{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T06:49:20Z","timestamp":1761547760723,"version":"build-2065373602"},"reference-count":17,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T00:00:00Z","timestamp":1213315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["openmicrobiologyjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TOMICROJ"],"published-print":{"date-parts":[[2008,6,13]]},"abstract":"<jats:p>\n                    Consecutively collected synovial fluids were examined for presence of bacterial DNA (a 700-bp fragment of the bacterial 23S rRNA gene) followed by DNA sequencing of amplicons, and by conventional bacteriological methods. One or more microorganisms were identified in 22 of the 227 synovial fluids (9,7%) originating from 17 patients. Sixteen of the patients had clinical signs of arthritis. For 11 patients molecular and conventional bacterial examinations were in agreement.\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus dysgalactiae subspecies equisimilis<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    , were detected in synovial fluids from 6, 2 and 2 patients, respectively. In 3 patients only 23S rRNA analysis was positive; 2 synovial fluids contained\n                    <jats:italic>S. dysgalactiae subspecies equisimilis<\/jats:italic>\n                    and 1\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    ). The present study indicates a significant contribution by PCR with subsequent DNA sequencing of the 23S rRNA gene analysis in recognizing and identification of microorganisms from synovial fluids.\n                  <\/jats:p>","DOI":"10.2174\/1874285800802010085","type":"journal-article","created":{"date-parts":[[2008,8,4]],"date-time":"2008-08-04T12:01:26Z","timestamp":1217851286000},"page":"85-88","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Infective Arthritis: Bacterial 23S rRNA Gene Sequencing as a Supplementary Diagnostic Method"],"prefix":"10.2174","volume":"2","author":[{"given":"Claus","family":"Moser","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keld","family":"Andresen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Kjerulf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suheil","family":"Salamon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kemp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens J\u00f8rgen","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"refR1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1128\/JCM.38.1.32-39.2000","volume":"38","author":"Rantakokko-Jalava K","year":"2000","unstructured":"Rantakokko-Jalava K, Nikkari S, Jalava J, et al. \n                    Direct amplification of rRNA genes in diagnosis of bacterial infections \n                    J Clin Microbiol  \n                    2000; \n                    38\n                    : 32-9.","journal-title":"J Clin Microbiol"},{"key":"refR2","first-page":"19","volume":"70","author":"Harmsen D","year":"2004","unstructured":"Harmsen D, Karch H. \n                    16S rDNA diagnosing pathogens a living tree \n                    ASM News  \n                    2004; \n                    70\n                    : 19-24.","journal-title":"ASM News"},{"key":"refR3","unstructured":"Dostal S, Richter E, Harmsen D. \n                    Concise guide to mycobacteria and their molecular differentiation. \n                     Norderstedt, Germany: BoD GmbH 2003."},{"key":"refR4","doi-asserted-by":"crossref","first-page":"2198","DOI":"10.1002\/1529-0131(199910)42:10<2198::AID-ANR23>3.0.CO;2-N","volume":"42","author":"Heijden IM","year":"1999","unstructured":"Heijden IM, van der Wilbrink B, Vije AEM, Schouls LM, Breedveld FC, Tak PP. \n                    Detection of bacterial DNA in serial synovial samples obtained during antibiotic treatment from patients with septic arthritis \n                    Arthritis Rheum  \n                    1999; \n                    42\n                    : 2198-03.","journal-title":"Arthritis Rheum"},{"key":"refR5","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1001\/jama.297.13.1478","volume":"297","author":"Margaretten ME","year":"2007","unstructured":"Margaretten ME, Kohlwes J, Moore D, Bent S, et al. \n                    Does this adult patient have septic arthritis? \n                    JAMA  \n                    2007; \n                    297\n                    : 1478-88.","journal-title":"JAMA"},{"key":"refR6","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.1128\/JCM.40.9.3449-3454.2002","volume":"40","author":"Yang S","year":"2002","unstructured":"Yang S, Lin S, Kelen GD, et al. \n                    Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens \n                    J Clin Microbiol  \n                    2002; \n                    40\n                    : 3449-54.","journal-title":"J Clin Microbiol"},{"key":"refR7","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/j.1469-0691.2006.01366.x","volume":"12","author":"Smith JW","year":"2006","unstructured":"Smith JW, Chalupa P, Shabaz Hasan M. \n                    Infectious arthritis clinical features, laboratory findings and treatment \n                    Clin Microbiol Infect  \n                    2006; \n                    12\n                    : 309-14.","journal-title":"Clin Microbiol Infect"},{"key":"refR8","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.berh.2006.08.001","volume":"20","author":"Tarkowski A","year":"2006","unstructured":"Tarkowski A. \n                    Infectious arthritis \n                    Best Pract Res Clin Rheumatol  \n                    2006; \n                    20\n                    : 1029-44.","journal-title":"Best Pract Res Clin Rheumatol"},{"key":"refR9","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"Altschul SF","year":"1997","unstructured":"Altschul SF, Madden TL, Sc\u00e4ffer AA. \n                    Gapped BLAST and PSI-BLAST: A new generation of protein database search programs \n                    Nucleic Acid Res  \n                    1997; \n                    25\n                    : 3389-402.","journal-title":"Nucleic Acid Res"},{"key":"refR10","doi-asserted-by":"crossref","first-page":"S91","DOI":"10.1016\/S0929-693X(07)80041-8","volume":"14","author":"Ferroni A","year":"2007","unstructured":"Ferroni A. \n                    Epidemiology and bacteriological diagnosis of paediatric acute osteoarticular infections \n                    Arch Pediatr  \n                    2007; 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\n                    26\n                    : 377-81.","journal-title":"Ped Infect Dis J"},{"key":"refR13","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1136\/ard.60.3.287","volume":"60","author":"Jalava J","year":"2001","unstructured":"Jalava J, Skurnik M, Toivanen A, Toivanen P, Eerola E. \n                    Bacterial PCR in the diagnosis of joint infection \n                    Ann Rheum Dis  \n                    2001; \n                    60\n                    : 287-89.","journal-title":"Ann Rheum Dis"},{"key":"refR14","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1128\/JCM.37.10.3281-3290.1999","volume":"37","author":"Tunney MM","year":"1999","unstructured":"Tunney MM, Patrick S, Curran MD. \n                    Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene \n                    J Clin Microbiol  \n                    1999; \n                    37\n                    : 3281-90.","journal-title":"J Clin Microbiol"},{"key":"refR15","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1128\/JCM.42.2.497-504.2004","volume":"42","author":"Drancourt M","year":"2004","unstructured":"Drancourt M, Roux V, Fournier P-E, Raoult D. \n                    rpoB gene sequence-based identification of aerobic Gram-positive cocci of the genera Streptococci, Enterococcus, Gemmela, Abiotrophia, and Granulicatella \n                    J Clin Microbiol  \n                    2004; \n                    42\n                    : 497-504.","journal-title":"J Clin Microbiol"},{"key":"refR16","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1128\/JCM.41.2.763-766.2003","volume":"41","author":"Gauduchon V","year":"2003","unstructured":"Gauduchon V, Chalabreysse L, Etienne J, et al. \n                    Molecular diagnosis of infective endocarditis by PCR amplification and direct sequencing of DNA from valve tissue \n                    J Clin Microbiol  \n                    2003; \n                    41\n                    : 763-6.","journal-title":"J Clin Microbiol"},{"key":"refR17","unstructured":"Lepp PW, Relman DA. \n                    \n                    Molecular phylogenetic analysis  In: Persing DH, Tenover FC, Versalovic J, Eds. \n                    (Chapter 13) Molecular Microbiology Diagnostic Principles and Practice. \n                    Washington DC: ASM Press 2004."}],"container-title":["The Open Microbiology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/openmicrobiologyjournal.com\/contents\/volumes\/V2\/TOMICROJ-2-85\/TOMICROJ-2-85.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openmicrobiologyjournal.com\/contents\/volumes\/V2\/TOMICROJ-2-85\/TOMICROJ-2-85.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openmicrobiologyjournal.com\/contents\/volumes\/V2\/TOMICROJ-2-85\/TOMICROJ-2-85.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T06:40:09Z","timestamp":1761547209000},"score":1,"resource":{"primary":{"URL":"https:\/\/openmicrobiologyjournal.com\/VOLUME\/2\/PAGE\/85\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,13]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,6,13]]}},"alternative-id":["TOMICROJ-2-85"],"URL":"https:\/\/doi.org\/10.2174\/1874285800802010085","relation":{},"ISSN":["1874-2858"],"issn-type":[{"type":"print","value":"1874-2858"}],"subject":[],"published":{"date-parts":[[2008,6,13]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Double blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2008-5-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-5-23","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-5-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-06-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}