{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:48:13Z","timestamp":1761598093412},"reference-count":29,"publisher":"Bentham Science Publishers Ltd.","issue":"8","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMM"],"published-print":{"date-parts":[[2019,9,5]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Central pro-inflammatory cytokine (PIC) signal is involved in\nneurological deficits after transient global ischemia induced by cardiac arrest (CA). The\npresent study was to examine if blocking acid sensing ion channels (ASICs) using\namiloride in the Central Nervous System can alleviate neurological deficits after the\ninduction of CA and further examine the participation of PIC signal in the hippocampus\nfor the effects of amiloride.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>CA was induced by asphyxia and then cardiopulmonary resuscitation was\nperformed in rats. Western blot analysis and ELISA were used to determine the protein\nexpression of ASIC subunit ASIC1 in the hippocampus, and the levels of PICs. As\nnoted, it is unlikely that this procedure is clinically used although amiloride and other\npharmacological agents were given into the brain in this study.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>CA increased ASIC1 in the hippocampus of rats in comparison with control\nanimals. This was associated with the increase in IL-1\u03b2, IL-6 and TNF-\u03b1 together with\nCaspase-3 and Caspase-9. The administration of amiloride into the lateral ventricle\nattenuated the upregulation of Caspase-3\/Caspase-9 and this further alleviated\nneurological severity score and brain edema. Inhibition of central IL-6 and TNF-\u03b1 also\ndecreased ASIC1 in the hippocampus of CA rats.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Transient global ischemia induced by CA amplifies ASIC1a in the\nhippocampus likely via PIC signal. Amiloride administered into the Central Nervous\nSystem plays a neuroprotective role in the process of global ischemia. Thus, targeting\nASICs (i.e., ASIC1a) is suggested for the treatment and improvement of CA-evoked\nglobal cerebral ischemia.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566524019666190704100444","type":"journal-article","created":{"date-parts":[[2019,7,5]],"date-time":"2019-07-05T07:35:18Z","timestamp":1562312118000},"page":"597-604","update-policy":"http:\/\/dx.doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Amiloride Alleviates Neurological Deficits Following Transient Global Ischemia and Engagement of Central IL-6 and TNF-\u03b1 Signal"],"prefix":"10.2174","volume":"19","author":[{"given":"Li","family":"Pang","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouqin","family":"Ji","sequence":"additional","affiliation":[{"name":"Jiutai District People's Hospital of Changchun, Changchun, Jilin 130500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihong","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.resuscitation.2008.09.017","volume":"79","author":"Nolan J.P.","year":"2008","unstructured":"Nolan J.P.; Neumar R.W.; Adrie C.; Post-cardiac arrest syndrome: Epidemiology, pathophysiology, treatment, and prognostication. 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