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Developing a biomarker-based, low-cost, and high-throughput test, readily applicable in clinical laboratories, dramatically impacts the rapid and reliable detection of the disease.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>This study aimed to develop an accurate, sensitive, and reliable screening tool for diagnosing Alzheimer's disease, which can significantly reduce the cost and time of existing methods.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>We have employed a MALDI-TOF-MS-based methodology combined with a microaffinity chromatography enrichment approach using affinity capture resins to determine serum kappa (\u03ba) and lambda (\u03bb) light chain levels in control and patients with AD.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>We observed a statistically significant difference in the kappa light chain over lambda light chain (\u03baLC\/\u03bbLC) ratios between patients with AD and controls (% 95 CI: -0.547 to -0.269, p&lt;0.001). Our method demonstrated higher sensitivity (100.00%) and specificity (71.43%) for discrimination between AD and controls.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>We have developed a high-throughput screening test with a novel sample enrichment method for determining \u03baLC\/\u03bbLC ratios associated with AD diagnosis. Following further validation, we believe our test has a potential for clinical laboratories.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1567205019666220131101334","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T04:46:55Z","timestamp":1643690815000},"page":"84-93","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Kappa\/Lambda light-chain typing in Alzheimer\u2019s Disease"],"prefix":"10.2174","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1415-3708","authenticated-orcid":true,"given":"Zelal Zuhal","family":"Kaya","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8924-4850","authenticated-orcid":true,"given":"Mete 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