{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T19:41:12Z","timestamp":1772221272950,"version":"3.50.1"},"reference-count":19,"publisher":"Bentham Science Publishers Ltd.","issue":"5","funder":[{"DOI":"10.13039\/501100004621","name":"Kerman University of Medical Science, Kerman, Iran","doi-asserted-by":"publisher","award":["104588"],"award-info":[{"award-number":["104588"]}],"id":[{"id":"10.13039\/501100004621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CDDT"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:sec>\n<jats:title>Introduction:<\/jats:title>\n<jats:p>Candidiasis infection is caused by different species of Candida, which are\ncharacterized by host immunologic weakness. Black cumin seeds (Nigella sativa) have shown an inhibitory\neffect against Candida albicans. In this work, the inhibitory effect of standardized extract and\ndifferent fractions of Nigella sativa seeds has been evaluated on both nystatin-susceptible and resistant\nstrain of C. albicans.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Materials and Method:<\/jats:title>\n<jats:p>Canadida albicans (NSCA) with ATCC 76645 and nystatin-resistant Candida\nalbicans (NRCA) were prepared from oral samples of HIV individuals. Total extract and different\nfractions of N. sativa were prepared using maceration and sonication methods. Thymoquinone\n(TQ) content of the plant was determined by spectrophotometric method. Total extract (TTE) and the\nfractions along with TQ were evaluated on NSCA and NRCA by the microdilution method.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>TQ content of the plant was 0.92 \u00b1 0.37g\/100g dried extract. The least MIC and MFC (62.5\nand 125 \u03bcg\/ml, respectively) were due to petroleum ether fraction (PEF) against both NSCA and\nNRCA, followed by chloroform fraction (CHF) with MIC and MFC of 125 and 250 \u03bcg\/ml, respectively.\nTQ exhibited MIC of 0.78 and 3.12 \u03bcg\/ml against NSCA and NRCA, stronger than nystatin (MIC\nof 2 and 16 \u03bcg\/ml, respectively). Thymoquinone was detected in the PEF and CHF.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Considering more inhibitory effects of PEF and CHF than TTE, we can conclude that active\ncomponents of the plant belong to non-polar compounds. PEF showed identical inhibitory effects on\nNRCA and NSCA, which is a valuable result for finding novel medicaments against NRCA infections.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1570163819666220512164337","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T06:46:09Z","timestamp":1652424369000},"update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibitory Effect of Standardized Extract and Fractions of Nigella sativa\nL. on Nystatin Susceptible and Clinically Nystatin Resistant Candida albicans"],"prefix":"10.2174","volume":"19","author":[{"given":"Seyyed Amin","family":"Ayatollahi Mousavi","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Medical Mycology and Bacteriology Research Center, Kerman University of Medical\nSciences, Kerman, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fariba","family":"Sharififar","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Herbal and Traditional Medicines Research Center, Kerman University\nof Medical Sciences, Kerman, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abolfazl Saravani","family":"Jahantiq","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical Mycology and Bacteriology Research Center, Kerman University of Medical\nSciences, Kerman, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neda","family":"Mohamadi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Herbal and Traditional Medicines Research Center, Kerman University\nof Medical Sciences, Kerman, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","author":"Evans P.G.","year":"2019","unstructured":"Evans P.G.;  European whales, dolphins, and porpoises: marine mammal conservation in practice 2019","journal-title":"European whales, dolphins, and porpoises: marine mammal conservation in practice"},{"key":"ref=2","doi-asserted-by":"publisher","first-page":"2579","DOI":"10.2174\/1568026619666191025155856","volume":"19","author":"Kumar A.","year":"2019","unstructured":"Kumar A.; Khan F.; Saikia D.; Exploration of medicinal plants as sources of novel anticandidal drugs. 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