{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T07:22:40Z","timestamp":1747207360820,"version":"3.40.5"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"10","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["EMIDDT"],"published-print":{"date-parts":[[2020,12,2]]},"abstract":"\nObjective:<\/jats:title>\nPolyphenols extracted by table grape have been demonstrated to decrease cell\nproliferation in vitro and to exert anti-atherosclerotic and antithrombotic activities, regulating cell functions.\nA grape polyphenolic profile is affected by climate as well as a grape cultivar. This study was\naimed to characterize the berry skin polyphenolic composition, antioxidant activity and antiproliferative\nproperties of two black grape cultivars, Autumn Royal and Egnatia.<\/jats:p>\n<\/jats:sec>\n\nMethods:<\/jats:title>\nThe phenolic composition of Grape Skin Extracts (GSEs) was determined by HPLC analyses.\nThe antioxidant activity was determined using DPPH, ABTS and ORAC tests. Caco2, HT29 and\nSW480 human colon cancer cell lines were used to test the effects of GSEs in vitro. Cell proliferation\nand cell cycle were assessed with the MTT method and a Muse cell analyzer, respectively. qPCR and\nWestern Blotting analysis were used to evaluate gene and protein expression, respectively.<\/jats:p>\n<\/jats:sec>\n\nResults:<\/jats:title>\nThe total polyphenolic content and the total antioxidant capacity were significantly higher in\nAutumn Royal than in Egnatia. However, table grape Egnatia showed greater ability to affect cell proliferation\nand apoptosis, as well as to exert a growth arrest in the S phase of the cell cycle, particularly\nin the Caco2 cell line.<\/jats:p>\n<\/jats:sec>\n\nConclusion:<\/jats:title>\nThese data suggest that the new grape variety Egnatia is an interesting source of phenolic\ncompounds that could be of interest in the food and pharmaceutical industries.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871530320666200421102418","type":"journal-article","created":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T02:20:41Z","timestamp":1587522041000},"page":"1740-1750","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Autumn Royal and Egnatia Grape Extracts Differently Modulate Cell Proliferation in Human Colorectal Cancer Cells"],"prefix":"10.2174","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3712-4149","authenticated-orcid":true,"given":"Isabella","family":"Gigante","sequence":"first","affiliation":[{"name":"Laboratory of Nutritional Biochemistry, National Institute of Gastroenterology \u201cS. de Bellis\u201d Research Hospital, 70013 Castellana Grotte (Ba), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3897-7477","authenticated-orcid":true,"given":"Rosa Anna","family":"Milella","sequence":"additional","affiliation":[{"name":"Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics, 70010 Turi, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Tutino","sequence":"additional","affiliation":[{"name":"Laboratory of Nutritional Biochemistry, National Institute of Gastroenterology \u201cS. de Bellis\u201d Research Hospital, 70013 Castellana Grotte (Ba), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-2530","authenticated-orcid":true,"given":"Giambattista","family":"Debiase","sequence":"additional","affiliation":[{"name":"Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics, 70010 Turi, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6120-3651","authenticated-orcid":true,"given":"Luciano","family":"Notarangelo","sequence":"additional","affiliation":[{"name":"Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics, 70010 Turi, Bari"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9142-0956","authenticated-orcid":true,"given":"Maria Angela","family":"Giannandrea","sequence":"additional","affiliation":[{"name":"Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics, 70010 Turi, Bari"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"De Nunzio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8255-4776","authenticated-orcid":true,"given":"Antonella","family":"Orlando","sequence":"additional","affiliation":[{"name":"Laboratory of Nutritional Pathophysiology, National Institute of Gastroenterology \u201cS. de Bellis\u201d Research Hospital, 70013 Castellana Grotte (Ba), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosalba","family":"D\u2019Alessandro","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Biology, National Institute of Gastroenterology, \"S. de Bellis\" Research Hospital, 70013 Castellana Grotte, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4518-6307","authenticated-orcid":true,"given":"Maria Gabriella","family":"Caruso","sequence":"additional","affiliation":[{"name":"Ambulatory of Clinical Nutrition, National Institute of Gastroenterology \u201cS. de Bellis\u201d Research Hospital, 70013 Castellana Grotte (Ba), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2274-2198","authenticated-orcid":true,"given":"Maria","family":"Notarnicola","sequence":"additional","affiliation":[{"name":"Laboratory of Nutritional Biochemistry, National Institute of Gastroenterology \u201cS. de Bellis\u201d Research Hospital, 70013 Castellana Grotte (Ba), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Endocrine, Metabolic & Immune Disorders - Drug Targets"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/181126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T05:09:45Z","timestamp":1606972185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/181126\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,2]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,12,2]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1871530320666200421102418","relation":{},"ISSN":["1871-5303"],"issn-type":[{"type":"print","value":"1871-5303"}],"subject":[],"published":{"date-parts":[[2020,12,2]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-03","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}