{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:07:38Z","timestamp":1778814458800,"version":"3.51.4"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"11","funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Science","doi-asserted-by":"publisher","award":["94-7599"],"award-info":[{"award-number":["94-7599"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["EMIDDT"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6\u20137% of\npremenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the\ncontext PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these\ncells in the context of PCOS.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p> To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using\ntwo-dimensional gel electrophoresis (2DE) and mass spectrometry (MS).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> In the present study, we applied two-dimensional gel electrophoresis \/ mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western\nblot technique was used to confirm the identified proteins.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P &lt;0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator\ncomplex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P &lt;0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular\nmetabolism especially glycolysis and ferroptosis pathway.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871530320666201119152323","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T04:19:17Z","timestamp":1605845957000},"page":"1998-2004","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients"],"prefix":"10.2174","volume":"21","author":[{"given":"Fatemeh","family":"Nasri","sequence":"first","affiliation":[{"name":"Department of Immunology, Shiraz University of Medical Sciences, Shiraz,Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Zare","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shiraz University of Medical Sciences, Shiraz,Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehrnoosh","family":"Doroudchi","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shiraz University of Medical Sciences, Shiraz,Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Behrouz","family":"Gharesi-Fard","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shiraz University of Medical Sciences, Shiraz,Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Endocrine, Metabolic &amp; Immune Disorders - Drug Targets"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/188201","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.eurekaselect.com\/188201\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/eurekaselect.com\/article\/download\/188201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T19:02:56Z","timestamp":1641322976000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/188201\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1871530320666201119152323","relation":{},"ISSN":["1871-5303"],"issn-type":[{"value":"1871-5303","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2020-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-25","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}