{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T18:44:06Z","timestamp":1783709046873,"version":"3.55.0"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"7","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["ACAMC"],"published-print":{"date-parts":[[2018,11,30]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p> Actinic Keratosis (AK), is the most common precancerous skin lesion induced by the\nexcessive Ultraviolet B (UVB) and is a significant threat to the public health. UVB exposure causes oxidative\nDNA damage and is considered to be a significant contributor to AK and subsequent development of skin cancer.\nBesides, activation of p38 MAPK also plays a significant role in the development of AK.\n<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p> This study aimed at the development of a nature compound which can inhibit UVB-induced AK.\n<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Method:<\/jats:title>\n<jats:p> MTS Cell Proliferation Assay Kit was used to detect the toxicity of astragalin. HE-staining, Immunohistochemical,\nWestern blot and Enzyme Linked Immunosorbent Assay were applied to examine the clinicopathologic\nfeature of AK and the change of p38 MAPK signal pathway treated with astraglin under the condition\nof UVB in vitro and in vivo.\n&lt;\/P&gt;&lt;P&gt;\nResults\uff1aIn our clinical findings revealed that p38 MAPK, phospho-MSK1, and \u03b3 -H2AX were significantly\nhighly expressed in human AK tissue than the normal healthy skin tissue. Moreover, in vitro studies showed that\nUVB induced the phospho-MSK1 and \u03b3-H2AX in a time- and dose-dependent manner in HaCaT cells. Further,\nin vitro kinase assay demonstrated that astragalin could directly bind to p38 MAPK and suppress p38 MAPK\nactivity. Furthermore, astragalin exhibited no toxicity and suppressed the UVB-induced expression of phospho-\nMSK1 and \u03b3 -H2AX by suppressing p38 MAPK activity in a time-dependent and dose-dependent manner in\nHaCaT cells. The in vivo studies with animal UV model demonstrated that astragalin inhibited UVB-induced\nexpression of phospho-MSK1 and \u03b3-H2AX in Babl\/c mice.\n<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>  These results suggested that p38 MAPK is a direct valid molecular target of astragalin for the attenuation\nof UVB-induced AK. Furthermore, astragalin could be a potential promising novel natural therapeutic\nagent for the prevention and management of UVB-induced AK with high target specificity and low toxicity.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871520618666171229190835","type":"journal-article","created":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T07:45:16Z","timestamp":1515051916000},"page":"1001-1008","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Astragalin Attenuates UVB Radiation-induced Actinic Keratosis Formation"],"prefix":"10.2174","volume":"18","author":[{"given":"Na","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Burns and Plastic Surgery, Shaanxi Nuclear Industry 215 Hospital, Xian yang 712000 Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenli","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaona","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Dematology, Affiliated Hospital of Yan'an University, Yan'an 716000 Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","container-title":["Anti-Cancer Agents in Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/158735","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/158735\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/158735","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T09:44:45Z","timestamp":1608975885000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/158735\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,30]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,11,30]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1871520618666171229190835","relation":{},"ISSN":["1871-5206"],"issn-type":[{"value":"1871-5206","type":"print"}],"subject":[],"published":{"date-parts":[[2018,11,30]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2017-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-27","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}