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Methionine aminopeptidase (MetAP) is a\npromising target for developing new antibiotics because it is essential for bacterial survival.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>To screen for potential MetAP inhibitors by in silico virtual screening of the ZINC database\nand evaluate the best potential lead molecules by in vitro studies.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>We have considered 200,000 compounds from the ZINC database for virtual screening with\nFlexX software to identify potential inhibitors against bacterial MetAP. Nine chemical compounds of\nthe top hits predicted were purchased and evaluated in vitro. The antimicrobial activity of each inhibitor\nof MetAP was tested by the disc-diffusion assay against one Gram-positive (Staphylococcus aureus)\nand two Gram-negative (Escherichia coli &amp; Pseudomonas aeruginosa) bacteria. Among the studied\ncompounds, compounds ZINC04785369 and ZINC03307916 showed promising antibacterial activity.\nTo further characterize their efficacy, the minimum inhibitory concentration was determined for\neach compound by the microdilution method which showed significant results.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>These results suggest compounds ZINC04785369 and ZINC03307916 as promising molecules\nfor developing MetAP inhibitors.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Furthermore, they could therefore serve as lead molecules for further chemical modifications\nto obtain clinically useful antibacterial agents.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1573409915666190617165643","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T09:35:48Z","timestamp":1561628148000},"page":"389-401","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Research of New Inhibitors of Bacterial Methionine Aminopeptidase by Structure Based Virtual Screening Approach of ZINC DATABASE and In Vitro Validation"],"prefix":"10.2174","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8042-8909","authenticated-orcid":true,"given":"Hanane","family":"Boucherit","sequence":"first","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences, Mentouri Brothers University, Constantine 1, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8850-8500","authenticated-orcid":true,"given":"Abdelouahab","family":"Chikhi","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences, Mentouri Brothers University, Constantine 1, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3467-6749","authenticated-orcid":true,"given":"Abderrahmane","family":"Bensegueni","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences, Mentouri Brothers University, Constantine 1, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2280-757X","authenticated-orcid":true,"given":"Amina","family":"Merzoug","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences, Mentouri Brothers University, Constantine 1, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3892-4340","authenticated-orcid":true,"given":"Jean-Michel","family":"Bolla","sequence":"additional","affiliation":[{"name":"Aix Marseille University, INSERM, SSA, MCT, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/j.idc.2016.02.004","volume":"30","author":"van Duin D.","year":"2016","unstructured":"van Duin D.; Paterson D.L.; Multidrug-resistant bacteria in the community: trends and lessons learned. 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