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Many herbal extracts and their derivatives have been used\nagainst trophozoites and cysts of Acanthamoeba, but no effective therapeutic agent has yet been discovered.\nTherefore, the present study aimed to evaluate the effect of Rumex obtusifolius (R. obtusifolius)\nextracts against a clinical strain of Acanthamoeba genotype T4 in vitro.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> In this experimental study, after genotyping the clinical isolate, the hydroalcohlic\nextracts of R. obtusifolius seeds and leaves were prepared. Different concentrations (1.25, 2.5,\n5 and 10 mg\/ml) of extracts were tested in triplicate (24, 48 and 72h) on trophozoites and\ncysts of Acanthamoeba. Mortality of the parasite was assessed by trypan blue vital staining\nand flow cytometry analysis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Results showed that the extract of R. obtusifolius leaves at the concentration of 10 mg\/ml\nkilled 100% of trophozoites and cysts after 72 h. However, the seed extract of R. obtusifolius had\nweak inhibitory effects on trophozoites and cysts of Acanthamoeba. In the presence of 10 mg\/ml of\nhydroalcoholic seed extract of R.obtusifolius in culture medium after 72 h, 28.6% of trophozoites\nand 0% of cysts of Acanthamoeba were killed. After analysis by flow cytometry, seeds and leaves\nextract indicated apoptosis effect. Seed and leaf extracts caused 2.6% and 0.4% percent apoptosis.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>These extracts are not promising candidates for further medicine development\non acanthamoebiasis. Nonetheless, further researches are necessary to clarify effective\nfractions of seed and leaf extracts of R. obtusifolius and their mechanisms of action.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871526520666200422111044","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T03:12:48Z","timestamp":1587611568000},"page":"211-219","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the Effects of Rumex obtusifolius Seed and Leaf Extracts Against Acanthamoeba: An in vitro Study"],"prefix":"10.2174","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5766-0120","authenticated-orcid":true,"given":"Tooran","family":"Nayeri","sequence":"first","affiliation":[{"name":"Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5472-7470","authenticated-orcid":true,"given":"Farahnaz","family":"Bineshian","sequence":"additional","affiliation":[{"name":"Department of Parasitology & Mycology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2738-0552","authenticated-orcid":true,"given":"Fariba","family":"Khoshzaban","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Shahed University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5591-5513","authenticated-orcid":true,"given":"Abdolhossein Dalimi","family":"Asl","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-8214","authenticated-orcid":true,"given":"Fatemeh","family":"Ghaffarifar","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.exppara.2017.09.011","volume":"183","author":"Hajaji S.","year":"2017","unstructured":"Hajaji S.; Sifaoui I.; L\u00f3pez-Arencibia A.; Reyes-Batlle M.; Jim\u00e9nez I.A.; Bazzocchi I.L.; Valladares B.; Pinero J.E.; Lorenzo-Morales J.; Akkari H.; Correlation of radical-scavenging capacity and amoebicidal activity of Matricaria recutita L. 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