{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:45:44Z","timestamp":1787237144207,"version":"build-2736575974"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"5","funder":[{"DOI":"10.13039\/501100004621","name":"Kerman University of Medical Sciences","doi-asserted-by":"publisher","award":["95000291"],"award-info":[{"award-number":["95000291"]}],"id":[{"id":"10.13039\/501100004621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["IDDT"],"published-print":{"date-parts":[[2021,8]]},"abstract":"\n Background:<\/jats:title>\n The dog tapeworm, Echinococcus granulosus, is a zoonotic parasite affecting\nhumans and livestock across the globe. Basic research on the molecular biology and genetics of\nE. granulosus improves our understanding of the biology and potential drug targets in various developmental\nstages of E. granulosus in both definitive and intermediate hosts. There has been increasing\ninterest in the identification of microRNAs in parasitic organisms. The purpose of the current\nstudy was to compare the activity of a selected profile of miRNAs in different developmental\nstages of E. granulosus.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Different developmental stages of the parasite were obtained from ex vivo as well as in\nvitro cultured E. granulosus. MicroRNAs were extracted from the ex vivo germinal layer and invaginated\nprotoscoleces as well as the in vitro generated microcysts, evaginated protoscoleces, and\nstrobilated worms. The expression of the selected miRNAs was evaluated by RT-qPCR for each\nstage.<\/jats:p>\n <\/jats:sec>\n \n Results::<\/jats:title>\n Four out of five miRNAs were present and active in different developmental stages of E.\ngranulosus. A significant over-expression of miR-61 was observed in the germinal layer and during\nthe protoscolex transformation into the microcysts, however, miR-10 was more expressed in\nthe mature strobilated forms than in the other stages. Let-7 and miR-3489 showed a high expression\nin the germinal layer.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Differential expression of four miRNAs among different in vitro and ex vivo developmental\nstages of E. granulosus was documented in the present study. Further experimental investigations\nare required to elucidate the probable role of the miRNAs in bi-directional differentiation\nof protoscoleces either into the strobilated worm or to a secondary hydatid cyst and the potential of\nthese miRNAs as drug targets.<\/jats:p>\n <\/jats:sec>","DOI":"10.2174\/1871526520999201103192518","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T09:45:21Z","timestamp":1604655921000},"update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative Analysis of miRNA Expressions in Different Developmental Stages of Echinococcus granulosus in Mono-Phasic and Di-Phasic Culture Systems"],"prefix":"10.2174","volume":"21","author":[{"given":"Seifollah","family":"Mortezaei","sequence":"first","affiliation":[{"name":"Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7616914115, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Afgar","sequence":"additional","affiliation":[{"name":"Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7616914115, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balal","family":"Sadeghi","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ali","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7616914115, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7616914115, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3257-5389","authenticated-orcid":true,"given":"Majid Fasihi","family":"Harandi","sequence":"additional","affiliation":[{"name":"Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7616914115, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","container-title":["Infectious Disorders - Drug Targets"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/187569","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/187569\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/187569","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T12:26:41Z","timestamp":1632140801000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/187569\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1871526520999201103192518","relation":{},"ISSN":["1871-5265"],"issn-type":[{"value":"1871-5265","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2020-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-21","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e270421187569"}}