{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T07:22:46Z","timestamp":1747207366477,"version":"3.40.5"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"8","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMM"],"published-print":{"date-parts":[[2020,12,29]]},"abstract":"<jats:sec>\n<jats:title>Purpose:<\/jats:title>\n<jats:p>A small molecular compound, aminooxy-acetic acid (AOA), has\nbeen shown to modulate experimental autoimmune encephalomyelitis (EAE). The\ncurrent study was designed to investigate whether AOA has a similar effect on the\ndevelopment of experimental autoimmune uveitis (EAU) and to further explore\nunderlying mechanisms of this drug.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>EAU was induced in C57BL\/6J mice by immunization with interphotoreceptor\nretinoid-binding protein peptide 651-670 (IRBP 651-670). AOA (500\u03bcg or 750\u03bcg) or\nvehicle was administered by intraperitoneal injection from day 10 to 14 after EAU\ninduction. The severity was assessed by clinical and histological scores. The integrity of\nthe blood retinal barrier was detected with Evans Blue. Frequencies of splenic Th1,\nTh17 and Foxp3+ Treg cells were examined by flow cytometry. The production of\ncytokines was tested by ELISA. The mRNA expression of IL-17, IFN-\u03b3 and IL-10 was\ndetected by RT-PCR. The expression of p-Stat1 and NF-\u03baB was detected by Western\nBlotting.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>AOA was found to markedly inhibit the severity of EAU, as determined by\nclinical and histopathological examinations. AOA can relieve the leakage of blood retinal\nbarrier (BRB). Functional studies found a decreased frequency of Th1 and Th17 cells\nand an increased frequency of Treg cells in EAU mice as compared with controls.\nFurther studies showed that AOA not only downregulated the production of the proinflammatory\ncytokines including IFN-\u03b3 and IL-17 but also upregulated the expression of\nan anti-inflammatory cytokine such as IL-10, which might be caused by inhibiting the\nexpressions of p-Stat1 and NF-\u03baB.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>This study shows that AOA inhibits the severity and development of EAU\nby modulating the balance between regulatory and pathogenic lymphocyte subsets.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566524020666200211112219","type":"journal-article","created":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T14:51:53Z","timestamp":1581432713000},"page":"624-632","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Aminooxy-Acetic Acid Inhibits Experimental Autoimmune Uveitis by Modulating the Balance between Effector and Regulatory Lymphocyte Subsets"],"prefix":"10.2174","volume":"20","author":[{"given":"Suyin","family":"Mei","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yike","family":"Huang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongren","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinjin","family":"Dai","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Wu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aize","family":"Kijlstra","sequence":"additional","affiliation":[{"name":"University Eye Clinic Maastricht, Maastricht, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peizeng","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8371-7223","authenticated-orcid":true,"given":"Shengping","family":"Hou","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, Chongqing Key Laboratory of Ophthalmology, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Current Molecular 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