year":"2014","unstructured":"Zhang J.; Li B.; Ding X.; Sun M.; Li H.; Yang M.; Zhou C.; Yu H.; Liu H.; Yu G.; Genetic variants in inducible nitric oxide synthase gene are associated with the risk of radiation-induced lung injury in lung cancer patients receiving definitive thoracic radiation. 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