{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T06:52:30Z","timestamp":1765608750659},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"4","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["BIOT"],"published-print":{"date-parts":[[2020,11,27]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Snakebite envenomation is a global priority ranked top among\nother neglected tropical diseases. There is a folkloric claim that Uvaria chamae is beneficial\nfor the management of snakebite and wounds in African ethnobotanical surveys.\nBesides, there are many registered patents asserting the health benefits of U. chamae.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p> This study aimed to investigate U. chamae\u2019s potentials and identify candidates\nfor the development of tools for the treatment and management of N. nigricollis envenomation.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> Freshly collected U. chamae leaves were air-dried, powdered, and extracted in\nmethanol. The median lethal dose of the extract was determined and further fractionated\nwith n-hexane, n-butanol and ethyl acetate. Each fraction was tested for neutralizing effect\nagainst venom-induced haemolytic, fibrinolytic, hemorrhagic, and cytotoxic activities.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>U. chamae fractions significantly (p&lt;0.05) neutralized the haemolytic activity of\nN. nigricollis venom in n-butanol; 31.40%, n-hexane; 33%, aqueous residue; 39.60% and\nethyl acetate; 40.70% at the concentration of 100mg\/ml of each fraction against 10mg\/ml\nof the snake venom when compared to the positive control. The fibrinolytic activity of\nN. nigricollis venom was significantly (p&lt;0.05) neutralized in n-hexane at 73.88%,\nn-butanol; 72.22% and aqueous residue; 72.22% by the fractions of U. chamae. In\naddition, haemorrhagic activity of N. nigricollis venom was significantly (p&lt;0.05) neutralized\nby U. chamae fractions at the concentrations of 100mg\/ml, 200mg\/ml and 400mg\/ml\nexcept for n-butanol and aqueous residues at 400 mg\/ml.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p> U. chamae leaves fractions possess a high level of protection against\nN. nigricollis venoms-induced lethality and thus validate the pharmacological rationale for\nits usage in the management of N. nigricollis envenomation.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1872208314666200903152129","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T11:38:55Z","timestamp":1599219535000},"page":"295-311","update-policy":"http:\/\/dx.doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["In Vitro and In Vivo Neutralizing Activity of Uvaria chamae Leaves Fractions on the Venom of Naja nigricollis in Albino Rat and Bovine Blood"],"prefix":"10.2174","volume":"14","author":[{"given":"Ada","family":"Gabriel","sequence":"first","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamman","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed G.","family":"Magaji","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuf P.","family":"Ofemile","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ameh P.","family":"Matthew","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isaac O.","family":"Akefe","sequence":"additional","affiliation":[{"name":"Queensland Brain Institute, The University of Queensland, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","container-title":["Recent Patents on Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/185544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T07:43:39Z","timestamp":1606722219000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/185544\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,27]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,11,27]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1872208314666200903152129","relation":{},"ISSN":["1872-2083"],"issn-type":[{"value":"1872-2083","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11,27]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2020-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-10","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}