{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:55:00Z","timestamp":1762102500617},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"10","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMM"],"published-print":{"date-parts":[[2021,2,3]]},"abstract":"<jats:sec>\n<jats:title>Aims:<\/jats:title>\n<jats:p>Application of capillary electrophoresis with laser induced fluorescence\ndetection (CE-LIF) to identify the N-glycosylation structures of serum and saliva IgA from\nhealthy controls and patients with malignant hematological diseases having cytostatic\ntreatment induced mild oral mucosal lesions.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Altered N-glycosylation of body fluid glycoproteins can be an effective\nindicator of most inflammatory processes. Immunoglobulin A (IgA) is the second highest\nabundant immunoglobulin and has a major role in the immune-defense against potential\npathogen attacks. While IgA is abundant in serum, secretory immunoglobulin A (sIgA) is\none of the most prevalent proteins in mucosal surfaces, such as in saliva.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>Our aim was to investigate the changes of IgA glycosylation in serum and\nsaliva as a response to an administered cytostatic treatment in patients with malignant\nhematological disorders.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>Capillary electrophoresis with laser induced fluorescent detection (CE-LIF)\nwas used to analyze the N-glycosylation profiles of Z(IgA1) partitioned immunoglobulin A\nin pooled serum and saliva of 10 control subjects and 8 patients with malignant\nhematological diseases having cytostatic treatment induced mild oral mucosal lesions.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Eight of 31 and four of 38 N-glycans in serum and saliva, respectively, showed\nsignificant (p&lt;0.05) differences upon comparison to the control group. Thirteen glycans\nwere present in the saliva but not in the serum, on the other hand, six structures were\nfound in the serum samples not present in the saliva.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p> The developed Z(IgA1) partitioning and the high resolution CE-LIF based\nglyocoanalytical methods provided an efficient and sensitive workflow to detect and\nmonitor IgA glycosylation alterations in serum and saliva with the scope for widespread\nmolecular medicinal use.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566524020666200413114151","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T18:53:31Z","timestamp":1586804011000},"page":"781-788","update-policy":"http:\/\/dx.doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["N-glycomic Analysis of Z(IgA1) Partitioned Serum and Salivary Immunoglobulin A by Capillary Electrophoresis"],"prefix":"10.2174","volume":"20","author":[{"given":"Brigitta","family":"Meszaros","sequence":"first","affiliation":[{"name":"Horvath Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School for Medicine, Faculty of Medicine, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsanna","family":"Kovacs","sequence":"additional","affiliation":[{"name":"Horvath Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School for Medicine, Faculty of Medicine, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eniko","family":"Gebri","sequence":"additional","affiliation":[{"name":"Department of Dentoalveolar Surgery and Dental Outpatient Care, Faculty of Dentistry, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajnalka","family":"Jankovics","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferenc","family":"Vonderviszt","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila","family":"Kiss","sequence":"additional","affiliation":[{"name":"Department of Hematopoietic Transplantation Center, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Simon","sequence":"additional","affiliation":[{"name":"Horvath Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School for Medicine, Faculty of Medicine, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarolta","family":"Botka","sequence":"additional","affiliation":[{"name":"BotFog Kft, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tibor","family":"Hortobagyi","sequence":"additional","affiliation":[{"name":"Cerebrovascular and Neurodegenerative Research Group, Department of Neurology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andras","family":"Guttman","sequence":"additional","affiliation":[{"name":"Horvath Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School for Medicine, Faculty of Medicine, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Current Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/180847","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/180847\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/180847","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T09:31:15Z","timestamp":1612776675000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/180847\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,3]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,2,3]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1566524020666200413114151","relation":{},"ISSN":["1566-5240"],"issn-type":[{"value":"1566-5240","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2,3]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-19","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication 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