{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T03:00:35Z","timestamp":1771470035215,"version":"3.50.1"},"reference-count":40,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2014,10,31]],"date-time":"2014-10-31T00:00:00Z","timestamp":1414713600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["openmicrobiologyjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TOMICROJ"],"published-print":{"date-parts":[[2014,10,31]]},"abstract":"\n Background: <\/jats:title>\n Caveolin-1 (Cav-1) is the major protein of the caveolae and plays a role in multiple cellular functions and implicated to have anti-HIV activity. Regulated expression of Cav-1 is important for safe and effective use in order to exploit Cav-1 for HIV therapeutic applications.<\/jats:p>\n <\/jats:sec>\n \n Methods: <\/jats:title>\n A series of Cav-1 and GFP expression vectors were constructed under the control of the HIV LTR for conditional expression or CMV promoter and the expression of Cav-1 was monitored in the presence or absence of Tat or HIV infection in order to establish the restricted expression of Cav-1 to HIV infected cells. <\/jats:p>\n <\/jats:sec>\n \n Results: <\/jats:title>\n Cav-1 expression was evident under the control of the HIV LTR in the absence of Tat or HIV infection as demonstrated by immunoblot. Placing two internal ribosomal entry sequences (IRES) and a Rev response element, RRE (5\u2019~ LTR-IRES-GFP-RRE-IRES-Cav-1~3\u2019) resulted in no expression of Cav-1 in the absence of Tat with effective expression in the presence of Tat. Transduction of HIV permissive cells with this construct using a foamy virus vector show that Cav-1 was able to inhibit HIV replication by 82%. Cells that received LTR-IRES-GFP-RRE-IRES-Cav-1 remain healthy in the absence of Tat or HIV infection. <\/jats:p>\n <\/jats:sec>\n \n Conclusion: <\/jats:title>\n These results taken together reveal the inclusion of two IRES establishes a significant reduction of leak through expression of Cav-1 in the absence of Tat or HIV infection. Such regulated expression will have therapeutic application of Cav-1 for HIV infection as well as broad applications which can be beneficial for other host-targeted interventions as therapeutics.<\/jats:p>\n <\/jats:sec>","DOI":"10.2174\/1874285801408010114","type":"journal-article","created":{"date-parts":[[2014,11,14]],"date-time":"2014-11-14T09:17:02Z","timestamp":1415956622000},"page":"114-121","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishing Restricted Expression of Caveolin-1 in HIV Infected Cells and Inhibition of Virus Replication"],"prefix":"10.2174","volume":"8","author":[{"given":"Yung-Tsun","family":"Lo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter E","family":"Nadeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayalew","family":"Mergia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"refR1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1083\/jcb.118.5.1003","volume":"118","author":"Kurzchalia TV","year":"1992","unstructured":"Kurzchalia TV, Dupree P, Parton RG , et al. \n VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles. \n J Cell Biol \n 1992; \n 118\n : 1003-14.","journal-title":"J Cell Biol."},{"key":"refR2","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1002\/j.1460-2075.1993.tb05804.x","volume":"2","author":"Dupree P","year":"1993","unstructured":"Dupree P, Parton RG, Raposo G, Kurzchalia TV, Simons K. \n Caveolae and sorting in the trans-Golgi network of epithelial cells. \n EMBO J \n 1993; \n 2\n : 1597-605.","journal-title":"EMBO J."},{"key":"refR3","first-page":"1424","volume":"24","author":"Palade GEFsobc","year":"1953","unstructured":"Palade GEFsobc. \n Fine structure of blood capillaries. \n J Appl Phys \n 1953; \n 24\n : 1424.","journal-title":"J Appl Phys."},{"key":"refR4","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1083\/jcb.1.5.445","volume":"1","author":"Yamada E","year":"1955","unstructured":"Yamada E. \n The fine structures of the gall bladder epithelium of the mouse. \n J Biophys Biochem Cytol \n 1955; \n 1\n : 445-58.","journal-title":"J Biophys Biochem Cytol."},{"key":"refR5","first-page":"334","volume":"1746","author":"Stan RV","year":"2005","unstructured":"Stan RV. \n Structure of caveolae. \n Biochem Biophys \n 2005; \n 1746\n : 334-48.","journal-title":"Biochem Biophys."},{"key":"refR6","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.1091\/mbc.e03-08-0593","volume":"15","author":"Stan RV","year":"2004","unstructured":"Stan RV, Tkachenko E, Niesman IR. \n PV1 is a key structural component for the formation of the stomatal and fenestral diaphragms. \n Mol Biol Cell \n 2004; \n 15\n : 3615-30.","journal-title":"Mol Biol Cell."},{"key":"refR7","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/nrm2122","volume":"8","author":"Parton RG","year":"2007","unstructured":"Parton RG, Simons K. \n The multiple faces of caveolae. \n Nat Rev Mol Cell Biol \n 2007; \n 8\n : 185-94.","journal-title":"Nat Rev Mol Cell Biol."},{"key":"refR8","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3109\/09687689509038506","volume":"12","author":"Lisanti MP","year":"1995","unstructured":"Lisanti MP, Tang Z, Scherer PE, K\u00fcbler E, Koleske AJ, Sargiacomo M. \n Caveolae, transmembrane signalling and cellular transformation. \n Mol Membr Biol \n 1995; \n 12\n : 121-4.","journal-title":"Mol Membr Biol."},{"key":"refR9","doi-asserted-by":"crossref","first-page":"5419","DOI":"10.1074\/jbc.273.10.5419","volume":"273","author":"Okamoto T","year":"1998","unstructured":"Okamoto T, Schlegel A, Scherer PE, Lisanti MP. \n Caveolins, a family of scaffolding proteins for organizing \u201cpreassembled signaling complexes\u201d at the plasma membrane. \n J Biol Chem \n 1998; \n 273\n : 5419-22.","journal-title":"J Biol Chem."},{"key":"refR10","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/S0955-0674(99)80061-1","volume":"11","author":"Kurzchalia TV","year":"1999","unstructured":"Kurzchalia TV, Parton RG. \n Membrane microdomains and caveolae. \n Curr Opin Cell Biol \n 1999; \n 11\n : 424-31.","journal-title":"Curr Opin Cell Biol."},{"key":"refR11","doi-asserted-by":"crossref","first-page":"41295","DOI":"10.1074\/jbc.R200020200","volume":"277","author":"Liu P","year":"2002","unstructured":"Liu P, Rudick M, Anderson RG. \n Multiple functions of caveolin-1. \n J Biol Chem \n 2002; \n 277\n : 41295-8.","journal-title":"J Biol Chem."},{"key":"refR12","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1126\/science.289.5480.785","volume":"289","author":"Shin JS","year":"2000","unstructured":"Shin JS, Z\n Gao. \n , Abraham, SN. Involvement of cellular caveolae in bacterial entry into mast cells. \n Science \n 2000; \n 289\n : 785-88.","journal-title":"Science."},{"key":"refR13","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/35074539","volume":"3","author":"Pelkmans L","year":"2001","unstructured":"Pelkmans L, Kartenbeck J, Helenius A. \n Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER. \n Nature Cell Biol \n 2001; \n 3\n : 473-83.","journal-title":"Nature Cell Biol"},{"key":"refR14","doi-asserted-by":"crossref","first-page":"C494","DOI":"10.1152\/ajpcell.00458.2004","volume":"288","author":"Williams TM","year":"2005","unstructured":"Williams TM, Lisanti MP. \n Caveolin-1 in oncogenic transformation, cancer, and metastasis. \n Am J Physiol Cell Physiol \n 2005; \n 288\n : C494-506.","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"refR15","doi-asserted-by":"crossref","first-page":"C242","DOI":"10.1152\/ajpcell.00185.2008","volume":"295","author":"Frank PG","year":"2008","unstructured":"Frank PG, Pavlides S, Cheung MW, Daumer K, Lisanti MP. \n Role of caveolin-1 in the regulation of lipoprotein metabolism. \n Am J Physiol Cell Physiol \n 2008; \n 295\n : C242-8.","journal-title":"Am J Physiol Cell Physiol."},{"key":"refR16","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s00441-008-0659-8","volume":"335","author":"Frank PG","year":"2009","unstructured":"Frank PG, Pavlides S, Lisanti MP. \n Caveolae and transcytosis in endothelial cells role in atherosclerosis. \n Cell Tissue Res \n 2009; \n 335\n : 41-7.","journal-title":"Cell Tissue Res."},{"key":"refR17","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1194\/jlr.R200005-JLR200","volume":"44","author":"Gargalovic P","year":"2002","unstructured":"Gargalovic P, Dory L. \n Caveolins and macrophage lipid metabolism. \n J Lipid Res \n 2002; \n 44\n : 11-21.","journal-title":"J Lipid Res."},{"key":"refR18","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/S1471-4906(01)02161-5","volume":"23","author":"Harris J","year":"2002","unstructured":"Harris J, Werling D, Hope JC, Taylor G, Howard CJ. \n Caveolae and caveolin in immune cells distribution and functions. \n Trends Immunol \n 2002; \n 23\n : 158-64.","journal-title":"Trends Immunol."},{"key":"refR19","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1046\/j.1365-2567.2002.01362.x","volume":"105","author":"Harris J","year":"2002","unstructured":"Harris J, Werling D, Koss M, Monaghan P, Taylor G, Howard CJ. \n Expression of caveolin by bovine lymphocytes and antigen-presenting cells. \n Immunology \n 2002; \n 105\n : 190-5.","journal-title":"Immunology."},{"key":"refR20","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1139\/o03-071","volume":"82","author":"Quest AFG","year":"2004","unstructured":"Quest AFG, Leyton L, P\u00e1rraga M. \n Caveolins, caveolae, and lipid rafts in cellular transport, signaling, and disease. \n Biochem Cell Biol \n 2004; \n 82\n : 129-44.","journal-title":"Biochem. Cell Biol."},{"key":"refR21","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/labinvest.2009.23","volume":"89","author":"Mercier I","year":"2009","unstructured":"Mercier I, Jasmin J-F, Pavlides S , et al. \n Clinical and translational implications of the caveolin gene family lessons from mouse models and human genetic disorders. \n Lab Investig \n 2009; \n 89\n : 614-23.","journal-title":"Lab Investig."},{"key":"refR22","doi-asserted-by":"crossref","first-page":"6525","DOI":"10.1074\/jbc.272.10.6525","volume":"272","author":"Couet J","year":"1997","unstructured":"Couet J, Li S, Okamoto T, Ikezu T, Lisanti MP. \n Identification of peptide and protein ligands for the caveolin-scaffolding domain.Implications for the interaction of caveolin with caveolae-associated proteins. \n J Biol Chem \n 1997; \n 272\n : 6525-33.","journal-title":"J Biol Chem."},{"key":"refR23","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.immuni.2004.08.015","volume":"21","author":"Hovanessian AG","year":"2004","unstructured":"Hovanessian AG, Briand JP, Said AS , et al. \n The caveolin-1 binding domain of HIV-1 glycoprotein gp41 is an efficient B-cell epitope vaccine candidate against virus infection. \n Immunity \n 2004; 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\n 85\n : 5483-93.","journal-title":"J Virol."},{"key":"refR30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/1742-4690-9-85","volume":"9","author":"Lin S","year":"2012","unstructured":"Lin S, Nadeau PE, Wang X, Mergia A. \n Caveolin-1 reduces HIV-1 infectivity by restoration of HIV Nef mediated impairment of cholesterol efflux by apoA-I. \n Retrovirology \n 2012; \n 9\n : 85.","journal-title":"Retrovirology."},{"key":"refR31","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.virol.2012.05.016","volume":"432","author":"Simmons GE","year":"2012","unstructured":"Simmons GE Jr, Taylor HE, Hildreth JE. \n Caveolin-1 suppresses human immunodeficiency virus-1 replication by inhibiting acetylation of NF-kappaB. \n Virology \n 2012; \n 432\n : 110-9.","journal-title":"Virology."},{"key":"refR32","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.virusres.2008.11.016","volume":"140","author":"Park J","year":"2009","unstructured":"Park J, Nadeau PE, Mergia A. \n Activity of TAR in inducible inhibition of HIV replication by foamy virus vector expressing siRNAs under the control of HIV LTR. \n Virus Res \n 2009; \n 140\n : 112-20.","journal-title":"Virus Res."},{"key":"refR33","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.virol.2005.08.038","volume":"343","author":"Park J","year":"2005","unstructured":"Park J, Nadeau P, Zucali Jr, Johnson CM, Mergia A. \n Inhibition of simian immunodeficiency virus by foamy virus vectors expressing siRNAs. \n Virology \n 2005; \n 343\n : 275-82.","journal-title":"Virology."},{"key":"refR34","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1006\/viro.2000.0773","volume":"280","author":"Mergia A","year":"2001","unstructured":"Mergia A, Soumya C, Kolson DL, Goodenow MM, Ciccarone T. \n The efficiency of simian foamy virus vector type-1 (SFV-1):in non-dividing cells and in human PBLs. \n Virology \n 2001; \n 280\n : 243-52.","journal-title":"Virology."},{"key":"refR35","doi-asserted-by":"crossref","first-page":"9487","DOI":"10.1128\/JVI.00763-10","volume":"84","author":"Lin S X-MW","year":"2010","unstructured":"Lin S X-MW, Nadeau PE, Mergia A. \n HIV infection upregulates Caveolin 1 (Cav-1):expression to restrict virus production. \n J Virol \n 2010; \n 84\n : 9487-96.","journal-title":"J Virol."},{"key":"refR36","doi-asserted-by":"crossref","first-page":"11103","DOI":"10.1073\/pnas.93.20.11103","volume":"93","author":"Dropulic B","year":"1996","unstructured":"Dropulic B, Hermankova M, Pitha P. \n A conditionally replicating HIV-1 vector interferes with wild-type HIV-1 replication and spread. \n Proc Natl Acad Sci USA \n 1996; \n 93\n : 11103-8.","journal-title":"Proc Natl Acad Sci USA."},{"key":"refR37","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1089\/hum.1997.8.9-1115","volume":"8","author":"Paik SY","year":"1997","unstructured":"Paik SY, Banerjea A, Chen CJ, Ye Z, Harmison GG, Schubert M. \n Defective HIV-1 provirus encoding a multi-target ribozyme inhibits accumulation of spliced and unspliced HIV-1 mRNAs, reduces infectivity of viral progeny, and protects the cells from pathogenesis. \n Hum Gene Ther \n 1997; \n 8\n : 1115-23.","journal-title":"Hum Gene Ther."},{"key":"refR38","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1128\/JVI.80.4.1863-1873.2006","volume":"80","author":"Unwalla HJ","year":"2006","unstructured":"Unwalla HJ, Li HT, Bahner I, Li MJ, Kohn D, Rossi JJ. \n Novel pol ii fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin rna and protein. \n J Virol \n 2006; \n 80\n : 1863-73.","journal-title":"J Virol."},{"key":"refR39","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1038\/nbt1040","volume":"22","author":"Unwalla HJ","year":"2004","unstructured":"Unwalla HJ, Li MJ, Kim JD , et al. \n Negative feedback inhibition of HIV-1 by TAT-inducible expression of siRNA. \n Nat Biotechnol \n 2004; \n 22\n : 1573-8.","journal-title":"Nat Biotechnol."},{"key":"refR40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1586\/eri.12.146","volume":"11","author":"Sloan RD","year":"2013","unstructured":"Sloan RD, Wainberg MA. \n Harnessing the therapeutic potential of host antiviral restriction factors that target HIV. \n Expert Rev Anti Infect Ther \n 2013; \n 11\n : 1-4.","journal-title":"Expert Rev Anti Infect Ther."}],"container-title":["The Open Microbiology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/openmicrobiologyjournal.com\/contents\/volumes\/V8\/TOMICROJ-8-114\/TOMICROJ-8-114.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openmicrobiologyjournal.com\/contents\/volumes\/V8\/TOMICROJ-8-114\/TOMICROJ-8-114.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openmicrobiologyjournal.com\/contents\/volumes\/V8\/TOMICROJ-8-114\/TOMICROJ-8-114.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T07:53:58Z","timestamp":1556092438000},"score":1,"resource":{"primary":{"URL":"https:\/\/openmicrobiologyjournal.com\/VOLUME\/8\/PAGE\/114\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,31]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,10,31]]}},"alternative-id":["TOMICROJ-8-114"],"URL":"https:\/\/doi.org\/10.2174\/1874285801408010114","relation":{},"ISSN":["1874-2858"],"issn-type":[{"value":"1874-2858","type":"print"}],"subject":[],"published":{"date-parts":[[2014,10,31]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2014-6-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-9-22","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-2","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}