{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T05:33:29Z","timestamp":1783056809269,"version":"3.54.6"},"reference-count":39,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2015,8,31]],"date-time":"2015-08-31T00:00:00Z","timestamp":1440979200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["openmicrobiologyjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TOMICROJ"],"published-print":{"date-parts":[[2015,8,31]]},"abstract":"<jats:p><jats:underline>Background<\/jats:underline>:<jats:italic>Chlamydia trachomatis<\/jats:italic>is recognized as one of the most common sexually transmitted pathogen in the world. 50-80% of infected females are asymptomatic. These untreated women are at risk of developing chronic sequelae leading to tubal pathology causing infertility. Infertility is defined as 1 year of unprotected intercourse without pregnancy. It may be primary or secondary.<jats:italic><jats:underline>Aim<\/jats:underline>:<\/jats:italic>To find out the association of genital<jats:italic>Chlamydia trachomatis<\/jats:italic>infection with female infertility.<jats:italic><jats:underline>Materials and Methodology<\/jats:underline>:<\/jats:italic>This case control study has been carried out in collaboration with R. G. Kar Medical College and Institute of Post Graduate Medical Education &amp;amp; Research, India, between July 2012 and June 2013. 40 infertile and 40 pregnant women were enrolled by purposive sampling as per inclusion and exclusion criteria. ELISA test was performed to detect serum IgG and IgA antibody against recombinant analogs of MOMP and 3 different PCR assays were done targeting MOMP and rRNA DNA from DNA extracted from first void urine.<jats:italic><jats:underline>Results<\/jats:underline>:<\/jats:italic>IgG seropositivity was significantly higher (15%<jats:italic>vs<\/jats:italic>0%,<jats:italic>P<\/jats:italic>=.0255) in cases than controls, though there was no significant difference in the proportion of IgA seropositivity among 2 groups (12.5%<jats:italic>vs<\/jats:italic>2.5%,<jats:italic>P<\/jats:italic>=0.2007). Out of 80 samples 2 samples showed the production of amplicons with R1 \u2013 R2 primers. Only 1 sample gave positive result with production of amplicons with all the 3 primers used (R1 \u2013 R2, CT0005 \u2013 CT06 and JM15 \u2013 JM16).<jats:italic><jats:underline>Conclusion<\/jats:underline>:<\/jats:italic>Persistent<jats:italic>C. trachomatis<\/jats:italic>infection must be recognized as a risk factor of infertility in this region of India. The low PCR positivity in FVU sample helps to conclude the diagnostic utility of serological tests in screening of infertile women.<\/jats:p>","DOI":"10.2174\/1874285801509010110","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T10:45:58Z","timestamp":1442400358000},"page":"110-116","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Association of Genital Chlamydia trachomatis Infection with Female Infer-tility, Study in a Tertiary Care Hospital in Eastern India"],"prefix":"10.2174","volume":"9","author":[{"given":"Mallika","family":"Ghosh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhadip","family":"Choudhuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reena Ghosh","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basudev","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujata","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"refR1","unstructured":"Prevalence and incidence of selected sexually transmitted infections, methods and results used by WHO to generate 2005 estimates 2011."},{"key":"refR2","unstructured":"Summary of a review of literature: programs to promote Chlamydia screening 2007."},{"key":"refR3","unstructured":"Charlotte GA, Thomas QC. Chlamydial Infections In: Dan LL, Anthony FS, Dennis KL, Stephen HL, Jameson JL, Joseph L, Eds. Harrison\u2019s Principles of Internal Medicine. USA: McGraw Hill 2012; pp. 1421-31."},{"key":"refR4","doi-asserted-by":"crossref","unstructured":"Walter SE, Byron BE. Chlamydia trachomatis (Trachoma, Perinatal Infections, LymphogranulomaVenereum, and other genital infections) In: Gerald ML, John BE, Rephael D, Eds. Mandell, Douglas & Bennett\u2019s Principles & Practice of Infectious Diseases. UK: Churchill Livingstone Elsevier 2010; pp. 2443-57.","DOI":"10.1016\/B978-0-443-06839-3.00180-6"},{"key":"refR5","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1093\/humrep\/15.5.1079","volume":"15","author":"Thomas K.","year":"2000","unstructured":"Thomas K, Coughlin L, Mannion PT, Haddad NG. The value of Chlamydia trachomatis antibody testing as part of routine infertility investigations Hum Reprod 2000; 15 (5) : 1079-82.","journal-title":"Hum. Reprod"},{"key":"refR6","unstructured":"Mira A, Richard BO, Danny SJ, et al. Infertility and assisted reproductive technology In: Jonathen BS, Ed. Berek & Novak\u2019s Gynaecology. USA: Lippincott William Wilkins 2012; pp. 1133-89."},{"key":"refR7","unstructured":"World health Organization Infecundity, Infertility and Childlessness in developing countries DHS Comparative Reports no 9 Calverton, Maryland, USA: ORC Macro and the World Health Organization 2004 [Cited 2011 November 25] Available from: http:\/\/wwwwhoint\/reproductivehealth\/topics\/infertility\/DHSCR9 pdf 2004."},{"key":"refR8","first-page":"440","volume":"134","author":"Adamson P.C.","year":"2011","unstructured":"Adamson PC, Krupp K, Freeman AH, Klausner JD, Reingold AL, Madhivanan P. Prevalence & correlates of primary infertility among young women in Mysore, India Indian J Med Res 2011; 134 : 440-6.","journal-title":"Indian J. Med. Res"},{"key":"refR9","doi-asserted-by":"crossref","unstructured":"Srivastava P, Jha R, Bas S, et al. In Infertile women, cells from Chlamydia trachomatis infected site release higher levels of interferon gamma, interleukin 10, and tumor necrosis factor alpha, upon heat shock protein stimulation than fertile women Reprod Biol Endocrinol 2008 [Cited 2011 January 5] Available from: http:\/\/wwwrbejcom\/content\/6\/1\/20 2008.","DOI":"10.1186\/1477-7827-6-20"},{"key":"refR10","doi-asserted-by":"publisher","first-page":"S104","DOI":"10.1086\/652402","volume":"201","author":"Geisler W.M.","year":"2010","unstructured":"Geisler WM. Duration of untreated, uncomplicated Chlamydia trachomatis genital infection and factors associated with chlamydia resolution: a review of human studies J Infect Dis 2010; 201 (S2) (Suppl. 2) : S104-13.","journal-title":"J. Infect. Dis"},{"key":"refR11","doi-asserted-by":"publisher","first-page":"S178","DOI":"10.1086\/652400","volume":"201","author":"Batteiger B.E.","year":"2010","unstructured":"Batteiger BE, Xu F, Johnson RE, Rekart ML. Protective immunity to Chlamydia trachomatis genital infection: evidence from human studies J Infect Dis 2010; 201 (S2) (Suppl. 2) : S178-89.","journal-title":"J. Infect. Dis"},{"key":"refR12","doi-asserted-by":"publisher","first-page":"S114","DOI":"10.1086\/652397","volume":"201","author":"Darville T.","year":"2010","unstructured":"Darville T, Hiltke TJ. Pathogenesis of genital tract disease due to Chlamydia trachomatis. J Infect Dis 2010; 201 (S2) (Suppl. 2) : S114-25.","journal-title":"J. Infect. Dis"},{"key":"refR13","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1155\/S1064744996000336","volume":"4","author":"Witkin S.S.","year":"1996","unstructured":"Witkin SS, Jeremias J, Neuer A, et al. Immune recognition of the 60kD heat shock protein: implications for subsequent fertility Infect Dis Obstet Gynecol 1996; 4 (3) : 152-8.","journal-title":"Infect. Dis. Obstet. Gynecol"},{"key":"refR14","unstructured":"Mardh PA. Chlamydia In: Peter BS, Patrick MR, Guido F, Eds. Topley & Wilson\u2019s Microbiology & Microbial Infections. UK: Hodder Arnold 2005; pp. 2006-25."},{"key":"refR15","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1093\/humupd\/5.5.433","volume":"5","author":"Paavonen J.","year":"1999","unstructured":"Paavonen J, Eggert-Kruse W. Chlamydia trachomatis: impact on human reproduction Hum Reprod Update 1999; 5 (5) : 433-47.","journal-title":"Hum. Reprod. Update"},{"key":"refR16","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1093\/humrep\/deh710","volume":"20","author":"den Hartog J.E.","year":"2005","unstructured":"den Hartog JE, Land JA, Stassen FR, Kessels AG, Bruggeman CA. Serological markers of persistent C. trachomatis infections in women with tubal factor subfertility Hum Reprod 2005; 20 (4) : 986-90.","journal-title":"Hum. Reprod"},{"key":"refR17","doi-asserted-by":"publisher","first-page":"S85","DOI":"10.1086\/652392","volume":"201","author":"Gottlieb S.L.","year":"2010","unstructured":"Gottlieb SL, Brunham RC, Byrne GI, Martin DH, Xu F, Berman SM. Introduction: The natural history and immunobiology of Chlamydia trachomatis genital infection and implications for chlamydia control J Infect Dis 2010; 201 (S2) (Suppl. 2) : S85-7.","journal-title":"J. Infect. Dis"},{"key":"refR18","doi-asserted-by":"publisher","unstructured":"Fleiss JL, Levin B, Paik MC. Statistical Methods for Rates and Proportions. 3rd ed. New York: John Wiley & Sons 2003.","DOI":"10.1002\/0471445428"},{"key":"refR19","first-page":"770","volume":"123","author":"Malik A.","year":"2006","unstructured":"Malik A, Jain S, Hakim S, Shukla I, Rizvi M. Chlamydia trachomatis infection & female infertility Indian J Med Res 2006; 123 (6) : 770-5.","journal-title":"Indian J. Med. Res"},{"key":"refR20","first-page":"26","volume":"1","author":"Dudala S.R.","year":"2013","unstructured":"Dudala SR, Arlappa N. An Updated Prasad\u2019s Socio Economic Status Classification for 2013 Int J Res Dev Health 2013; 1 (2) : 26-7.","journal-title":"Int J Res Dev Health"},{"key":"refR21","first-page":"20","volume":"34","author":"Fallah F.","year":"2005","unstructured":"Fallah F, Kazemi B, Goudarzi H, et al. Detection of Chlamydia trachomatis from urine specimens by PCR in women with cervicitis Iran J Public Health 2005; 34 (2) : 20-6.","journal-title":"Iran. J. Public Health"},{"key":"refR22","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1128\/JCM.28.9.1968-1973.1990","volume":"28","author":"Bobo L.","year":"1990","unstructured":"Bobo L, Coutlee F, Yolken RH, Quinn T, Viscidi RP. Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay J Clin Microbiol 1990; 28 (9) : 1968-73.","journal-title":"J. Clin. Microbiol"},{"key":"refR23","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1128\/JCM.31.7.1753-1758.1993","volume":"31","author":"Mahony J.B.","year":"1993","unstructured":"Mahony JB, Luinstra KE, Sellors JW, Chernesky MA. Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids J Clin Microbiol 1993; 31 (7) : 1753-8.","journal-title":"J. Clin. Microbiol"},{"key":"refR24","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1007\/BF01967500","volume":"9","author":"Claas H.C.","year":"1990","unstructured":"Claas HC, Melchers WJ, de Bruijn IH, et al. Detection of Chlamydia trachomatis in clinical specimens by the polymerase chain reaction Eur J Clin Microbiol Infect Dis 1990; 9 (12) : 864-8.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis"},{"key":"refR25","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1128\/JCM.29.1.42-45.1991","volume":"29","author":"Claas H.C.","year":"1991","unstructured":"Claas HC, Wagenvoort JH, Niesters HG, Tio TT, Van Rijsoort-Vos JH, Quint WG. Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study J Clin Microbiol 1991; 29 (1) : 42-5.","journal-title":"J. Clin. Microbiol"},{"key":"refR26","doi-asserted-by":"crossref","first-page":"323","DOI":"10.4269\/ajtmh.2008.78.323","volume":"78","author":"Siemer J.","year":"2008","unstructured":"Siemer J, Theile O, Larbi Y, et al. Chlamydia trachomatis infection as a risk factor for infertility among women in Ghana, West Africa Am J Trop Med Hyg 2008; 78 (2) : 323-7.","journal-title":"Am. J. Trop. Med. Hyg"},{"key":"refR27","doi-asserted-by":"crossref","first-page":"120","DOI":"10.59566\/IJBS.2011.7120","volume":"7","author":"Jeremiah I.","year":"2011","unstructured":"Jeremiah I, Okike O, Akani C. The prevalence of serum immunoglobulin g antibody to Chlamydia trachomatis in subfertile women presenting at the university of port harcourt teaching hospital, Nigeria Int J Biomed Sci 2011; 7 (2) : 120-4.","journal-title":"Int. J. Biomed. Sci"},{"key":"refR28","first-page":"795","volume":"5","author":"Fadwa S.M.","year":"2011","unstructured":"Fadwa SM. Detection of Chlamydia trachomatis among infected women J Basic Appl Sci 2011; 5 (8) : 795-802.","journal-title":"J. Basic Appl. Sci"},{"key":"refR29","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1086\/496979","volume":"41","author":"Paz-Bailey G.","year":"2005","unstructured":"Paz-Bailey G, Rahman M, Chen C, et al. Changes in the etiology of sexually transmitted diseases in Botswana between 1993 and 2002: implications for the clinical management of genital ulcer disease Clin Infect Dis 2005; 41 (9) : 1304-12.","journal-title":"Clin. Infect. Dis"},{"key":"refR30","first-page":"1148","volume":"14","author":"Al-Ramahi M.","year":"2008","unstructured":"Al-Ramahi M, Mahafzah A, Saleh S, Fram K. Prevalence of Chlamydia trachomatis infection in infertile women at a university hospital in Jordan East Mediterr Health J 2008; 14 (5) : 1148-54.","journal-title":"East. Mediterr. Health J"},{"key":"refR31","doi-asserted-by":"publisher","first-page":"3319","DOI":"10.1093\/humrep\/der350","volume":"26","author":"Muvunyi C.M.","year":"2011","unstructured":"Muvunyi CM, Dhont N, Verhelst R, Temmerman M, Claeys G, Padalko E. Chlamydia trachomatis infection in fertile and subfertile women in Rwanda: prevalence and diagnostic significance of IgG and IgA antibodies testing Hum Reprod 2011; 26 (12) : 3319-26.","journal-title":"Hum. Reprod"},{"key":"refR32","first-page":"204","volume":"13","author":"Mania-Pramanik J.","year":"2012","unstructured":"Mania-Pramanik J, Kerkar S, Sonawane S, Mehta P, Salvi V. Current Chlamydia trachomatis infection, a major cause of infertility J Reprod Infertil 2012; 13 (4) : 204-10.","journal-title":"J. Reprod. Infertil"},{"key":"refR33","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1099\/jmm.0.45641-0","volume":"53","author":"Hamdad-Daoudi F.","year":"2004","unstructured":"Hamdad-Daoudi F, Petit J, Eb F. Assessment of Chlamydia trachomatis infection in asymptomatic male partners of infertile couples J Med Microbiol 2004; 53 (Pt 10) : 985-90.","journal-title":"J. Med. Microbiol"},{"key":"refR34","first-page":"777","volume":"55","author":"Joyee A.G.","year":"2007","unstructured":"Joyee AG, Thyagarajan SP, Vikram Reddy E, Rajendran P, Venkatesan C, Ganapathy M. Diagnostic utility of serologic markers for genital chlamydial infection in STD patients in Chennai, India J Assoc Physicians India 2007; 55 : 777-80.","journal-title":"J. Assoc. Physicians India"},{"key":"refR35","first-page":"7","volume":"2","author":"Pratibha G.","year":"2010","unstructured":"Pratibha G, Joseph PI. Incidence of Chlamydia trachomatis infection in infertile urban population J Ecobiotechnol 2010; 2 (3) : 7-11.","journal-title":"J Ecobiotechnol"},{"key":"refR36","first-page":"100","volume":"12","author":"Mawak J.D.","year":"2011","unstructured":"Mawak JD, Dashe N, Agabi YA, et al. Prevalence of genital Chlamydia trachomatis infection among gynecologic clinic attendees in Jos, Nigeria Shiraz E-Med J 2011; 12 (2) : 100-6.","journal-title":"Shiraz E-Med J"},{"key":"refR37","first-page":"15","volume":"44","author":"Shireen J.J.","year":"1998","unstructured":"Shireen JJ. Infertility in India-levels, patterns and consequences: priorities for social science research J Fam Welf 1998; 44 (2) : 15-24.","journal-title":"J. Fam. Welf"},{"key":"refR38","doi-asserted-by":"publisher","first-page":"277","DOI":"10.2147\/IJWH.S46678","volume":"5","author":"Kamel R.M.","year":"2013","unstructured":"Kamel RM. Screening for Chlamydia trachomatis infection among infertile women in Saudi Arabia Int J Womens Health 2013; 5 : 277-84.","journal-title":"Int. J. Womens Health"},{"key":"refR39","first-page":"260","volume":"2","author":"Deshmukh D.G.","year":"2013","unstructured":"Deshmukh DG, Bajaj JK, Damle AS, et al. 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