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The human enzyme has been expressed in<jats:italic>Escherichia coli<\/jats:italic>and<jats:italic>Pichia pastoris<\/jats:italic>in attempt to develop more successful expression systems that allow the production of recombinant IDS for Enzyme Replacement Therapy (ERT). However, the preservation of native signal peptide in the sequence has caused conflicts in processing and recognition in the past, which led to problems in expression and enzyme activity. With the main object being the improvement of the expression system, we eliminate the native signal peptide of human recombinant IDS. The resulting sequence showed two modified codons, thus, our study aimed to analyze computationally the nucleotide sequence of the<jats:italic>IDSnh<\/jats:italic>without signal peptide in order to determine the 3D structure and other biochemical properties to compare them with the native human IDS (<jats:italic>IDSnh<\/jats:italic>). Results showed that there are no significant differences between both molecules in spite of the two-codon modifications detected in the recombinant DNA sequence.<\/jats:p>","DOI":"10.2174\/1874285801610010124","type":"journal-article","created":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T05:14:42Z","timestamp":1464844482000},"page":"124-132","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bioinformatic Analysis of the Human Recombinant Iduronate 2-Sulfate Sulfatase"],"prefix":"10.2174","volume":"10","author":[{"given":"Edwin D.","family":"Morales-\u00c1lvarez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia M.","family":"Rivera-Hoyos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Land\u00e1zuri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal A.","family":"Poutou-Pi\u00f1ales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aura M.","family":"Pedroza-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"965","reference":[{"key":"refR1","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1146\/annurev-med-122313-085916","volume":"66","author":"Parenti G.","year":"2015","unstructured":"Parenti G, Andria G, Ballabio A. 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