{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T13:55:52Z","timestamp":1722779752724},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"5","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["IDDT"],"published-print":{"date-parts":[[2020,12,9]]},"abstract":"<jats:sec>\n<jats:title>Introduction:<\/jats:title>\n<jats:p>In the context of the global threat of antimicrobial resistance (AMR)\namong bacterial pathogens against conventional bactericidal antibiotics, investigation on complementary\/\nalternative approaches to manage bacterial infections is warranted. The present study\naimed at investigating the anti-pathogenic potential of Phyllanthus emblica seed extract (PESE)\nagainst four different pathogenic bacteria.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> Hydroalcoholic extract of P. emblica seeds was tested for its possible in vitro quorummodulatory\npotential against Chromobacterium violaceum, Serratia marcescens, Pseudomonas\naeruginosa, and Staphylococcus aureus through broth dilution assay. In vivo efficacy of PESE\nwas assayed employing Caenorhabditis elegans as the model host for these four pathogens.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>PESE was found to exert in vitro quorum-modulatory effect on C. violaceum, S. marcescens,\nP. aeruginosa, and S. aureus at \u226550 \u03bcg\/mL. This extract could curb the haemolytic activity\nof all the four test bacteria by 23-65%, inhibit biofilm formation, and was also able to modulate\ntheir antibiotic susceptibility (AS) and catalase activity. Susceptibility of P. aeruginosa and S.\naureus to lysis by human serum was enhanced under the influence of this extract by 23% and\n49%, respectively. Repeated exposure of both these notorious pathogens to PESE did not induce\nresistance in them. In vivo assay confirmed the anti-virulence effect of this extract in the C. elegans\nhost, wherein the nematode host challenged with the PESE-treated pathogenic bacteria\nscored better survival. PESE also displayed notable prebiotic potential by promoting the growth of\nthree probiotic strains.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p> To the best of our awareness, this is the first report on the quorum-modulatory potential\nof P. emblica seed extract, validating its anti-infective potential and prebiotic property.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871526519666190821154926","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T16:29:33Z","timestamp":1566404973000},"page":"672-692","update-policy":"http:\/\/dx.doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Anti-infective Potential of Hydroalcoholic Extract of Phyllanthus emblica Seeds Against Selected Human-pathogenic Bacteria"],"prefix":"10.2174","volume":"20","author":[{"given":"Pooja","family":"Patel","sequence":"first","affiliation":[{"name":"Institute of Science, Nirma University, Ahmedabad- 382481, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinmayi","family":"Joshi","sequence":"additional","affiliation":[{"name":"Institute of Science, Nirma University, Ahmedabad- 382481, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay","family":"Kothari","sequence":"additional","affiliation":[{"name":"Institute of Science, Nirma University, Ahmedabad- 382481, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Infectious Disorders - Drug Targets"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/174385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T04:51:45Z","timestamp":1607575905000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/174385\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,9]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,12,9]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1871526519666190821154926","relation":{},"ISSN":["1871-5265"],"issn-type":[{"value":"1871-5265","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12,9]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-16","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}