{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T06:52:30Z","timestamp":1761547950996,"version":"build-2065373602"},"reference-count":14,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2012,10,5]],"date-time":"2012-10-05T00:00:00Z","timestamp":1349395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["openneurologyjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TONEUJ"],"published-print":{"date-parts":[[2012,10,5]]},"abstract":"<jats:p>\n                    Lyme disease\n                    <jats:italic>Borrelia<\/jats:italic>\n                    are invasive, nontoxigenic, persistent pathogens, and little is known about their mechanisms of pathogenesis. In our laboratory, a signature-tagged mutagenesis (STM) library of over 4,000\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    transposon mutants has been constructed and is being screened for infectivity in mice. In this manner, a global view of the virulence determinants (factors required for full infectivity) is being developed. Additionally, the mechanisms of immune evasion involving the VMP-like system (\n                    <jats:italic>vls<\/jats:italic>\n                    ) are under analysis, and cryo-electron microscopy is providing a detailed view of the three-dimensional structure of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    . These approaches will contribute to the improved understanding of how Lyme disease\n                    <jats:italic>Borrelia<\/jats:italic>\n                    cause disease.\n                  <\/jats:p>","DOI":"10.2174\/1874205x01206010119","type":"journal-article","created":{"date-parts":[[2012,10,12]],"date-time":"2012-10-12T02:11:31Z","timestamp":1350007891000},"page":"119-123","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["How do Lyme Borrelia Organisms Cause Disease? The Quest for Virulence Determinants#"],"prefix":"10.2174","volume":"06","author":[{"given":"Steven J","family":"Norris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"refR1","doi-asserted-by":"crossref","first-page":"6947","DOI":"10.1073\/pnas.97.13.6947","volume":"97","author":"Schopf JW","year":"2000","unstructured":"Schopf JW. \n            Solution to Darwin's dilemma: Discovery of the missing Precambrian record of life \n            Proc Natl Acad Sci USA  \n            2000; \n            97\n            (13\n            )\n            : 6947-53.","journal-title":"Proc Natl Acad Sci USA"},{"key":"refR2","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1046\/j.1365-2958.1999.01654.x","volume":"34","author":"Coburn J","year":"1999","unstructured":"Coburn J, Chege W, Magoun L, Bodary SC, Leong JM. \n            Characterization of a candidate Borrelia burgdorferi beta3-chain integrin ligand identified using a phage display library \n            Mol Microbiol  \n            1999; 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