{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:52:43Z","timestamp":1785804763283,"version":"3.56.0"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"6","funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, India","doi-asserted-by":"publisher","award":["BT\/PR15014\/BRB\/10\/1481\/2016"],"award-info":[{"award-number":["BT\/PR15014\/BRB\/10\/1481\/2016"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMM"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>The dynamic changes that bone undergoes during the\nensemble of remodeling are administered by vital factors like Runx2 (a bone\ntranscription factor) and matrix metalloproteinases (MMPs).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Aims:<\/jats:title>\n<jats:p>Parathyroid hormone (PTH), an FDA approved drug for bone-related ailments,\nwas seen to stimulate MMP-13 expression via Runx2 to ultimately aid in the bone\nremodeling process. MicroRNAs (miRNAs) have been shown to play a major role in\ncontrolling bone metabolism, and the use of miRNAs has recently become promising\ntherapeutic avenues for the treatment of many diseases, including bone disorders. Thus,\nin this study, we attempted to investigate and evaluate the expression of MMP-13 via a\nmiRNA profile targeting Runx2 under PTH-regulation in rat osteoblastic cells.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>PTH stimulated the expression of MMP-13 mRNA significantly at 4 h in rat\nosteoblastic cells (UMR106-01). Runx2 was required for PTH-stimulation of MMP-13\nexpression, in silico scrutiny generated 14 unique miRNAs targeting Runx2, and among\nthese miRNAs, miR-290 was significantly downregulated by PTH-treatment in UMR106-\n01 cells and in rat primary osteoblasts.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Overexpression of miR-290 decreased the expression of Runx2, the binding of\nRunx2 at the MMP-13 promoter, and the expression of MMP-13 mRNA in PTH-treated\nUMR106-01 cells. A dual luciferase reporter assay identified the direct targeting of\nRunx2 mRNA by miR-290 in these cells.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Our findings indicate that the PTH-responsive miR-290 regulated Runx2-\nmediated MMP-13 expression in rat osteoblastic cells, suggesting miR-290 as a\nmolecular marker or target in bone and bone-related diseases.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1566524021666210830093232","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T09:28:11Z","timestamp":1630402091000},"page":"549-561","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Parathyroid Hormone-regulation of Runx2 by MiR-290 for Matrix\nMetalloproteinase-13 Expression in Rat Osteoblastic Cells"],"prefix":"10.2174","volume":"22","author":[{"given":"N.","family":"Akshaya","sequence":"first","affiliation":[{"name":"Department of Biotechnology, College of Engineering and Technology, SRM Institute of Science and\nTechnology, Kattankulathur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Srinaath","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Engineering and Technology, SRM Institute of Science and\nTechnology, Kattankulathur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rohini","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Engineering and Technology, SRM Institute of Science and\nTechnology, Kattankulathur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Ilangovan","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Dr. A.L.M. PG Institute of\nBasic Medical Sciences, University of Madras, Taramani, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3713-1920","authenticated-orcid":true,"given":"N.","family":"Selvamurugan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Engineering and Technology, SRM Institute of Science and\nTechnology, Kattankulathur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","container-title":["Current Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/1566524021666210830093232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/195921\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/1566524021666210830093232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T13:05:47Z","timestamp":1653656747000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/195921\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1566524021666210830093232","relation":{},"ISSN":["1566-5240"],"issn-type":[{"value":"1566-5240","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2021-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-16","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}