{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:09:27Z","timestamp":1768810167845,"version":"3.49.0"},"reference-count":27,"publisher":"Bentham Science Publishers Ltd.","issue":"2","funder":[{"DOI":"10.13039\/501100005001","name":"Ahvaz Jundishapur University of Medical Sciences","doi-asserted-by":"publisher","award":["OG-93150"],"award-info":[{"award-number":["OG-93150"]}],"id":[{"id":"10.13039\/501100005001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["IDDT"],"published-print":{"date-parts":[[2021,5,20]]},"abstract":"<jats:sec>\n<jats:title>Introduction:<\/jats:title>\n<jats:p>The human being is considered a natural host for Trichomonas vaginalis\n(T. vaginalis), which causes trichomoniasis, the most frequent non-viral sexually transmitted infectious\ndisease in the world. The current study aimed to evaluate the prevalence and sequencing of T.\nvaginalis in women with vaginitis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p>In the current research, 514 vaginal discharge samples were obtained from women with\nvaginitis. The specimens were evaluated by the direct wet mount examination, Dorset culture medium,\nand PCR technique. Primers were designed for the detection of TVK3\/TVK7, TVA5\/TVA6\ngenes specific for the identification of T. vaginalis. The PCR-positive samples were sequenced and\ncompared with the sequences registered in the GenBank database.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Among the collected samples, 30 (5.83%), 45 (8.75%), 90 (17.50%), and 62 (12.06%) cases\nwere positive for T. vaginalis when assayed by the direct wet mount examination, Dorset culture\nmedium, and PCR technique (TVK3\/TVK7, TVA5\/TVA6 genes), respectively. There was no significant\nrelationship between trichomoniasis and demographic characteristics of women, such as age,\noccupational status, mode of delivery, number of deliveries, educational level, and contraceptive\nmethods (p\u02c30.05). The range of vaginal pH was between 5-7 in women with vaginitis, and there\nwas a significant statistical correlation between the pH values and the infection rate (p&lt;0.05). The\nPCR-positive samples had 100% sequence homology with the reference sequence in the GenBank\ndatabase (accession number L23861.1).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>This study confirmed a relatively high prevalence of T. vaginalis in the southwestern\nregion of Iran. According to our results, the PCR method, especially detecting TVK3\/TVK7 genes,\nwas more sensitive than the direct wet mount examination and Dorset culture medium methods.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871526520666200219110430","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T13:16:45Z","timestamp":1582118205000},"page":"262-267","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["PCR Detection and Sequencing of Trichomonas vaginalis in Women with Suspected Vaginitis in Southwestern Iran"],"prefix":"10.2174","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8997-9056","authenticated-orcid":true,"given":"Abdollah","family":"Rafiei","sequence":"first","affiliation":[{"name":"Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khatereh","family":"Safaie","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8337-6673","authenticated-orcid":true,"given":"Mehdi","family":"Tavalla","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6649-3931","authenticated-orcid":true,"given":"Mahin","family":"Najafian","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.1007\/s00436-015-4393-3","volume":"114","author":"Hezarjaribi H.Z.","year":"2015","unstructured":"Hezarjaribi H.Z.; Fakhar M.; Shokri A.; Teshnizi S.H.; Sadough A.; Taghavi M.; Trichomonas vaginalis infection among Iranian general population of women: a systematic review and meta-analysis. 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