{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:51:21Z","timestamp":1760550681949},"reference-count":33,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T00:00:00Z","timestamp":1539820800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["openbiomedicalengineeringjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TOBEJ"],"published-print":{"date-parts":[[2018,10,18]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Cultured bovine-periosteum-derived cells can form three-dimensional structures on tissue culture dishes without artificial scaffolding material, can induce bone regeneration <jats:italic>in vivo<\/jats:italic>. The utility of cultured bovine-periosteum-derived cells for bone tissue regeneration after their transplantation into nude mice has been reported, the precise F-box molecular mechanism was unclear.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>The aim of this study was to investigate the specific F-box proteins required for bone regeneration by cultured bovine-periosteum-derived cells <jats:italic>in vitro<\/jats:italic>.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In the present study, periosteum tissue and cultured periosteum-derived cells were cultured for 5 weeks <jats:italic>in vitro<\/jats:italic> and then embedded in collagen gel with a green tissue-marking dye. Electrophoresis and immunohistochemistry were used to identify the specific F-box proteins required for tissue bone regeneration.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The bovine-periosteum-derived cells were observed to form bone shortly after the expression of F-box proteins. After the initial phase of bone formation, the expression of the F-box proteins ceased. FBXW2 was shown to be expressed in the periosteum, but not in cultured periosteum-derived cells. Furthermore, FBXL14 disappeared during bone formation.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Bone regeneration requires progenitor cells, such as bovine-periosteum-derived cells and the activation of the F-box Proteins FBXW2 and FBXL14, over time the expression of these proteins ceases. Further scientific and clinical trials are needed to investigate how the F-box Proteins can be used therapeutically to treat osteoporosis and osteonecrosis.<\/jats:p>\n                <\/jats:sec>","DOI":"10.2174\/1874120701812010075","type":"journal-article","created":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T03:17:28Z","timestamp":1540178248000},"page":"75-89","update-policy":"http:\/\/dx.doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of the F-box Proteins FBXW2 and FBXL14 in the Initiation of Bone Regeneration in Transplants given to Nude Mice"],"prefix":"10.2174","volume":"12","author":[{"given":"Mari","family":"Akiyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.bone.2010.10.178","volume":"48","author":"Wang Q.","year":"2011","unstructured":"Wang Q, Huang C, Xue M, Zhang X. \n                    Expression of endogenous BMP-2 in periosteal progenitor cells is essential for bone healing. \n                    Bone  \n                    2011; \n                    48\n                    (3)\n                    : 524-32.","journal-title":"Bone"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.bone.2014.08.007","volume":"70","author":"Roberts S.J.","year":"2015","unstructured":"Roberts SJ, van Gastel N, Carmeliet G, Luyten FP. \n                    Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath. \n                    Bone  \n                    2015; \n                    70\n                    : 10-8.","journal-title":"Bone"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1007\/s00256-009-0849-9","volume":"39","author":"Dwek J.R.","year":"2010","unstructured":"Dwek JR. \n                    The periosteum: what is it, where is it, and what mimics it in its absence? \n                    Skeletal Radiol  \n                    2010; \n                    39\n                    (4)\n                    : 319-23.","journal-title":"Skeletal Radiol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"S45","DOI":"10.1089\/10763270360696978","volume":"9","author":"Hutmacher D.W.","year":"2003","unstructured":"Hutmacher DW, Sittinger M. \n                    Periosteal cells in bone tissue engineering. \n                    Tissue Eng  \n                    2003; \n                    9\n                    (Suppl. 1)\n                    : S45-64.","journal-title":"Tissue Eng."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"881","DOI":"10.7150\/ijms.13172","volume":"12","author":"Sung I.Y.","year":"2015","unstructured":"Sung IY, Park BC, Hah YS, et al. \n                    FOXO1 is involved in the effects of cigarette smoke extract on osteoblastic differentiation of cultured human periosteum-derived cells. \n                    Int J Med Sci  \n                    2015; \n                    12\n                    (11)\n                    : 881-90.","journal-title":"Int. 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