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The emergence of multi-drug resistant strains has\nmade it harder to treat and combat infectious diseases with available conventional antibiotics. There\nare currently few effective therapeutic regimens for the successful prevention of infections caused by\ndrug-resistant microbes. Various alternative strategies used in the recent past to decrease and limit antibiotic\nresistance in pathogens include bacteriophages, vaccines, anti-biofilm peptides, and antimicrobial\npeptides. However, in this review, we focus on the novel and robust molecular approach of antisense\nRNA (asRNA) technology and the clustered regulatory interspaced short palindromic repeat\n(CRISPR)-based antibiotic therapy, which can be exploited to selectively eradicate the drug-resistant\nbacterial strain in a sequence-specific fashion establishing opportunities in the treatment of multi-drug\nresistant related infections.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566523221666210419084836","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T05:10:50Z","timestamp":1618895450000},"page":"79-88","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Quest to the Therapeutic Arsenal: Novel Strategies to Combat Multidrug-\nresistant Bacteria"],"prefix":"10.2174","volume":"22","author":[{"given":"Priyanka","family":"Ashwath","sequence":"first","affiliation":[{"name":"Nitte (Deemed to be University), Nitte University Centre for Science Education &amp; Research (NUCSER), Mangaluru,\nIndia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akhila Dharnappa","family":"Sannejal","sequence":"additional","affiliation":[{"name":"Nitte (Deemed to be University), Nitte University Centre for Science Education &amp; Research (NUCSER), Mangaluru,\nIndia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1128\/AEM.02912-13","volume":"80","author":"Palliyil S.","year":"2014","unstructured":"Palliyil S.; Downham C.; Broadbent I.; Charlton K.; Porter A.J.; High-sensitivity monoclonal antibodies specific for homoserine lactones protect mice from lethal Pseudomonas aeruginosa infections. 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International Journal of Innovative Technology and Exploring Engineering 2020,3"},{"key":"ref=86","doi-asserted-by":"publisher","first-page":"203","DOI":"10.5483\/BMBRep.2014.47.4.040","volume":"47","author":"Gong F.Y.","year":"2014","unstructured":"Gong F.Y.; Zhang D.Y.; Zhang J.G.; siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa. BMB Rep 2014,47(4),203-208","journal-title":"BMB Rep"},{"key":"ref=87","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1089\/cmb.2019.0156","volume":"27","author":"Mohanty P.S.","year":"2020","unstructured":"Mohanty P.S.; Sharma S.; Naaz F.; Kumar D.; Raikwar A.; Patil S.A.; Inhibition of Mycobacterium tuberculosis tRNA-Ligases Using siRNA-Based Gene Silencing Method: A Computational Approach. 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