{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T20:41:24Z","timestamp":1648672884152},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"10","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMM"],"published-print":{"date-parts":[[2021,2,3]]},"abstract":"<jats:sec>\n<jats:title \/>\n<jats:p>The utilization of N-glycan profiling recently gained high importance in\nfundamental biomedical and applied clinical research. However, for the time being, no\nglycan biomarker has been approved for clinical diagnosis by the regulatory agencies\ndue to the lack of verifications on large patient cohorts and suitable analytical\ntechnologies. In this paper, the effect of human blood sample handling was studied prior\nto N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity\nfluorescence detection. Special attention was paid to the preservation of sialylated\nstructures because of their important clinical - biological relevance. Our results\nsuggested that it is adequate to refrigerate and store the collected total blood samples\nprior to analysis to obtain unbiased results. Furthermore, we report on the good practice\nof serum sample handling in order to prevent decomposition of the sialylated structures.\nOur findings may promote procedure standardization and easier clinical translation of\ndiagnostic N-glycosylation profiling in molecular medicinal applications.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566524020666201230094722","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T04:00:50Z","timestamp":1609387250000},"page":"840-846","update-policy":"http:\/\/dx.doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples"],"prefix":"10.2174","volume":"20","author":[{"given":"Rebeka","family":"Torok","sequence":"first","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Farkas","sequence":"additional","affiliation":[{"name":"Horvath Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andras","family":"Guttman","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabor","family":"Jarvas","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Current Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/189622","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/189622\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/189622","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T09:31:22Z","timestamp":1612776682000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/189622\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,3]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,2,3]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1566524020666201230094722","relation":{},"ISSN":["1566-5240"],"issn-type":[{"value":"1566-5240","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2,3]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2020-09-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-09","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}