{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:45:14Z","timestamp":1791960314693,"version":"4.3.4"},"reference-count":47,"publisher":"Bentham Science Publishers Ltd.","issue":"4","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["BIOT"],"published-print":{"date-parts":[[2019,10,25]]},"abstract":"<jats:sec>\n                    <jats:title>Background &amp; Objectives:<\/jats:title>\n                    <jats:p>In our previous investigation, oral administration of 1,8- cineole-rich supercritical carbon dioxide extract of small cardamom seeds in Wistar albino rats resulted in achieving normal fasting blood glucose (FBG) and serum cholesterol levels. The objective of this study was to further protect the aforesaid extract and to enhance its in vivo therapeutic efficacies in redressing type 2 diabetes and hypercholesterolemia, by encapsulating it as nanoliposomes. Patents related to nanoliposomes have been revised thoroughly.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>PEGylated nanoliposomes of the aforesaid extract were formulated using soya phosphatidylcholine and Tween 80 by probe-sonication. These nanoliposomes were subjected to in vitro characterizations and were orally administered to Wistar albino rats at three different doses viz. 550, 175 and 55 mg\/kg b.w. for detailed investigation of their antidiabetic and hypocholesterolemic efficacies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>FT-IR, DSC and XRD analyses, HLB value (16), entrapment efficiency (84%) and release kinetics (obeying Higuchi model) revealed that the nanoliposomes were o\/w type and were hydrophilic. They exhibited appreciable in vitro antioxidant potency (59% DPPH scavenging activity) owing to a synergistic consortium of antioxidants present therein. Oral administration of the liposomes in rats at 550 mg\/kg b.w. could restore their normal FBG levels and serum lipid profiles on day 35, with desirable up-down regulations of related key enzymes. The iHOMA2 model could successfully predict the effects of nanoliposomes on insulin sensitivity and glucose uptake in rat liver and brain, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Nanoliposome of 1,8-cineole rich extract of small cardamom seeds is a new biotherapeutic in redressing type 2 diabetes and hypercholesterolemia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2174\/1872208313666190404101336","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T05:41:52Z","timestamp":1554788512000},"page":"284-303","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Nanoliposomes of Supercritical Carbon Dioxide Extract of Small Cardamom Seeds Redresses Type 2 Diabetes and Hypercholesterolemia"],"prefix":"10.2174","volume":"13","author":[{"given":"Kaninika","family":"Paul","sequence":"first","affiliation":[{"name":"Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700 032, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paramita","family":"Bhattacharjee","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700 032, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilendra","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"TAAB Biostudy Services, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tapan Kumar","family":"Pal","sequence":"additional","affiliation":[{"name":"Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"ref=1","first-page":"370","author":"Farooqi A.A.","year":"2005","unstructured":"Farooqi A.A.; Sreeramu B.S.; Srinivasappa K.N.; In Small cardamom 2005,370","journal-title":"In Small cardamom"},{"key":"ref=2","first-page":"230","volume":"27","author":"Korikontimath V.S.","year":"1999","unstructured":"Korikontimath V.S.; Mulge R.Z.; Achariah J.T.; Variations in essential oil constituents in high yielding selections of cardamom. 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J Med Chem 2016,59,1671-1690","journal-title":"J Med Chem"},{"key":"ref=6","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1080\/00986445.2018.1428959","volume":"205","author":"Paul K.","year":"2018","unstructured":"Paul K.; Bhattacharjee P.; Mass transfer and hydrodynamic study of supercritical carbon dioxide extraction of 1,8-cineole from small cardamom seeds. Chem Engg Comm 2018,205,1023-1033","journal-title":"Chem Engg Comm"},{"key":"ref=7","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.tifs.2017.07.017","volume":"68","author":"Katouzian I.","year":"2017","unstructured":"Katouzian I.; Esfanjani A.F.; Jafari S.M.; Akhavan S.; Formulation and application of a new generation of lipid nano-carriers for the food bioactive ingredients. 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US7951396B2 2011"},{"key":"ref=11","author":"Venkatraman S","year":"2017","unstructured":"Venkatraman S; Natarajan JV; Boey YCF; Mehta JS; Howden TTL; NG XW. Nanoliposomes for sustained delivery of tacrolimus for treatment of anterior segment eye diseases. WO2017\/184080Al 2017"},{"key":"ref=12","author":"Zhang Y.","year":"2012","unstructured":"Zhang Y.; Method of making liposomes, liposome compositions made by the methods, and methods of using the same. US2012\/0171280A1 2012"},{"key":"ref=13","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2174\/138920012798356934","volume":"13","author":"Milla P.","year":"2012","unstructured":"Milla P.; Dosio F.; Cattel L.; PEGylation of proteins and liposomes: A powerful and flexible strategy to improve the drug delivery. 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Diabetes Care 2013,36(8),2324-2330","journal-title":"Diabetes Care"},{"key":"ref=15","first-page":"243","author":"Ranganna S.","year":"1986","unstructured":"Ranganna S.; Handbook of analysis and quality control for fruits and vegetable products New Delhi 1986,243-285","journal-title":"Handbook of analysis and quality control for fruits and vegetable products New Delhi"},{"key":"ref=16","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1080\/01496395.2014.973517","volume":"50","author":"Ghosh S.","year":"2015","unstructured":"Ghosh S.; Bhattacharjee P.; Das S.; 1,8-cineol-rich cardamom seed (Elettaria cardamomum) extracts using green technologies and conventional extractions: Process analysis, phytochemical characterization and food application. Sep Sci Technol 2015,50(13),1974-1985","journal-title":"Sep Sci Technol"},{"key":"ref=17","first-page":"42","volume":"7","author":"Kuyumcu E.","year":"2013","unstructured":"Kuyumcu E.; K\u00fc\u00e7\u00fckba F.Z.; Essential oil composition of Elettaria cardamomum Maton. J Appl Biol Sci 2013,7(3),42-45","journal-title":"J Appl Biol Sci"},{"key":"ref=18","author":"Stein S.E.","year":"2007","unstructured":"Stein S.E.; Mass Spectra. Mallard Gaithersburg NIST Chemistry Web Book. 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BMC Complem Altern M 2010,10(21),2-8","journal-title":"BMC Complem Altern M"},{"key":"ref=21","first-page":"2486","volume":"8","author":"Chakraborty S.","year":"2017","unstructured":"Chakraborty S.; Bhattacharjee P.; Supercritical carbon dioxide extraction of melatonin from Brassica campestris: In vitro antioxidant, hypocholesterolemic and hypoglycaemic activities of the extracts. Int J Pharm Sci Res 2017,8(6),2486-2495","journal-title":"Int J Pharm Sci Res"},{"key":"ref=22","unstructured":"OECD (Organisation for Economic Co-operation and Development) 423, 2001. Organization of economic co-operation and development guidelines. [Available at:[Accessed on: May, 2017]."},{"key":"ref=23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.colsurfa.2011.01.027","volume":"377","author":"Lesoin L.","year":"2011","unstructured":"Lesoin L.; Boutin O.; Crampon C.; Badens E.; CO\/water\/surfactant ternary systems and liposome formation using supercritical CO: a review. Colloids Surf A Physicochem Eng Asp 2011,377(1-3),1-14","journal-title":"Colloids Surf A Physicochem Eng Asp"},{"key":"ref=24","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jfoodeng.2017.01.006","volume":"201","author":"Dutta S.","year":"2017","unstructured":"Dutta S.; Bhattacharjee P.; Nanoliposomal encapsulates of piperine-rich black pepper extract obtained by enzyme-assisted supercritical carbon dioxide extraction. 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J Lipid Res 1983,24(2),120-130","journal-title":"J Lipid Res"},{"key":"ref=28","first-page":"219","author":"Lewis M.S.","year":"2006","unstructured":"Lewis M.S.; Ullrey D.E.; Barnard D.E.; Knapka J.J.; In Suckow MA, Weisbroth SH, Franklin CL, Eds Nutrition 2006,219-301","journal-title":"In Suckow MA, Weisbroth SH, Franklin CL, Eds Nutrition"},{"key":"ref=29","first-page":"1","volume":"2013","author":"Cheng D.","year":"2013","unstructured":"Cheng D.; Liang B.; Li Y.; Antihyperglycemic effect of ginkgo biloba extract in streptozotocin-induced diabetes in rats. BioMed Res Int 2013,2013,1-7","journal-title":"BioMed Res Int"},{"key":"ref=30","unstructured":"OECD (Organisation for Economic Co-operation and Development) 425, 2001. Organization of economic co-operation and development guidelines. Available at:[Accessed on: June, 2017]."},{"key":"ref=31","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1042\/bj2920267","volume":"292","author":"Shibib B.A.","year":"1993","unstructured":"Shibib B.A.; Khan L.A.; Rahman R.; Hypoglycaemic activity of Coccinia indica and Momordica charantia in diabetic rats: Depression of the hepatic gluconeogenic enzymes glucose-6-phosphatase and fructose-1, 6-bisphosphatase and elevation of both liver and red-cell shunt enzyme glucose-6-phosphate dehydrogenase. Biochem J 1993,292(1),267-270","journal-title":"Biochem J"},{"key":"ref=32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cbi.2014.07.007","volume":"221","author":"Ahmad A.","year":"2014","unstructured":"Ahmad A.; Ahmad R.; Resveratrol mitigate structural changes and hepatic stellate cell activation in N\u2032-Nitrosodimethylamine-induced liver fibrosis via restraining oxidative damage. 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Int J Recent Sci Res 2014,5,1192-1197","journal-title":"Int J Recent Sci Res"},{"key":"ref=36","first-page":"680","volume":"7","author":"Namani S.","year":"2016","unstructured":"Namani S.; Gogineni R.; Bandi A.; Gandham R.; Brahmanapally V.; Evaluation of Non-HDL cholesterol and total cholesterol\/HDL-C ratio, LDL-C\/HDL-C ratio as cumulative marker of cardiovascular disease risk in type 2 diabetes mellitus patients. Int J Biomed Res 2016,7,680-684","journal-title":"Int J Biomed Res"},{"key":"ref=37","doi-asserted-by":"crossref","first-page":"370","DOI":"10.22376\/ijpbs.2016.7.4.b20-32","volume":"7","author":"Mallick S.","year":"2016","unstructured":"Mallick S.; Mandal M.; Roy S.; Effect of phytosterol extract from sesame seed on experimentally Induced hyperlipidemic rats: dose dependent study. Int J Pharma Bio Sci 2016,7,370-377","journal-title":"Int J Pharma Bio Sci"},{"key":"ref=38","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1093\/clinchem\/21.10.1523","volume":"21","author":"Rao A.V.","year":"1975","unstructured":"Rao A.V.; Ramakrishnan S.; Indirect assessment of hydroxymethylglutaryl-CoA reductase (NADPH) activity in liver tissue. Clin Chem 1975,21(10),1523-1525","journal-title":"Clin Chem"},{"key":"ref=39","unstructured":"Bio-Rad Laboratories. SpectraBase. Available at:[Accessed on: June 06, 2018]."},{"key":"ref=40","unstructured":"Bio-Rad Laboratories. SpectraBase. Available at:(Accessed on: June 06, 2018)."},{"key":"ref=41","doi-asserted-by":"crossref","first-page":"30","DOI":"10.11648\/j.ajop.20170503.12","volume":"5","author":"Boughendjioua H.","year":"2017","unstructured":"Boughendjioua H.; Djeddi S.; Fourier transformed infrared spectroscopy analysis of constituents of lemon essential oils from Algeria. Am J Optics Photon 2017,5(3),30-35","journal-title":"Am J Optics Photon"},{"key":"ref=42","unstructured":"Bio-Rad Laboratories. SpectraBase. [Available at:[Accessed on: June 06, 2018]."},{"key":"ref=43","first-page":"143","author":"Dyer J.R.","year":"2012","unstructured":"Dyer J.R.; Ed Infrared spectroscopy 2012,143-60","journal-title":"Infrared spectroscopy"},{"key":"ref=44","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0378-5173(94)00314-U","volume":"117","author":"Memoli A.","year":"1995","unstructured":"Memoli A.; Palermiti L.G.; Travagli V.; Alhaique F.; Egg and soya phospholipids-sonication and dialysis: a study on liposome characterization. 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