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This is a severe concern of this research, and an attempt\nwas made to prepare a target-specific formulation that could circumvent chemotherapy-related\ncompilation and improvise higher cellular uptake. The fundamental agenda of this research was\nto prepare and develop Anti-EGFR mAb and 5-Fluorouracil (5-FU) fabricated polymeric nanoparticles\nfor colorectal cancer.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>The main objective of this research was to prepare and evaluate more target specific formulation\nfor the treatment of colorectal cancer. PLGA and PEG-based polymeric nanoparticles are\ncapable of preventing opsonization via the reticuloendothelial system. Hence, prepared polymeric\nnanoparticles are capable of higher cellular uptake.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>The Poly(d,1-lactide-co-glycolide) (PLGA) and Polyethylene Glycol (PEG) were combined\nutilizing the ring-opening polymerization method. The presence of PEG prevents opsonization\nand distinguished blood concentration along with enhanced targeting. The presence of PLGA\nbenefits in the sustained release of polymeric formulations. The optimized formulation (5-FU-PLGA-\nPEG-NP) was lyophilized using 4% trehalose (cryoprotectants) and conjugated with Anti-\nEGFR mAb on its surface to produce Anti-EGFR-5-FU-PLGA-PEG-NP; the final formulation,\nwhich increases target specificity and drug delivery system of nanoparticles.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p> The spherical shaped optimized formulation, 5-FU-PLGA-PEG-NP-3 was found to have\nhigher percentage drug entrapment efficacy (71.23%), higher percentage drug content (1.98 \u00b1\n0.34%) with minimum particles size (252.3nm) and anionic zeta potential (-31.23mV). The IC<jats:sub>50<\/jats:sub>value\nof Anti-EGFR-5-FU-PLGA-PEG-NP was 1.01\u03bcg\/mL after 48 hours incubation period in the\nHCT 116 cell line, indicating higher anticancer effects of the final formulation.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions:<\/jats:title>\n<jats:p> From the outcomes of various experiments, it was concluded that Anti-EGFR-5-FU-PLGA-PEG-NP has\nbiphasic drug release kinetics, higher cellular uptake &amp; higher cytotoxicity. Therefore, Anti-EGFR-5-FU-PLGA-PEG-NP\nholds excellent potential for drug delivery to EGFR positive colorectal cancer cells.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1574892815666201221121859","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T04:29:36Z","timestamp":1608611376000},"page":"84-100","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Anti-EGFR-mAb and 5-Fluorouracil Conjugated Polymeric Nanoparticles for Colorectal Cancer"],"prefix":"10.2174","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0771-9582","authenticated-orcid":true,"given":"Sankha","family":"Bhattacharya","sequence":"first","affiliation":[{"name":"ISF College of Pharmacy, GT Road (NH-95), Ghal Kalan, Moga, Punjab 142001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","author":"Heydebreck CSV","year":"2012","unstructured":"Heydebreck CSV; Biomarkers and methods for determining efficacy of anti-EGFR antibodies in cancer therapy. 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