{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:53:25Z","timestamp":1793109205416,"version":"4.5.0"},"reference-count":57,"publisher":"Bentham Science Publishers Ltd.","issue":"2","license":[{"start":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T00:00:00Z","timestamp":1544745600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CDD"],"published-print":{"date-parts":[[2018,12,14]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Delivery of foreign cargoes into cells is of great value for bioengineering research and therapeutic applications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>In this study, we proposed and established a carrier-free gene delivery platform utilizing staggered herringbone channel and silicon nanoneedle array, to achieve high-throughput in vitro gene transfection. &lt;\/P&gt;&lt;P&gt; Methods: With this microchip, fluidic micro vortices could be induced by the staggered-herringboneshaped grooves within the channel, which increased the contact frequency of the cells with the channel substrate. Transient disruptions on the cell membrane were well established by the nanoneedle array on the substrate. &lt;\/P&gt;&lt;P&gt; Result: Compared to the conventional nanoneedle-based delivery system, proposed microfluidic chip achieved flow-through treatment with high gene transfection efficiency (higher than 20%) and ideal cell viability (higher than 95%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>It provides a continuous processing environment that can satisfy the transfection requirement of large amounts of biological molecules, showing high potential and promising prospect for both basic research and clinical application.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2174\/1567201815666181017095044","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T04:09:55Z","timestamp":1539835795000},"page":"164-170","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Continuous Vector-free Gene Transfer with a Novel Microfluidic Chip and Nanoneedle Array"],"prefix":"10.2174","volume":"16","author":[{"given":"Dong","family":"Huang","sequence":"first","affiliation":[{"name":"Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro\/Nano Fabrication, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro\/Nano Fabrication, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhui","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro\/Nano Fabrication, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro\/Nano Fabrication, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro\/Nano Fabrication, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Hua","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiong","family":"Li","sequence":"additional","affiliation":[{"name":"Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotheranotics, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulin","family":"Deng","sequence":"additional","affiliation":[{"name":"Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotheranotics, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine; Institute of Microelectronics, National Key Laboratory of Science and Technology on Micro\/Nano Fabrication, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyu","family":"Huang","sequence":"additional","affiliation":[{"name":"Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotheranotics, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"crossref","first-page":"6111","DOI":"10.1073\/pnas.0807883106","volume":"106","author":"McNaughton B.R.","year":"2009","unstructured":"McNaughton BR, Cronican JJ, Thompson DB, Liu DR. Proc Natl Acad Sci USA, Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.,, 2009, 106,, 6111-6116,","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref=2","doi-asserted-by":"crossref","first-page":"6916","DOI":"10.1021\/acs.nanolett.6b02915","volume":"16","author":"Zhou J.","year":"2016","unstructured":"Zhou J, Wu Y, Wang C, Cheng Q, Han S, Wang X, Zhang J, Deng L, Zhao D, Du L, Cao H, Liang Z, Huang Y, Dong A. Nano Lett, pH-sensitive nanomicelles for high-efficiency siRNA delivery in vitro and in vivo: An insight into the design of polycations with robust cytosolic release.,, 2016, 16,, 6916-6923,","journal-title":"Nano Lett"},{"key":"ref=3","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.cmet.2018.03.004","volume":"27","author":"Crooke S.T.","year":"2018","unstructured":"Crooke ST, Witztum JL, Bennett CF, Baker BF. Cell Metab, RNA-targeted therapeutics.,, 2018, 27,, 714-739,","journal-title":"Cell Metab"},{"key":"ref=4","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1039\/C0CS00003E","volume":"40","author":"Chou L.Y.","year":"2011","unstructured":"Chou LY, Ming K, Chan WC. Chem Soc Rev, Strategies for the intracellular delivery of nanoparticles.,, 2011, 40,, 233-245,","journal-title":"Chem Soc Rev"},{"key":"ref=5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/nrd2399","volume":"7","author":"Leader B.","year":"2008","unstructured":"Leader B, Baca QJ, Golan DE. Nat Rev Drug Discov, Protein therapeutics: A summary and pharmacological classification.,, 2008, 7,, 21-39,","journal-title":"Nat Rev Drug Discov"},{"key":"ref=6","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.stem.2009.05.005","volume":"4","author":"Kim D.","year":"2009","unstructured":"Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, Ko S, Yang E, Cha KY, Lanza R, Kim KS. Cell Stem Cell, Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.,, 2009, 4,, 472-476,","journal-title":"Cell Stem Cell"},{"key":"ref=7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.addr.2017.07.021","volume":"115","author":"Zhou Z.","year":"2017","unstructured":"Zhou Z, Liu X, Zhu D, Wang Y, Zhang Z, Zhou X, Qiu N, Chen X, Shen Y. Adv Drug Deliv Rev, Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration.,, 2017, 115,, 115-154,","journal-title":"Adv Drug Deliv Rev"},{"key":"ref=8","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.1166\/jbn.2014.2044","volume":"10","author":"Xu H.","year":"2014","unstructured":"Xu H, Li Z, Si J. J Biomed Nanotechnol, Nanocarriers in gene therapy: A review.,, 2014, 10,, 3483-3507,","journal-title":"J Biomed Nanotechnol"},{"key":"ref=9","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.omtn.2016.12.003","volume":"6","author":"Huang Y.","year":"2017","unstructured":"Huang Y. Mol Ther Nucleic Acids, Preclinical and clinical advances of GalNAc-decorated nucleic acid therapeutics.,, 2017, 6,, 116-132,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=10","first-page":"501","volume":"42","author":"Huang Y.Y.","year":"2015","unstructured":"Huang YY, Liang ZC. Prog Biochem Biophys, Asialoglycoprotein receptor and its application in liver-targeted drug delivery.,, 2015, 42,, 501-510,","journal-title":"Prog Biochem Biophys"},{"key":"ref=11","doi-asserted-by":"crossref","first-page":"224","DOI":"10.2174\/1567201813666160811145855","volume":"14","author":"Cavallaro G.","year":"2017","unstructured":"Cavallaro G, Sardo C, Scialabba C, Licciardi M, Giammona G. Curr Drug Deliv, Smart inulin-based polycationic nanodevices for siRNA delivery.,, 2017, 14,, 224-230,","journal-title":"Curr Drug Deliv"},{"key":"ref=12","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.1016\/j.biomaterials.2013.01.043","volume":"34","author":"Cheng Q.","year":"2013","unstructured":"Cheng Q, Huang Y, Zheng H, Wei T, Zheng S, Huo S, Wang X, Du Q, Zhang X, Zhang HY, Liang XJ, Wang C, Tang R, Liang Z. Biomaterials, The effect of guanidinylation of PEGylated poly(2-aminoethyl methacrylate) on the systemic delivery of siRNA.,, 2013, 34,, 3120-3131,","journal-title":"Biomaterials"},{"key":"ref=13","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nrd.2016.280","volume":"16","author":"Yin H.","year":"2017","unstructured":"Yin H, Kauffman KJ, Anderson DG. Nat Rev Drug Discov, Delivery technologies for genome editing.,, 2017, 16,, 387-399,","journal-title":"Nat Rev Drug Discov"},{"key":"ref=14","doi-asserted-by":"crossref","first-page":"334","DOI":"10.2174\/1567201813666160108114430","volume":"14","author":"Lin L.","year":"2017","unstructured":"Lin L, Li X, Yang Y, Jing L, Yue X, Chen X, Dai Z. Curr Drug Deliv, Chitosan functionalized CuS nanoparticles boots gene transfection via photothermal effect.,, 2017, 14,, 334-341,","journal-title":"Curr Drug Deliv"},{"key":"ref=15","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/83324","volume":"7","author":"Kay M.A.","year":"2001","unstructured":"Kay MA, Glorioso JC, Naldini L. Nat Med, Viral vectors for gene therapy: The art of turning infectious agents into vehicles of therapeutics.,, 2001, 7,, 33-40,","journal-title":"Nat Med"},{"key":"ref=16","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/nrg1066","volume":"4","author":"Thomas C.E.","year":"2003","unstructured":"Thomas CE, Ehrhardt A, Kay MA. Nat Rev Genet, Progress and problems with the use of viral vectors for gene therapy.,, 2003, 4,, 346-358,","journal-title":"Nat Rev Genet"},{"key":"ref=17","doi-asserted-by":"crossref","first-page":"723","DOI":"10.2174\/1389200219666171207120643","volume":"19","author":"Hu J.","year":"2018","unstructured":"Hu J, Sheng Y, Shi J, Yu B, Yu Z, Liao G. Curr Drug Metab, Long circulating polymeric nanoparticles for gene\/drug delivery.,, 2018, 19,, 723-738,","journal-title":"Curr Drug Metab"},{"key":"ref=18","doi-asserted-by":"crossref","first-page":"6518","DOI":"10.1093\/nar\/gkw236","volume":"44","author":"Juliano R.L.","year":"2016","unstructured":"Juliano RL. Nucleic Acids Res, The delivery of therapeutic oligonucleotides.,, 2016, 44,, 6518-6548,","journal-title":"Nucleic Acids Res"},{"key":"ref=19","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/nmeth.3357","volume":"12","author":"Wu Y.C.","year":"2015","unstructured":"Wu YC, Wu TH, Clemens DL, Lee BY, Wen X, Horwitz MA, Teitell MA, Chiou PY. Nat Methods, Massively parallel delivery of large cargo into mammalian cells with light pulses.,, 2015, 12,, 439-444,","journal-title":"Nat Methods"},{"key":"ref=20","doi-asserted-by":"crossref","first-page":"5326","DOI":"10.1021\/acs.nanolett.6b00902","volume":"16","author":"Gallego-Perez D.","year":"2016","unstructured":"Gallego-Perez D, Chang L, Shi J, Ma J, Kim SH, Zhao X, Malkoc V, Wang X, Minata M, Kwak KJ, Wu Y, Lafyatis GP, Lu W, Hansford DJ, Nakano I, Lee LJ. Nano Lett, On-chip clonal analysis of glioma-stem-cell motility and therapy resistance.,, 2016, 16,, 5326-5332,","journal-title":"Nano Lett"},{"key":"ref=21","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1038\/nnano.2017.134","volume":"12","author":"Gallego-Perez D.","year":"2017","unstructured":"Gallego-Perez D, Pal D, Ghatak S, Malkoc V, Higuita-Castro N, Gnyawali S, Chang L, Liao WC, Shi J, Sinha M, Singh K, Steen E, Sunyecz A, Stewart R, Moore J, Ziebro T, Northcutt RG, Homsy M, Bertani P, Lu W, Roy S, Khanna S, Rink C, Sundaresan VB, Otero JJ, Lee LJ, Sen CK. Nat Nanotechnol, Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue.,, 2017, 12,, 974-979,","journal-title":"Nat Nanotechnol"},{"key":"ref=22","doi-asserted-by":"crossref","first-page":"4047","DOI":"10.1039\/C6LC00840B","volume":"16","author":"Chang L.","year":"2016","unstructured":"Chang L, Li L, Shi J, Sheng Y, Lu W, Gallego-Perez D, Lee LJ. Lab Chip, Micro-\/nanoscale electroporation.,, 2016, 16,, 4047-4062,","journal-title":"Lab Chip"},{"key":"ref=23","doi-asserted-by":"crossref","first-page":"4093","DOI":"10.1039\/C4LC00800F","volume":"14","author":"Wei Z.","year":"2014","unstructured":"Wei Z, Zheng S, Wang R, Bu X, Ma H, Wu Y, Zhu L, Hu Z, Liang Z, Li Z. Lab Chip, A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery.,, 2014, 14,, 4093-4102,","journal-title":"Lab Chip"},{"key":"ref=24","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1039\/C0LC00195C","volume":"11","author":"Huang H.","year":"2011","unstructured":"Huang H, Wei Z, Huang Y, Zhao D, Zheng L, Cai T, Wu M, Wang W, Ding X, Zhou Z, Du Q, Li Z, Liang Z. Lab Chip, An efficient and high-throughput electroporation microchip applicable for siRNA delivery.,, 2011, 11,, 163-172,","journal-title":"Lab Chip"},{"key":"ref=25","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jconrel.2006.02.007","volume":"112","author":"van Wamel A.","year":"2006","unstructured":"van Wamel A, Kooiman K, Harteveld M, Emmer M, ten Cate FJ, Versluis M, de Jong N. J Control Release, Vibrating microbubbles poking individual cells: Drug transfer into cells via sonoporation.,, 2006, 112,, 149-155,","journal-title":"J Control Release"},{"key":"ref=26","first-page":"46","volume":"60","author":"Escoffre J.M.","year":"2013","unstructured":"Escoffre JM, Novell A, Piron J, Zeghimi A, Doinikov A, Bouakaz A. IEEE Trans Ultrason Ferroelectr Freq Control, Microbubble attenuation and destruction: Are they involved in sonoporation efficiency?,, 2013, 60,, 46-52,","journal-title":"IEEE Trans Ultrason Ferroelectr Freq Control"},{"key":"ref=27","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/418290a","volume":"418","author":"Tirlapur U.K.","year":"2002","unstructured":"Tirlapur UK, Konig K. Nature, Cell biology - Targeted transfection by femtosecond laser.,, 2002, 418,, 290-291,","journal-title":"Nature"},{"key":"ref=28","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1039\/C3LC51394G","volume":"14","author":"Fan Q.","year":"2014","unstructured":"Fan Q, Hu W, Ohta AT. Lab Chip, Laser-induced microbubble poration of localized single cells.,, 2014, 14,, 1572-1578,","journal-title":"Lab Chip"},{"key":"ref=29","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1073\/pnas.1218705110","volume":"110","author":"Sharei A.","year":"2013","unstructured":"Sharei A, Zoldan J, Adamo A, Sim WY, Cho N, Jackson E, Mao S, Schneider S, Han MJ, Lytton-Jean A, Basto PA, Jhunjhunwala S, Lee J, Heller DA, Kang JW, Hartoularos GC, Kim KS, Anderson DG, Langer R, Jensen KF. Proc Natl Acad Sci USA, A vector-free microfluidic platform for intracellular delivery.,, 2013, 110,, 2082-2087,","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref=30","doi-asserted-by":"crossref","first-page":"6498","DOI":"10.1021\/nl3042917","volume":"12","author":"Shalek A.K.","year":"2012","unstructured":"Shalek AK, Gaublomme JT, Wang L, Yosef N, Chevrier N, Andersen MS, Robinson JT, Pochet N, Neuberg D, Gertner RS, Amit I, Brown JR, Hacohen N, Regev A, Wu CJ, Park H. Nano Lett, Nanowire-mediated delivery enables functional interrogation of primary immune cells: Application to the analysis of chronic lymphocytic leukemia.,, 2012, 12,, 6498-6504,","journal-title":"Nano Lett"},{"key":"ref=31","doi-asserted-by":"crossref","first-page":"6002","DOI":"10.1021\/nl403201a","volume":"13","author":"Xie X.","year":"2013","unstructured":"Xie X, Xu AM, Angle MR, Tayebi N, Verma P, Melosh NA. Nano Lett, Mechanical model of vertical nanowire cell penetration.,, 2013, 13,, 6002-6008,","journal-title":"Nano Lett"},{"key":"ref=32","doi-asserted-by":"crossref","first-page":"7058","DOI":"10.1021\/acs.nanolett.5b03126","volume":"15","author":"Wang Z.","year":"2015","unstructured":"Wang Z, Yang Y, Xu Z, Wang Y, Zhang W, Shi P. Nano Lett, Interrogation of cellular innate immunity by diamond-nanoneedle-assisted intracellular molecular fishing.,, 2015, 15,, 7058-7063,","journal-title":"Nano Lett"},{"key":"ref=33","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1021\/am800219r","volume":"1","author":"Jiang K.","year":"2009","unstructured":"Jiang K, Fan D, Belabassi Y, Akkaraju G, Montchamp JL, Coffer JL. ACS Appl Mater Interfaces, Medicinal surface modification of silicon nanowires: Impact on calcification and stromal cell proliferation.,, 2009, 1,, 266-269,","journal-title":"ACS Appl Mater Interfaces"},{"key":"ref=34","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1073\/pnas.0909350107","volume":"107","author":"Shalek A.K.","year":"2010","unstructured":"Shalek AK, Robinson JT, Karp ES, Lee JS, Ahn DR, Yoon MH, Sutton A, Jorgolli M, Gertner RS, Gujral TS, MacBeath G, Yang EG, Park H. Proc Natl Acad Sci USA, Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells.,, 2010, 107,, 1870-1875,","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref=35","doi-asserted-by":"crossref","first-page":"5500","DOI":"10.1021\/acsnano.5b01490","volume":"9","author":"Chiappini C.","year":"2015","unstructured":"Chiappini C, Martinez JO, De Rosa E, Almeida CS, Tasciotti E, Stevens MM. ACS Nano, Biodegradable nanoneedles for localized delivery of nanoparticles in vivo: Exploring the biointerface.,, 2015, 9,, 5500-5509,","journal-title":"ACS Nano"},{"key":"ref=36","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1038\/nmat4249","volume":"14","author":"Chiappini C.","year":"2015","unstructured":"Chiappini C, De Rosa E, Martinez JO, Liu X, Steele J, Stevens MM, Tasciotti E. Nat Mater, Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.,, 2015, 14,, 532-539,","journal-title":"Nat Mater"},{"key":"ref=37","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1002\/adhm.201200362","volume":"2","author":"Chen X.","year":"2013","unstructured":"Chen X, Zhu G, Yang Y, Wang B, Yan L, Zhang KY, Lo KK, Zhang W. Adv Healthc Mater, A diamond nanoneedle array for potential high-throughput intracellular delivery.,, 2013, 2,, 1103-1107,","journal-title":"Adv Healthc Mater"},{"key":"ref=38","doi-asserted-by":"crossref","first-page":"4466","DOI":"10.1038\/ncomms5466","volume":"5","author":"Wang Y.","year":"2014","unstructured":"Wang Y, Yang Y, Yan L, Kwok SY, Li W, Wang Z, Zhu X, Zhu G, Zhang W, Chen X, Shi P. Nat Commun, Poking cells for efficient vector-free intracellular delivery.,, 2014, 5,, 4466-,","journal-title":"Nat Commun"},{"key":"ref=39","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1126\/science.1066238","volume":"295","author":"Stroock A.D.","year":"2002","unstructured":"Stroock AD, Dertinger SK, Ajdari A, Mezic I, Stone HA, Whitesides GM. Science, Chaotic mixer for microchannels.,, 2002, 295,, 647-651,","journal-title":"Science"},{"key":"ref=40","doi-asserted-by":"crossref","first-page":"18392","DOI":"10.1073\/pnas.1012539107","volume":"107","author":"Stott S.L.","year":"2010","unstructured":"Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA, Toner M. Proc Natl Acad Sci USA, Isolation of circulating tumor cells using a microvortex-generating herringbone-chip.,, 2010, 107,, 18392-18397,","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref=41","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1002\/anie.201005853","volume":"50","author":"Wang S.","year":"2011","unstructured":"Wang S, Liu K, Liu J, Yu ZT, Xu X, Zhao L, Lee T, Lee EK, Reiss J, Lee YK, Chung LW, Huang J, Rettig M, Seligson D, Duraiswamy KN, Shen CK, Tseng HR. Angew Chem Int Ed Engl, Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers.,, 2011, 50,, 3084-3088,","journal-title":"Angew Chem Int Ed Engl"},{"key":"ref=42","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/71889","volume":"18","author":"Luo D.","year":"2000","unstructured":"Luo D, Saltzman WM. Nat Biotechnol, Synthetic DNA delivery systems.,, 2000, 18,, 33-37,","journal-title":"Nat Biotechnol"},{"key":"ref=43","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1016\/j.bios.2004.07.020","volume":"20","author":"Obataya I.","year":"2005","unstructured":"Obataya I, Nakamura C, Han S, Nakamura N, Miyake J. Biosens Bioelectron, Mechanical sensing of the penetration of various nanoneedles into a living cell using atomic force microscopy.,, 2005, 20,, 1652-1655,","journal-title":"Biosens Bioelectron"},{"key":"ref=44","doi-asserted-by":"crossref","first-page":"e349","DOI":"10.1038\/mtna.2016.58","volume":"5","author":"Dai W.J.","year":"2016","unstructured":"Dai WJ, Zhu LY, Yan ZY, Xu Y, Wang QL, Lu XJ. Mol Ther Nucleic Acids, CRISPR-Cas9 for in vivo gene therapy: promise and hurdles.,, 2016, 5,, e349-,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=45","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.omtn.2016.12.001","volume":"6","author":"Afzal S.","year":"2017","unstructured":"Afzal S, Wilkening S, von Kalle C, Schmidt M, Fronza R. Mol Ther Nucleic Acids, GENE-IS: Time-efficient and accurate analysis of viral integration events in large-scale gene therapy data.,, 2017, 6,, 133-139,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=46","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.omtn.2017.04.002","volume":"7","author":"El Ashkar S.","year":"2017","unstructured":"El Ashkar S, Van Looveren D, Schenk F, Vranckx LS, Demeulemeester J, De Rijck J, Debyser Z, Modlich U, Gijsbers R. Mol Ther Nucleic Acids, Engineering next-generation BET-Independent MLV Vectors for safer gene therapy.,, 2017, 7,, 231-245,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=47","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.omtn.2017.08.017","volume":"9","author":"Falkenhagen A.","year":"2017","unstructured":"Falkenhagen A, Singh J, Asad S, Leontyev D, Read S, Zuniga-Pflucker JC, Joshi S. Mol Ther Nucleic Acids, Control of HIV infection in vivo using gene therapy with a secreted entry inhibitor.,, 2017, 9,, 132-144,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=48","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.omtn.2018.04.003","volume":"11","author":"Chen C.","year":"2018","unstructured":"Chen C, Yue D, Lei L, Wang H, Lu J, Zhou Y, Liu S, Ding T, Guo M, Xu L. Mol Ther Nucleic Acids, Promoter-operating targeted expression of gene therapy in cancer: Current stage and prospect.,, 2018, 11,, 508-514,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=49","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.omtn.2016.12.010","volume":"6","author":"McCrudden C.M.","year":"2017","unstructured":"McCrudden CM, McBride JW, McCaffrey J, Ali AA, Dunne NJ, Kett VL, Coulter JA, Robson T, McCarthy HO. Mol Ther Nucleic Acids, Systemic RALA\/iNOS nanoparticles: A potent gene therapy for metastatic breast cancer coupled as a biomarker of treatment.,, 2017, 6,, 249-258,","journal-title":"Mol Ther Nucleic Acids"},{"key":"ref=50","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1089\/humc.2016.29010.ind","volume":"27","author":"Schimmer J.","year":"2016","unstructured":"Schimmer J, Breazzano S. Hum Gene Ther Clin Dev, Investor outlook: Rising from the Ashes; GSK\u2019s European Approval of Strimvelis for ADA-SCID.,, 2016, 27,, 57-61,","journal-title":"Hum Gene Ther Clin Dev"},{"key":"ref=51","first-page":"299","volume":"15","author":"Mullard A.","year":"2016","unstructured":"Mullard A. Nat Rev Drug Discov, EMA greenlights second gene therapy.,, 2016, 15,, 299-,","journal-title":"Nat Rev Drug Discov"},{"key":"ref=52","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s13023-018-0791-9","volume":"13","author":"Stirnadel-Farrant H.","year":"2018","unstructured":"Stirnadel-Farrant H, Kudari M, Garman N, Imrie J, Chopra B, Giannelli S, Gabaldo M, Corti A, Zancan S, Aiuti A, Cicalese MP, Batta R, Appleby J, Davinelli M, Ng P. Orphanet J Rare Dis, Gene therapy in rare diseases: The benefits and challenges of developing a patient-centric registry for Strimvelis in ADA-SCID.,, 2018, 13,, 49-,","journal-title":"Orphanet J Rare Dis"},{"key":"ref=53","doi-asserted-by":"crossref","first-page":"4283","DOI":"10.1016\/j.biomaterials.2011.02.034","volume":"32","author":"Guo S.","year":"2011","unstructured":"Guo S, Huang Y, Zhang W, Wang W, Wei T, Lin D, Xing J, Deng L, Du Q, Liang Z, Liang XJ, Dong A. Biomaterials, Ternary complexes of amphiphilic polycaprolactone-graft-poly (N,N-dimethylaminoethyl methacrylate), DNA and polyglutamic acid-graft-poly(ethylene glycol) for gene delivery.,, 2011, 32,, 4283-4292,","journal-title":"Biomaterials"},{"key":"ref=54","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.biomaterials.2010.09.052","volume":"32","author":"Guo S.","year":"2011","unstructured":"Guo S, Huang Y, Wei T, Zhang W, Wang W, Lin D, Zhang X, Kumar A, Du Q, Xing J, Deng L, Liang Z, Wang PC, Dong A, Liang XJ. Biomaterials, Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier.,, 2011, 32,, 879-889,","journal-title":"Biomaterials"},{"key":"ref=55","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.7150\/thno.23438","volume":"8","author":"Huang D.","year":"2018","unstructured":"Huang D, Zhao D, Wang X, Li C, Yang T, Du L, Wei Z, Cheng Q, Cao H, Liang Z, Huang Y, Li Z. Theranostics, Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array.,, 2018, 8,, 2361-2376,","journal-title":"Theranostics"},{"key":"ref=56","doi-asserted-by":"crossref","first-page":"5505","DOI":"10.1021\/nn101638u","volume":"4","author":"Guo S.","year":"2010","unstructured":"Guo S, Huang Y, Jiang Q, Sun Y, Deng L, Liang Z, Du Q, Xing J, Zhao Y, Wang PC, Dong A, Liang XJ. ACS Nano, Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte.,, 2010, 4,, 5505-5511,","journal-title":"ACS Nano"},{"key":"ref=57","doi-asserted-by":"crossref","first-page":"485","DOI":"10.2174\/1566523023347616","volume":"2","author":"Sun J.Y.","year":"2002","unstructured":"Sun JY, Chatterjee S, Wong KK. Curr Gene Ther, Immunogenic issues concerning recombinant adeno-associated virus vectors for gene therapy.,, 2002, 2,, 485-500,","journal-title":"Curr Gene Ther"}],"container-title":["Current Drug Delivery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/166329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T20:33:28Z","timestamp":1775248408000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/166329\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,14]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,12,14]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1567201815666181017095044","relation":{},"ISSN":["1567-2018"],"issn-type":[{"value":"1567-2018","type":"print"}],"subject":[],"published":{"date-parts":[[2018,12,14]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2018-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-24","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}