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The second strategy is antigen incorporation into the viral capsid proteins. To extend this methodology, we have searched for new sites at the human adenovirus serotype 5 major capsid protein hexon for a vaccine antigen insertion. To this end, we utilized sites in the hexon hypervariable region (HVR) 7, 8 and 9 to display a 15-mer peptide containing the main neutralizing epitope of porcine reproductive and respiratory syndrome virus. However, we could not rescue the viruses with the insertions of the peptide into HVR 8 and 9, consistent with the viruses being unable to tolerate insertions at these sites. In contrast, the virus with the insertion of the peptide in HVR 7 was viable - growing well in cell culture and the inserted peptide was exposed on the virion surface.<\/jats:p>","DOI":"10.2174\/1874357901509010001","type":"journal-article","created":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T07:01:39Z","timestamp":1433401299000},"page":"1-6","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Exploration of New Sites in Adenovirus Hexon for Foreign Peptides Insertion"],"prefix":"10.2174","volume":"9","author":[{"given":"Satyender","family":"Hansra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujit","family":"Pujhari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N","family":"Zakhartchouk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"965","reference":[{"key":"refR1","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1038\/mt.2009.130","volume":"17","author":"Lasaro MO","year":"2009","unstructured":"Lasaro MO, Ertl HC. 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